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Product Info Summary
| SKU: | PB9463 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-XBP1 Antibody Picoband®
SKU/Catalog Number
PB9463
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-XBP1 Antibody Picoband® catalog # PB9463. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-XBP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9463)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human XBP1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9463 is reactive to XBP1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
28695 MW
Background of XBP1
XBP1, also known as X-box binding protein 1, is a protein which in humans is encoded by the XBP1 gene. It encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1 (S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1 (U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1 (S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9463 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: MCF-7 Whole Cell, MM231 Whole Cell, MM453 Whole Cell, SKOV Whole Cell, HELA Whole Cell
IHC: mouse lung tissue, rat lung tissue, human tonsil tissue
FCM: HepG2 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of XBP using anti-XBP antibody (PB9463).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: MCF-7 Whole Cell Lysate at 40ug,
Lane 2: MM231 Whole Cell Lysate at 40ug,
Lane 3: MM453 Whole Cell Lysate at 40ug,
Lane 4: SKOV Whole Cell Lysate at 40ug,
Lane 5: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XBP antigen affinity purified polyclonal antibody (Catalog # PB9463) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XBP at approximately 29 kDa. The expected band size for XBP is at 29 kDa.
Click image to see more details
Figure 2. IHC analysis of XBP using anti-XBP antibody (PB9463).
XBP was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-XBP Antibody (PB9463) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of XBP using anti-XBP antibody (PB9463).
XBP was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-XBP Antibody (PB9463) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of XBP using anti-XBP antibody (PB9463).
XBP was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-XBP Antibody (PB9463) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of HepG2 cells using anti-XBP1 antibody (PB9463).
Overlay histogram showing HepG2 cells stained with PB9463 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XBP1 Antibody (PB9463, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For XBP1 (Source: Uniprot.org, NCBI)
Gene Name
XBP1
Full Name
X-box-binding protein 1
Weight
28695 MW
Superfamily
bZIP family
Alternative Names
X-box-binding protein 1 ;XBP-1 ;Tax-responsive element-binding protein 5 ;TREB-5 ;X-box-binding protein 1, cytoplasmic form ;X-box-binding protein 1, luminal form ;XBP1 ;TREB5 , XBP2 ; XBP1 TREB-5, TREB5, XBP-1, XBP2 X-box binding protein 1 X-box-binding protein 1|tax-responsive element-binding protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XBP1, check out the XBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-XBP1 Antibody Picoband® (PB9463)
Hello CJ!
PB9463 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Excessive fluoride induces endoplasmic reticulum stress and interferes enamel proteinases secretion
Wang,X.,Xu,X.,Yang,Y. P.,Xin,X.,Li,Z.,Wang,Q. ... Zhang,L.(2021).Dihydromyricetin alleviates endothelial inflammatory response through IRE1α/NF-κB signaling pathway in sepsis.Archives of Medical Science.https://doi.org/10.5114/aoms/132293
Species: Human,Rat
PB9463 usage in article: APP:WB, SAMPLE:HUVECS, DILUTION:1:400
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-XBP1 Antibody Picoband®
Question
I have a question about product PB9463, anti-XBP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9463 anti-XBP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-05
Question
Please see the WB image, lot number and protocol we used for b-cell using anti-XBP1 antibody PB9463. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-13
Question
I was wanting to use your anti-XBP1 antibody for IHC-F for rat b-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat b-cell identification?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
You can see on the product datasheet, PB9463 anti-XBP1 antibody has been validated for Flow Cytometry, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat b-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-17
Question
I would like to test anti-XBP1 antibody PB9463 on rat b-cell for research purposes, then I may be interested in using anti-XBP1 antibody PB9463 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
The products we sell, including anti-XBP1 antibody PB9463, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-14
Question
I see that the anti-XBP1 antibody PB9463 works with IHC-F, what is the protocol used to produce the result images on the product page?
A. Johnson
Verified customer
Asked: 2019-10-22
Answer
You can find protocols for IHC-F on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-22
Question
Would PB9463 anti-XBP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
As indicated on the product datasheet, PB9463 anti-XBP1 antibody as been validated on IHC-F. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-06
Question
Do you have a BSA free version of anti-XBP1 antibody PB9463 available?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-XBP1 antibody PB9463 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-28
Question
We are interested in using your anti-XBP1 antibody for cellular response to glucose starvation studies. Has this antibody been tested with western blotting on mouse lung tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
Thank you for your inquiry. This PB9463 anti-XBP1 antibody is validated on mm231 whole cell lysate, mm453 whole cell lysate, skov whole cell lysate, hela whole cell lysate, mouse lung tissue, rat lung tissue, human tonsil tissue, hepg2 cells. It is guaranteed to work for Flow Cytometry, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-17
Question
We bought anti-XBP1 antibody for IHC-F on b-cell last year. I am using human, and I plan to use the antibody for IHC-P next. you antibody examining b-cell as well as body of pancreas in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
I viewed the website and datasheets of our anti-XBP1 antibody and it seems that PB9463 has been validated on human in both IHC-F and IHC-P. Thus PB9463 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-11-23
Question
We were happy with the WB result of your anti-XBP1 antibody. However we have been able to see positive staining in body of pancreas cytoplasmic form: using this antibody. Is that expected? Could you tell me where is XBP1 supposed to be expressed?
A. Banerjee
Verified customer
Asked: 2018-06-05
Answer
From literature, body of pancreas does express XBP1. Generally XBP1 expresses in endoplasmic reticulum, isoform 1: nucleus, isoform 2: nucleus, x-box-binding protein 1, cytoplasmic form:. Regarding which tissues have XBP1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 2321018
Erythroleukemia, Pubmed ID: 23186163
Ovary, and Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-06-05
Question
We have seen staining in mouse erythroleukemia. What should we do? Is anti-XBP1 antibody supposed to stain erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
From what I have seen in literature erythroleukemia does express XBP1. From what I have seen in Uniprot.org, XBP1 is expressed in body of pancreas, b-cell, ovary placenta, erythroleukemia, among other tissues. Regarding which tissues have XBP1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 2321018
Erythroleukemia, Pubmed ID: 23186163
Ovary, and Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-05-02
Question
Is this PB9463 anti-XBP1 antibody reactive to the isotypes of XBP1?
J. Thomas
Verified customer
Asked: 2017-11-29
Answer
The immunogen of PB9463 anti-XBP1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human XBP1 (59-85 aa RKRQRLTHLSPEEKALRRKLKNRVAAQ), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-29
Question
Will anti-XBP1 antibody PB9463 work for IHC-F with b-cell?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
According to the expression profile of b-cell, XBP1 is highly expressed in b-cell. So, it is likely that anti-XBP1 antibody PB9463 will work for IHC-F with b-cell.
Boster Scientific Support
Answered: 2017-10-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell using anti-XBP1 antibody PB9463. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-24
Question
Is a blocking peptide available for product anti-XBP1 antibody (PB9463)?
N. Jones
Verified customer
Asked: 2016-04-27
Answer
We do provide the blocking peptide for product anti-XBP1 antibody (PB9463). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-04-27
Question
We are currently using anti-XBP1 antibody PB9463 for human tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
J. Carter
Verified customer
Asked: 2015-04-03
Answer
The anti-XBP1 antibody (PB9463) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-03
Question
Will anti-XBP1 antibody PB9463 work on canine WB with b-cell?
P. Miller
Verified customer
Asked: 2014-07-04
Answer
Our lab technicians have not validated anti-XBP1 antibody PB9463 on canine. You can run a BLAST between canine and the immunogen sequence of anti-XBP1 antibody PB9463 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine b-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-04
