Key=store_id Key=entity_id, value=88838 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=A00455 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2020-03-11 06:19:29 Key=updated_at, value=2024-10-19 05:12:11 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-IL8 beta CXCR2 Antibody Key=cj_search_index Key=publication_count, value=0 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=quantity_and_stock_status Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1205 Key=yoast_content_score, value=90 Key=price, value=380.0000 Key=weight, value=1.0000 Key=meta_title, value=Anti-IL8 beta CXCR2 Antibody Key=meta_description, value=Boster Bio Anti-IL8 beta CXCR2 Antibody catalog # A00455. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. Supplied as 100ul in Liquid form antibody. Key=image, value=/a/0/a00446y341-wb-1.jpg Key=small_image, value=/a/0/a00446y341-wb-1.jpg Key=thumbnail, value=/a/0/a00446y341-wb-1.jpg Key=url_key, value=anti-il8-beta-cxcr2-antibody-a00455-boster Key=url_path, value=anti-fibroblast-activation-protein-antibody-a00422-boster Key=options_container, value=Block after Info Column Key=image_label, value=Western blot analysis of lysates from Jurkat cells treated with Paclitaxel 1uM 24h, using C-RAF (Phospho-Tyr341) Antibody. The lane on the right is blocked with the phospho peptide. Key=small_image_label, value=Western blot analysis of lysates from Jurkat cells treated with Paclitaxel 1uM 24h, using C-RAF (Phospho-Tyr341) Antibody. The lane on the right is blocked with the phospho peptide. Key=thumbnail_label, value=Western blot analysis of lysates from Jurkat cells treated with Paclitaxel 1uM 24h, using C-RAF (Phospho-Tyr341) Antibody. The lane on the right is blocked with the phospho peptide. Key=msrp_display_actual_price_type, value=Use config Key=gift_message_available, value=No Key=size, value=100ul Key=protein_name, value=C-X-C chemokine receptor type 2 Key=gene_full_name, value=C-X-C chemokine receptor type 2 Key=uniprot_id, value=P25025 Key=sensitivity Key=applications, value=ELISA, IHC, WB Key=reactivity, value=Human Key=form, value=Liquid Key=concentration, value=1 mg/ml Key=predicted_reactivity Key=source_company, value=Huafeng (order through China) HF39887, cost 100ul 650RMB Key=isotype, value=IgG Key=molecular_weight, value=40759 MW Key=sample_size_available, value=25ug for $99, contact us for details Key=related_product_skus, value=A00388-2,M00388,PROTP07992,PROTP07992-1 Key=product_category, value=Primary Antibodies Key=research_category, value=Cancer, DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling, Tumor Biomarkers Key=clonality, value=Polyclonal Key=swatch_image, value=/a/0/a00446y341-wb-1.jpg Key=rating_value, value=100 Key=review_count, value=3 Key=mp_exclude_sitemap, value=No Key=yoast_facebook_image, value=/a/0/a00446y341-wb-1.jpg Key=yoast_twitter_image, value=/a/0/a00446y341-wb-1.jpg Key=cj_related_products, value=Leukocyte Migration:A01896-1^Neutrophil Activation:A00455-1|EK0722|PROTP12850^Receptor Internalization:PA1501|A01677-1 Key=description, value=Boster Bio Anti-IL8 beta CXCR2 Antibody catalog # A00455. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. Key=short_description, value=Boster Bio Anti-IL8 beta CXCR2 Antibody catalog # A00455. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. Key=meta_keyword, value=Anti-IL8 beta CXCR2 Antibody Key=contents, value=Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Key=background Key=gene_name, value=CXCR2 Key=synonyms, value=C-X-C chemokine receptor type 2;CXC-R2;CXCR-2;CDw128b;GRO/MGSA receptor;High affinity interleukin-8 receptor B;IL-8R B;IL-8 receptor type 2;CD182;CXCR2;IL8RB;
Key=application_details, value=WB 1:500-1:2000 IHC-P 1:100-1:300 ELISA 1:20000 Key=subcellular_localization, value=Cell membrane; Multi-pass membrane protein.
Key=tissue_specificity, value=Overexpressed in gastric cancer cells and
tissues (at protein level) (PubMed:25766321).
Key=protein_function, value=Receptor for interleukin-8 which is a powerful
neutrophil chemotactic factor. Binding of IL-8 to the receptor
causes activation of neutrophils. This response is mediated via a
G-protein that activates a phosphatidylinositol-calcium second
messenger system. Binds to IL-8 with high affinity. Also binds
with high affinity to CXCL3, GRO/MGSA and NAP-2.
Key=application_notes, value=This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 88 kDa in size corresponding to FAP by western blotting in the appropriate cell lysate or extract. Key=competitor_equivalent_skus, value=sc 100149|sc 100148|sc 53383|sc 52704|sc 52351|sc 59305|sc 59306|sc 69646|sc 73355|sc 135069|sc 54538|sc 100528|sc 54539|sc 54540 Key=immunogen, value=The antiserum was produced against synthesized peptide derived from the N-terminal region of human CXCR2. AA range:1-50 Key=purification, value=The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Key=storage, value=Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-IL8 beta CXCR2 Antibody
CXCR2/IL-8RB antibody
Boster Bio Anti-IL8 beta CXCR2 Antibody catalog # A00455. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Boster Bio Anti-IL8 beta CXCR2 Antibody catalog # A00455. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL8 beta CXCR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00455)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the N-terminal region of human CXCR2. AA range:1-50
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00455 is reactive to CXCR2 in Human
Reconstitution
Calculated molecular weight
40759 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000 IHC-P 1:100-1:300 ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of CXCR2 using Anti-IL8 beta CXCR2 Antibody (A00455).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain
Click image to see more details
Figure 3. Immunohistochemistry validation of CXCR2 using Anti-IL8 beta CXCR2 Antibody (A00455).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain
Click image to see more details
Figure 1. Western blotting validation for Anti-IL8 beta CXCR2 Antibody A00455
Western Blot (WB) analysis of HuvEc cells using IL-8Rbeta polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Gene/Protein Information For CXCR2 (Source: Uniprot.org, NCBI)
Gene Name
CXCR2
Full Name
C-X-C chemokine receptor type 2
Weight
40759 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-X-C chemokine receptor type 2;CXC-R2;CXCR-2;CDw128b;GRO/MGSA receptor;High affinity interleukin-8 receptor B;IL-8R B;IL-8 receptor type 2;CD182;CXCR2;IL8RB;
CXCR2 CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB C-X-C motif chemokine receptor 2 C-X-C chemokine receptor type 2|CXC-R2|CXCR-2|CXCR2 gene for IL8 receptor type B|GRO/MGSA receptor|IL-8 receptor type 2|IL-8R B|chemokine (CXC) receptor 2|high affinity interleukin-8 receptor B|interleukin 8 receptor type 2|interleukin 8 receptor, beta|interleukin-8 receptor type B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on CXCR2, check out the CXCR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$380.00
Troubleshooting
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.