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Product Info Summary
| SKU: | EK0823 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine®
SKU/Catalog Number
EK0823
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cxcl5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0823)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A38-Q130
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0823 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0823 is reactive to Cxcl5 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0823 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL5
C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0823-CAP | Anti-Rat Cxcl5 Pre-coated 96-well strip microplate | 1 |
| EK0823-ST | Rat Cxcl5 Standard | 2 vials, 10 ng/tube |
| EK0823-DA | Rat Cxcl5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.008 | 0.038 | 0.093 | 0.195 | 0.381 | 0.793 | 1.456 | 2.357 |
Data Example Images
Click image to see more details
Rat CXCL5 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CXCL5, Dilution ratio of 1:100, concentration in serum and plasma is around 20 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 38 | 170 | 439 | 39 | 159 | 411 |
| Standard deviation | 1.52 | 7.14 | 32.04 | 1.98 | 7.95 | 30.82 |
| CV (%) | 4% | 4.2% | 7.3% | 7.5%% | 5% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL5 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 38 | 36 | 37 | 36 | 36 | 0.82 | 2.2% |
| Sample 2 | 170 | 164 | 174 | 167 | 168 | 3.69 | 2.1% |
| Sample 3 | 439 | 513 | 501 | 456 | 477 | 30.64 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For Cxcl5 (Source: Uniprot.Org, NCBI)
Gene Name
Cxcl5
Full Name
C-X-C motif chemokine 5
Weight
14263 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
C-X-C motif chemokine 5;Cytokine LIX;Small-inducible cytokine B5;Cxcl5;Scyb5; CXCL5 ENA-78, SCYB5 C-X-C motif chemokine ligand 5 C-X-C motif chemokine 5|chemokine (C-X-C motif) ligand 5|epithelial-derived neutrophil-activating protein 78|neutrophil-activating peptide ENA-78|neutrophil-activating protein 78|small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cxcl5, check out the Cxcl5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cxcl5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine® (EK0823)
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No publications found for EK0823
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine®
Question
Does EK0823 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine® (EK0823) doesn't contain native human biological materials. doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
What is the gene ID of EK0823?
Verified customer
Asked: 2021-02-22
Answer
The gene ID of the Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine™ (EK0823) is 60665, Cxcl6 C-X-C motif chemokine ligand 6 [ Rattus norvegicus (Norway rat) ].
Boster Scientific Support
Answered: 2021-02-23
Question
Q: can I use citrate plasma as samples in Rat CXCL5 Picokine® ELISA Kit (Catalog # EK0823)?
Verified Customer
Verified customer
Asked: 2020-10-10
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CXCL5 causing disruption of its protein structure. CXCL5 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CXCL5 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-10
Question
Q: how can I thaw whole blood sample for CXCL5 ELISA after freezing?
C. Williams
Verified customer
Asked: 2020-09-01
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CXCL5 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-09-01
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Evans
Verified customer
Asked: 2020-07-31
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-07-31
Question
I am interested in your EK0823 Rat CXCL5/LIX/ENA-78 PicoKine
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
We haven't detected CXCL6 samples with EK0823. According to the sequence homology, EK0823 will most likely react with CXCL6. The standard of the kit is Cxcl5.
Boster Scientific Support
Answered: 2019-10-11
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CXCL5 using the EK0823 Rat CXCL5 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-10-08
Question
Q: if the enzyme conjugated CXCL5 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CXCL5 antigen?
M. Smith
Verified customer
Asked: 2018-04-02
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-04-02
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-10-17
Question
Q: Can CXCL5 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
D. Moore
Verified customer
Asked: 2016-02-05
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-02-05
