Click image to see more details
Product Info Summary
| SKU: | A01896-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CCR1 Antibody Picoband®
SKU/Catalog Number
A01896-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCR1 Antibody Picoband® catalog # A01896-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01896-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CCR1 recombinant protein (Position: A168-T339). Mouse CCR1 shares 76.5% amino acid (aa) sequence identity with human CCR1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01896-1 is reactive to Ccr1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
40895 MW
Background of CCR1
C-C chemokine receptor type 1 is a protein that in humans is encoded by the CCR1 gene. This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01896-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Mouse
Positive Control
WB: rat PC-12 whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CCR1 using anti-CCR1 antibody (A01896-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat PC-12 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR1 antigen affinity purified polyclonal antibody (Catalog # A01896-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR1 at approximately 48KD. The expected band size for CCR1 is at 41KD.
Protein Target Info & Infographic
Gene/Protein Information For Ccr1 (Source: Uniprot.org, NCBI)
Gene Name
Ccr1
Full Name
C-C chemokine receptor type 1
Weight
40895 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-C chemokine receptor type 1;C-C CKR-1;CC-CKR-1;CCR-1;CCR1;Macrophage inflammatory protein 1-alpha receptor;MIP-1alpha-R;RANTES-R;CD191;Ccr1;Cmkbr1; CCR1 CD191, CKR-1, CKR1, CMKBR1, HM145, MIP1aR, SCYAR1 C-C motif chemokine receptor 1 C-C chemokine receptor type 1|C-C CKR-1|CC-CKR-1|CCR-1|LD78 receptor|MIP-1alpha-R|RANTES receptor|RANTES-R|chemokine (C-C motif) receptor 1|macrophage inflammatory protein 1-alpha receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ccr1, check out the Ccr1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ccr1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCR1 Antibody Picoband® (A01896-1)
Hello CJ!
A01896-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CCR1 Antibody Picoband®
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-CCR1 antibody A01896-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-01
Question
We have seen staining in rat leukocyte. Do you have any suggestions? Is anti-CCR1 antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
According to literature leukocyte does express CCR1. According to Uniprot.org, CCR1 is expressed in leukocyte, monocyte, blood, among other tissues. Regarding which tissues have CCR1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Monocyte, Pubmed ID: 7505609
Boster Scientific Support
Answered: 2020-04-01
Question
Is a blocking peptide available for product anti-CCR1 antibody (A01896-1)?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
We do provide the blocking peptide for product anti-CCR1 antibody (A01896-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-12
Question
Will A01896-1 anti-CCR1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
It shows on the product datasheet, A01896-1 anti-CCR1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-08
Question
Here is the WB image, lot number and protocol we used for leukocyte using anti-CCR1 antibody A01896-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-31
Question
We are currently using anti-CCR1 antibody A01896-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on monkey tissues as well?
K. Jha
Verified customer
Asked: 2019-11-12
Answer
The anti-CCR1 antibody (A01896-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-12
Question
Is this A01896-1 anti-CCR1 antibody reactive to the isotypes of CCR1?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
The immunogen of A01896-1 anti-CCR1 antibody is E. coli-derived mouse CCR1 recombinant protein (Position: A168-T339). Mouse CCR1 shares 76.5% amino acid (aa) sequence identity with human CCR1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-09
Question
Our lab were well pleased with the WB result of your anti-CCR1 antibody. However we have seen positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is CCR1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
From what I have seen in literature, blood does express CCR1. Generally CCR1 expresses in cell membrane. Regarding which tissues have CCR1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Monocyte, Pubmed ID: 7505609
Boster Scientific Support
Answered: 2019-08-02
Question
I am looking for to test anti-CCR1 antibody A01896-1 on mouse leukocyte for research purposes, then I may be interested in using anti-CCR1 antibody A01896-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
The products we sell, including anti-CCR1 antibody A01896-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-27
Question
I have a question about product A01896-1, anti-CCR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01896-1 anti-CCR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-16
Question
I was wanting to use your anti-CCR1 antibody for WB for mouse leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse leukocyte identification?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
It shows on the product datasheet, A01896-1 anti-CCR1 antibody has been tested for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-06
Question
Does anti-CCR1 antibody A01896-1 work on monkey WB with blood?
V. Singh
Verified customer
Asked: 2018-11-21
Answer
Our lab technicians have not tested anti-CCR1 antibody A01896-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-CCR1 antibody A01896-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-21
Question
Is there a BSA free version of anti-CCR1 antibody A01896-1 available?
P. Evans
Verified customer
Asked: 2014-06-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CCR1 antibody A01896-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-06-04
Question
Will anti-CCR1 antibody A01896-1 work for WB with leukocyte?
A. Mangal
Verified customer
Asked: 2014-02-28
Answer
According to the expression profile of leukocyte, CCR1 is highly expressed in leukocyte. So, it is likely that anti-CCR1 antibody A01896-1 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2014-02-28
Question
I see that the anti-CCR1 antibody A01896-1 works with WB, what is the protocol used to produce the result images on the product page?
A. Taylor
Verified customer
Asked: 2013-01-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-01-11
