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Product Info Summary
| SKU: | A00455-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CXCR2 Antibody Picoband®
View all CXCR2/IL-8RB Antibodies
SKU/Catalog Number
A00455-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CXCR2 Antibody Picoband® catalog # A00455-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CXCR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00455-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CXCR2 recombinant protein (Position: M1-Q216).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00455-3 is reactive to CXCR2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
41-50 kDa
Calculated molecular weight
22875 MW
Background of CXCR2/IL-8RB
CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family (subfamily, chemokine). Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00455-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human COLO-320 whole cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CXCR2 using anti-CXCR2 antibody (A00455-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human COLO-320 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR2 antigen affinity purified polyclonal antibody (Catalog # A00455-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR2 at approximately 41-50 kDa. The expected band size for CXCR2 is at 41 kDa.
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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-CXCR2 antibody (A00455-3).
Overlay histogram showing THP-1 cells stained with A00455-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR2 Antibody (A00455-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CXCR2 (Source: Uniprot.org, NCBI)
Gene Name
CXCR2
Full Name
C-X-C chemokine receptor type 2
Weight
22875 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Neutrophil gelatinase-associated lipocalin;NGAL;Lipocalin-2;SV-40-induced 24P3 protein;Siderocalin LCN2;p25;Lcn2; CXCR2 CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB C-X-C motif chemokine receptor 2 C-X-C chemokine receptor type 2|CXC-R2|CXCR-2|CXCR2 gene for IL8 receptor type B|GRO/MGSA receptor|IL-8 receptor type 2|IL-8R B|chemokine (CXC) receptor 2|high affinity interleukin-8 receptor B|interleukin 8 receptor type 2|interleukin 8 receptor, beta|interleukin-8 receptor type B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCR2, check out the CXCR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CXCR2 Antibody Picoband® (A00455-3)
Hello CJ!
A00455-3 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Overexpression of GRK6 attenuates neuropathic pain via suppression of CXCR2 in rat dorsal root ganglion:
Spinal translocator protein alleviates chronic neuropathic pain behavior and modulates spinal astrocyte–neuronal function in rats with L5 spinal nerve ligation model
Chemokine CXCL1 enhances inflammatory pain and increases NMDA receptor activity and COX-2 expression in spinal cord neurons via activation of CXCR2
Chemokine receptor CXCR2 in dorsal root ganglion contributes to the maintenance of inflammatory pain
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