Product Info Summary
SKU: | A00311-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-DDIT3 Antibody Picoband™
View all GADD153/CHOP Antibodies
SKU/Catalog Number
A00311-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DDIT3 Antibody Picoband™ catalog # A00311-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDIT3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00311-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DDIT3 recombinant protein (Position: M1-A169).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00311-2 is reactive to DDIT3 in Human
Applications
A00311-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
29 kDa
Calculated molecular weight
74699 MW
Background of GADD153/CHOP
DNA damage-inducible transcript 3, also known as C/EBP homologous protein (CHOP), is a pro-apoptotictranscription factor that is encoded by the DDIT3 gene. It is mapped to 12q13.3. This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
![a00311 2 ddit3 primary antibodies wb testing 1_ a00311 2 ddit3 primary antibodies wb testing 1_](https://www.bosterbio.com/media/catalog/product/a/0/a00311-2-ddit3-primary-antibodies-wb-testing-1_.jpg)
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Figure 1. Western blot analysis of DDIT3 using anti-DDIT3 antibody (A00311-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human THP-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDIT3 antigen affinity purified polyclonal antibody (Catalog # A00311-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDIT3 at approximately 29KD. The expected band size for DDIT3 is at 29KD.
![a00311 2 ddit3 primary antibodies ihc testing 2 a00311 2 ddit3 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a00311-2-ddit3-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of DDIT3 using anti-DDIT3 antibody (A00311-2).
DDIT3 was detected in paraffin-embedded section of human B lymphocytic tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDIT3 Antibody (A00311-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a00311 2 ddit3 primary antibodies ihc testing 3 a00311 2 ddit3 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a00311-2-ddit3-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of DDIT3 using anti-DDIT3 antibody (A00311-2).
DDIT3 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDIT3 Antibody (A00311-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a00311 2 ddit3 primary antibodies ihc testing 4 a00311 2 ddit3 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a00311-2-ddit3-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of DDIT3 using anti-DDIT3 antibody (A00311-2).
DDIT3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDIT3 Antibody (A00311-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a00311 2 ddit3 primary antibodies fc testing 5 a00311 2 ddit3 primary antibodies fc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a00311-2-ddit3-primary-antibodies-fc-testing-5.png)
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Figure 5. Flow Cytometry analysis of K562 cells using anti-DDIT3 antibody (A00311-2).
Overlay histogram showing K562 cells stained with A00311-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDIT3 Antibody (A00311-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
![a00311 2 ddit3 primary antibodies fc testing 6 a00311 2 ddit3 primary antibodies fc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a00311-2-ddit3-primary-antibodies-fc-testing-6.png)
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Figure 6. Flow Cytometry analysis of THP-1 cells using anti-DDIT3 antibody (A00311-2).
Overlay histogram showing THP-1 cells stained with A00311-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDIT3 Antibody (A00311-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For DDIT3 (Source: Uniprot.org, NCBI)
Gene Name
DDIT3
Full Name
DDIT3 upstream open reading frame protein
Weight
74699 MW
Alternative Names
C/EBP-homologous protein 10; C/EBP-homologous protein; CCAAT/enhancer-binding protein homologous protein; CEBP zeta; CHOP; CHOP10 CEBPZ; CHOP10; CHOP-10; CHOPC/EBP zeta; DDIT3; DDIT-3; DNA damage-inducible transcript 3 protein; DNA-damage-inducible transcript 3; GADD153; Growth arrest and DNA damage-inducible protein GADD153 DDIT3 AltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10, CHOP10, GADD153 DNA damage inducible transcript 3 DNA damage-inducible transcript 3 protein|C/EBP zeta|CCAAT/enhancer-binding protein homologous protein|alternative DDIT3 protein|c/EBP-homologous protein 10|growth arrest and DNA damage-inducible protein GADD153
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDIT3, check out the DDIT3 Infographic
![DDIT3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDIT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDIT3 Antibody Picoband™ (A00311-2)
Hello CJ!
A00311-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Tension induces intervertebral disc degeneration via endoplasmic reticulum stress-mediated autophagy
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