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Product Info Summary
| SKU: | PA1879 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DDR2 Antibody Picoband®
SKU/Catalog Number
PA1879
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DDR2 Antibody catalog # PA1879. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1879)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human DDR2, different from the related mouse sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1879 is reactive to DDR2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
96736 MW
Background of Ddr2
Discoidin domain-containing receptor 2, also known as CD167b (cluster of differentiation 167b), is a protein that in humans is encoded by the DDR2 gene. This gene encodes a member of the discoidin domain receptor subclass of the receptor tyrosine kinase (RTKs) protein family. RTKs play a key role in the communication of cells with their microenvironment. The encoded protein is a collagen-induced receptor that activates signal transduction pathways involved in cell adhesion, proliferation, and extracellular matrix remodeling. This protein is expressed in numerous cell types and may alos be involved in wound repair and regulate tumor growth and invasiveness. Mutations in this gene are the cause of short limb-hand type spondylometaepiphyseal dysplasia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1879 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human U87 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of DDR2 using anti-DDR2 antibody (PA1879).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR2 antigen affinity purified polyclonal antibody (Catalog # PA1879) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR2 at approximately 120KD. The expected band size for DDR2 is at 97KD.
Protein Target Info & Infographic
Gene/Protein Information For DDR2 (Source: Uniprot.org, NCBI)
Gene Name
DDR2
Full Name
Discoidin domain-containing receptor 2
Weight
96736 MW
Superfamily
protein kinase superfamily
Alternative Names
Discoidin domain-containing receptor 2;Discoidin domain receptor 2;2.7.10.1;CD167 antigen-like family member B;Discoidin domain-containing receptor tyrosine kinase 2;Neurotrophic tyrosine kinase, receptor-related 3;Receptor protein-tyrosine kinase TKT;Tyrosine-protein kinase TYRO10;CD167b;DDR2;NTRKR3, TKT, TYRO10; DDR2 MIG20a, NTRKR3, TKT, TYRO10, WRCN discoidin domain receptor tyrosine kinase 2 discoidin domain-containing receptor 2|CD167 -like family member B|cell migration-inducing protein 20|discoidin domain receptor 2|discoidin domain receptor family, member 2|discoidin domain-containing receptor tyrosine kinase 2|hydroxyaryl-protein kinase|migration-inducing gene 16 protein|neurotrophic tyrosine kinase receptor related 3|receptor protein-tyrosine kinase TKT|tyrosine-protein kinase TYRO10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDR2, check out the DDR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDR2 Antibody Picoband® (PA1879)
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No publications found for PA1879
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Customer Q&As
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16 Customer Q&As for Anti-DDR2 Antibody Picoband®
Question
Do you have a BSA free version of anti-DDR2 antibody PA1879 available?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-DDR2 antibody PA1879 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-10
Question
Will anti-DDR2 antibody PA1879 work on goat WB with adipose tissue?
Z. Mangal
Verified customer
Asked: 2020-04-02
Answer
Our lab technicians have not validated anti-DDR2 antibody PA1879 on goat. You can run a BLAST between goat and the immunogen sequence of anti-DDR2 antibody PA1879 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat adipose tissue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-02
Question
I see that the anti-DDR2 antibody PA1879 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-03
Question
See attached the WB image, lot number and protocol we used for skin using anti-DDR2 antibody PA1879. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-30
Question
Our lab want to know about to test anti-DDR2 antibody PA1879 on human skin for research purposes, then I may be interested in using anti-DDR2 antibody PA1879 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The products we sell, including anti-DDR2 antibody PA1879, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-27
Question
I was wanting to use your anti-DDR2 antibody for WB for human skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skin identification?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
As indicated on the product datasheet, PA1879 anti-DDR2 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-13
Question
Does PA1879 anti-DDR2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
As indicated on the product datasheet, PA1879 anti-DDR2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-DDR2 antibody PA1879. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-19
Question
We have observed staining in human hair follicle dermal papilla uterus. Any tips? Is anti-DDR2 antibody supposed to stain hair follicle dermal papilla uterus positively?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
From literature hair follicle dermal papilla uterus does express DDR2. From Uniprot.org, DDR2 is expressed in adipose tissue, heart thymus, hair follicle dermal papilla uterus, skin, cervix carcinoma, among other tissues. Regarding which tissues have DDR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Hair follicle dermal papilla, and Uterus, Pubmed ID: 14702039
Heart, and Thymus, Pubmed ID: 8247548
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-25
Question
We are currently using anti-DDR2 antibody PA1879 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
T. Carter
Verified customer
Asked: 2019-05-13
Answer
The anti-DDR2 antibody (PA1879) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-13
Question
Will anti-DDR2 antibody PA1879 work for WB with skin?
W. Roberts
Verified customer
Asked: 2019-04-15
Answer
According to the expression profile of skin, DDR2 is highly expressed in skin. So, it is likely that anti-DDR2 antibody PA1879 will work for WB with skin.
Boster Scientific Support
Answered: 2019-04-15
Question
Is a blocking peptide available for product anti-DDR2 antibody (PA1879)?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
We do provide the blocking peptide for product anti-DDR2 antibody (PA1879). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-08-23
Question
Is this PA1879 anti-DDR2 antibody reactive to the isotypes of DDR2?
V. Wu
Verified customer
Asked: 2017-05-31
Answer
The immunogen of PA1879 anti-DDR2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human DDR2(834-855aa RDTKNRPSFQEIHLLLLQQGDE), different from the related mouse sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-31
Question
I am interested in using your anti-DDR2 antibody for positive regulation of osteoblast differentiation studies. Has this antibody been tested with western blotting on u2os whole cell lysates? We would like to see some validation images before ordering.
A. Baker
Verified customer
Asked: 2016-01-12
Answer
Thank you for your inquiry. This PA1879 anti-DDR2 antibody is tested on human placenta tissue, u2os whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-01-12
Question
Our lab were well pleased with the WB result of your anti-DDR2 antibody. However we have observed positive staining in hair follicle dermal papilla uterus cell membrane using this antibody. Is that expected? Could you tell me where is DDR2 supposed to be expressed?
W. Patel
Verified customer
Asked: 2015-08-25
Answer
From what I have seen in literature, hair follicle dermal papilla uterus does express DDR2. Generally DDR2 expresses in cell membrane. Regarding which tissues have DDR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Hair follicle dermal papilla, and Uterus, Pubmed ID: 14702039
Heart, and Thymus, Pubmed ID: 8247548
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-08-25
Question
Can you help my question with product PA1879, anti-DDR2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Li
Verified customer
Asked: 2015-07-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1879 anti-DDR2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-07-30
