Anti-XBP-1 Monoclonal Antibody [3H1G4]

Product Name

Anti-XBP-1 Monoclonal Antibody [3H1G4]

View all XBP1 Antibodies

SKU/Catalog Number

M00234-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-XBP-1 Monoclonal Antibody [3H1G4] (Catalog # M00234-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

XBP-1 monoclonal antibody can be stored at -20°C, stable for one year.

Cite This Product

Anti-XBP-1 Monoclonal Antibody [3H1G4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00234-1)

Host

Mouse

Contents

XBP-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

Clone: 3H1G4

Isotype

IgG1

Immunogen

Mouse monoclonal XBP-1 antibody was raised against recombinant protein corresponding to amino acids 2 to 160 of human XBP-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Biotin-GAPDH antibody is human, mouse, rat, and rabbit reactive.

Reactive Species

M00234-1 is reactive to XBP1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

28695 MW

Background of XBP1

X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00234-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.
Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For XBP1 (Source: Uniprot.org, NCBI)

Alternative Names

XBP2, TREB5, XBP-1 XBP1 TREB-5, TREB5, XBP-1, XBP2 X-box binding protein 1 X-box-binding protein 1|tax-responsive element-binding protein 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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