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Product Info Summary
| SKU: | A00533 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GRO alpha/Cxcl1 Antibody Picoband®
View all CXCL1/GRO alpha/KC/CINC-1 Antibodies
SKU/Catalog Number
A00533
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GRO alpha/Cxcl1 Antibody Picoband® catalog # A00533. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRO alpha/Cxcl1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00533)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat GRO alpha recombinant protein (Position: A25-K96).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00533 is reactive to Cxcl1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated molecular weight
11.301kDa
Background of CXCL1/GRO alpha/KC/CINC-1
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00533 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GRO alpha using anti-GRO alpha antibody (A00533).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant rat CXCL1 protein 10 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRO alpha antigen affinity purified polyclonal antibody (Catalog # A00533) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRO alpha at approximately 11 kDa.
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Figure 2. Sandwich ELISA - Recombinant rat GRO alpha/Cxcl1 protein standard curve.
Use in combination with reagents from Rat GRO alpha/Cxcl1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0724).
Protein Target Info & Infographic
Gene/Protein Information For Cxcl1 (Source: Uniprot.org, NCBI)
Gene Name
Cxcl1
Full Name
Growth-regulated alpha protein
Weight
11.301kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
Growth-regulated alpha protein; C-X-C motif chemokine 1; Cytokine-induced neutrophil chemoattractant 1; CINC-1; Platelet-derived growth factor-inducible protein KC; Cxcl1; Cinc1; Gro; Scyb1 CXCL1 FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1 C-X-C motif chemokine ligand 1 growth-regulated alpha protein|C-X-C motif chemokine 1|GRO-alpha(1-73)|GRO1 oncogene (melanoma growth stimulating activity, alpha)|GRO1 oncogene (melanoma growth-stimulating activity)|MGSA alpha|chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)|fibroblast secretory protein|melanoma growth stimulating activity, alpha|melanoma growth stimulatory activity alpha|neutrophil-activating protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cxcl1, check out the Cxcl1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cxcl1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GRO alpha/Cxcl1 Antibody Picoband® (A00533)
Hello CJ!
A00533 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of Tff3 Deficiency and ER Stress in the Liver
Activation of Mast Cells Promote Plasmodium berghei ANKA Infection in Murine Model
Activated spinal astrocytes contribute to the later phase of carrageenan-induced prostatitis pain
HAb18G/CD147 Functions in Invasion and Metastasis of Hepatocellular Carcinoma
C-X-C motif chemokine ligand 1 and its receptor C-X-C motif chemokine receptor 2 in trigeminal ganglion contribute to nerve injury-induced orofacial mechanical allodynia
Levo-corydalmine alleviates vincristine-induced neuropathic pain in mice by inhibiting an NF-kappa B-dependent CXCL1/CXCR2 signaling pathway
Effect of curcumin on IL-17A mediated pulmonary AMPK kinase/cyclooxygenase-2 expressions via activation of NFκB in bleomycin-induced acute lung injury in vivo
The neuroprotection of hyperbaric oxygen therapy against traumatic brain injury via NF-κB/MAPKs-CXCL1 signaling pathways
Connexin-43 induces chemokine release from spinal cord astrocytes to maintain late-phase neuropathic pain in mice
Species: Mouse
Anqi Xia,Huan Huang,Wenjun You et al.The neuroprotection of hyperbaric oxygen therapy against traumatic brain injury via NF-κB/MAPKs-CXCL1 signaling pathway, 28 December 2020, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.
Species: Rat
A00533 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:50
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