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Key=meta_title, value=Anti-CCR1 Antibody Picoband | Bosterbio A01896-1
Key=meta_description, value=Boster Bio Anti-CCR1 Antibody Picoband® catalog # A01896-1. Tested in WB applications. This antibody reacts with Mouse, Rat. Supplied as 100ug/vial in Lyophilized form antibody.
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Key=image_label, value=Figure 1. Western blot analysis of CCR1 using anti-CCR1 antibody (A01896-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of
Key=small_image_label, value=Figure 1. Western blot analysis of CCR1 using anti-CCR1 antibody (A01896-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of
Key=thumbnail_label, value=Figure 1. Western blot analysis of CCR1 using anti-CCR1 antibody (A01896-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of
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Key=size, value=100 μg/vial
Key=gene_full_name, value=C-C chemokine receptor type 1
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Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Key=applications, value=WB
Key=reactivity, value=Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins.
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Key=predicted_reactivity
Key=source_company, value=Boster A01896-1
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=40895 MW
Key=sample_size_available, value=30ug for $99, contact us for details
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Key=product_category, value=Primary Antibodies
Key=research_category, value=Chemokines, Immunology, Innate Immunity
Key=clonality, value=Polyclonal
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Key=cj_related_products, value=Leukocyte Migration:EK1745^Osteoclast Differentiation:A01064-2|FEK1281|FEK0830|A01064-3^Pertussis:EK1946
Key=description, value=Boster Bio Anti-CCR1 Antibody Picoband® catalog # A01896-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-CCR1 Antibody Picoband® catalog # A01896-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-CCR1 Antibody Picoband®
Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=C-C chemokine receptor type 1 is a protein that in humans is encoded by the CCR1 gene. This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite.
Key=gene_name, value=Ccr1
Key=synonyms, value=C-C chemokine receptor type 1;C-C CKR-1;CC-CKR-1;CCR-1;CCR1;Macrophage inflammatory protein 1-alpha receptor;MIP-1alpha-R;RANTES-R;CD191;Ccr1;Cmkbr1;
Key=application_details, value=Western blot, 0.1-0.5μg/ml, Rat, Mouse

Key=subcellular_localization, value= Cell membrane; Multi-pass membrane protein.
Key=tissue_specificity, value= Detected in the heart, spleen, lung, peritoneal exudate cells and leukocytes.
Key=protein_function, value= Receptor for a C-C type chemokine. Binds to MIP-1-alpha, RANTES, and less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.
Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 1382|sc 1381|sc 33203|sc 166911|sc 166942|sc 393899|sc 1383|sc 365691|sc 6125|sc 31567|sc 7934
Key=immunogen, value=E. coli-derived mouse CCR1 recombinant protein (Position: A168-T339). Mouse CCR1 shares 76.5% amino acid (aa) sequence identity with human CCR1.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=48 kDa
Key=custom_attribute_2, value=WB: rat PC-12 whole Cell
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Anti-CCR1 Antibody Picoband®

CCR1 antibody

Boster Bio Anti-CCR1 Antibody Picoband® catalog # A01896-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: A01896-1
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-CCR1 Antibody Picoband®

View all CCR1 Antibodies

SKU/Catalog Number

A01896-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CCR1 Antibody Picoband® catalog # A01896-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CCR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01896-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse CCR1 recombinant protein (Position: A168-T339). Mouse CCR1 shares 76.5% amino acid (aa) sequence identity with human CCR1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01896-1 is reactive to Ccr1 in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

48 kDa

Calculated molecular weight

40895 MW

Background of CCR1

C-C chemokine receptor type 1 is a protein that in humans is encoded by the CCR1 gene. This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A01896-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Mouse

Positive Control

WB: rat PC-12 whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For Ccr1 (Source: Uniprot.org, NCBI)

Gene Name

Ccr1

Full Name

C-C chemokine receptor type 1

Weight

40895 MW

Superfamily

G-protein coupled receptor 1 family

Alternative Names

C-C chemokine receptor type 1;C-C CKR-1;CC-CKR-1;CCR-1;CCR1;Macrophage inflammatory protein 1-alpha receptor;MIP-1alpha-R;RANTES-R;CD191;Ccr1;Cmkbr1; CCR1 CD191, CKR-1, CKR1, CMKBR1, HM145, MIP1aR, SCYAR1 C-C motif chemokine receptor 1 C-C chemokine receptor type 1|C-C CKR-1|CC-CKR-1|CCR-1|LD78 receptor|MIP-1alpha-R|RANTES receptor|RANTES-R|chemokine (C-C motif) receptor 1|macrophage inflammatory protein 1-alpha receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Ccr1, check out the Ccr1 Infographic

