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Product Info Summary
| SKU: | EKC1609 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
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Product info
Product Name
GADD153 Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1609
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The GADD153 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor GADD153 protein expression profile in cells. The kit can be used for measuring the relative amounts of GADD153 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on GADD153.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
GADD153 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1609)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1609 is reactive to DDIT3 in Human, Mouse, Rat samples
Application Guarantee
EKC1609 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-GADD153 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from LOVO cells
Protein Target Info & Infographic
Gene/Protein Information For DDIT3 (Source: Uniprot.Org, NCBI)
Gene Name
DDIT3
Full Name
DDIT3 upstream open reading frame protein
Weight
19175 MW
Alternative Names
DNA damage-inducible transcript 3 protein;DDIT-3;C/EBP zeta;C/EBP-homologous protein;CHOP;C/EBP-homologous protein 10;CHOP-10;CCAAT/enhancer-binding protein homologous protein;Growth arrest and DNA damage-inducible protein GADD153;DDIT3;CHOP, CHOP10, GADD153; DDIT3 AltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10, CHOP10, GADD153 DNA damage inducible transcript 3 DNA damage-inducible transcript 3 protein|C/EBP zeta|CCAAT/enhancer-binding protein homologous protein|alternative DDIT3 protein|c/EBP-homologous protein 10|growth arrest and DNA damage-inducible protein GADD153
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DDIT3, check out the DDIT3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DDIT3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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