Ccr1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ccr1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01896-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis

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15 Customer Q&As for Anti-CCR1 Antibody Picoband®

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-CCR1 antibody A01896-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-05-01

Question

We have seen staining in rat leukocyte. Do you have any suggestions? Is anti-CCR1 antibody supposed to stain leukocyte positively?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

According to literature leukocyte does express CCR1. According to Uniprot.org, CCR1 is expressed in leukocyte, monocyte, blood, among other tissues. Regarding which tissues have CCR1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Monocyte, Pubmed ID: 7505609

Boster Scientific Support

Answered: 2020-04-01

Question

Is a blocking peptide available for product anti-CCR1 antibody (A01896-1)?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

We do provide the blocking peptide for product anti-CCR1 antibody (A01896-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-12

Question

Will A01896-1 anti-CCR1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

It shows on the product datasheet, A01896-1 anti-CCR1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-08

Question

Here is the WB image, lot number and protocol we used for leukocyte using anti-CCR1 antibody A01896-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-31

Question

We are currently using anti-CCR1 antibody A01896-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on monkey tissues as well?

K. Jha

Verified customer

Asked: 2019-11-12

Answer

The anti-CCR1 antibody (A01896-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-12

Question

Is this A01896-1 anti-CCR1 antibody reactive to the isotypes of CCR1?

Verified Customer

Verified customer

Asked: 2019-09-09

Answer

The immunogen of A01896-1 anti-CCR1 antibody is E. coli-derived mouse CCR1 recombinant protein (Position: A168-T339). Mouse CCR1 shares 76.5% amino acid (aa) sequence identity with human CCR1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-09

Question

Our lab were well pleased with the WB result of your anti-CCR1 antibody. However we have seen positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is CCR1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

From what I have seen in literature, blood does express CCR1. Generally CCR1 expresses in cell membrane. Regarding which tissues have CCR1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Monocyte, Pubmed ID: 7505609

Boster Scientific Support

Answered: 2019-08-02

Question

I am looking for to test anti-CCR1 antibody A01896-1 on mouse leukocyte for research purposes, then I may be interested in using anti-CCR1 antibody A01896-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-27

Answer

The products we sell, including anti-CCR1 antibody A01896-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-27

Question

I have a question about product A01896-1, anti-CCR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01896-1 anti-CCR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-16

Question

I was wanting to use your anti-CCR1 antibody for WB for mouse leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse leukocyte identification?

Verified Customer

Verified customer

Asked: 2019-03-06

Answer

It shows on the product datasheet, A01896-1 anti-CCR1 antibody has been tested for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-06

Question

Does anti-CCR1 antibody A01896-1 work on monkey WB with blood?

V. Singh

Verified customer

Asked: 2018-11-21

Answer

Our lab technicians have not tested anti-CCR1 antibody A01896-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-CCR1 antibody A01896-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey blood in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-21

Question

Is there a BSA free version of anti-CCR1 antibody A01896-1 available?

P. Evans

Verified customer

Asked: 2014-06-04

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CCR1 antibody A01896-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-06-04

Question

Will anti-CCR1 antibody A01896-1 work for WB with leukocyte?

A. Mangal

Verified customer

Asked: 2014-02-28

Answer

According to the expression profile of leukocyte, CCR1 is highly expressed in leukocyte. So, it is likely that anti-CCR1 antibody A01896-1 will work for WB with leukocyte.

Boster Scientific Support

Answered: 2014-02-28

Question

I see that the anti-CCR1 antibody A01896-1 works with WB, what is the protocol used to produce the result images on the product page?

A. Taylor

Verified customer

Asked: 2013-01-11

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-01-11

Order DetailsPrice
A01896-1

100μg

$370
A01896-1-10ug

10μg sample (liquid)

$99
A01896-1-Biotin

100 μg Biotin conjugated

$570
A01896-1-Cy3

100 μg Cy3 conjugated

$570
A01896-1-Dylight488

100 μg Dylight488 conjugated

$570
A01896-1-Dylight550

100 μg Dylight550 conjugated

$570
A01896-1-Dylight594

100 μg Dylight594 conjugated

$570
A01896-1-FITC

100 μg FITC conjugated

$570
A01896-1-HRP

100 μg HRP conjugated

$570
A01896-1-APC

100 μg APC conjugated

$670
A01896-1-PE

100 μg PE conjugated

$670
A01896-1-iFluor647

100 μg iFluor647 conjugated

$670
A01896-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A01896-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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