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Product Info Summary
| SKU: | PA1029 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CXCR2 Antibody Picoband®
View all CXCR2/IL-8RB Antibodies
SKU/Catalog Number
PA1029
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CXCR2 Antibody catalog # PA1029. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1029)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CXCR2, different from the related mouse sequence by six amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1029 is reactive to CXCR2 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
40759 MW
Background of CXCR2/IL-8RB
CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family (subfamily, chemokine). Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1029 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat lung tissue, human COLO320 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CXCR2 using anti-CXCR2 antibody (PA1029).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: human COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR2 antigen affinity purified polyclonal antibody (Catalog # PA1029) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR2 at approximately 45 kDa. The expected band size for CXCR2 is at 41 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CXCR2 (Source: Uniprot.org, NCBI)
Gene Name
CXCR2
Full Name
C-X-C chemokine receptor type 2
Weight
40759 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-X-C chemokine receptor type 2;CXC-R2;CXCR-2;CDw128b;GRO/MGSA receptor;High affinity interleukin-8 receptor B;IL-8R B;IL-8 receptor type 2;CD182;CXCR2;IL8RB; CXCR2 CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB C-X-C motif chemokine receptor 2 C-X-C chemokine receptor type 2|CXC-R2|CXCR-2|CXCR2 gene for IL8 receptor type B|GRO/MGSA receptor|IL-8 receptor type 2|IL-8R B|chemokine (CXC) receptor 2|high affinity interleukin-8 receptor B|interleukin 8 receptor type 2|interleukin 8 receptor, beta|interleukin-8 receptor type B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCR2, check out the CXCR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CXCR2 Antibody Picoband® (PA1029)
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Customer Q&As
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4 Customer Q&As for Anti-CXCR2 Antibody Picoband®
Question
We are currently using anti-CXCR2 antibody PA1029 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
D. Banerjee
Verified customer
Asked: 2020-05-01
Answer
The anti-CXCR2 antibody (PA1029) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-01
Question
We have seen staining in mouse brain. Do you have any suggestions? Is anti-CXCR2 antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
Based on literature brain does express CXCR2. Based on Uniprot.org, CXCR2 is expressed in blood, placenta, brain, among other tissues. Regarding which tissues have CXCR2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Placenta, Pubmed ID: 7929358
Boster Scientific Support
Answered: 2018-04-25
Question
I am looking for using your anti-CXCR2 antibody for positive regulation of angiogenesis studies. Has this antibody been tested with western blotting on kidney tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
I appreciate your inquiry. This PA1029 anti-CXCR2 antibody is tested on human rectal cancer tissue lysate, rat spleen tissue, kidney tissue, brain tissue, mouse testis tissue, hepa whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-01-26
Question
My team were well pleased with the WB result of your anti-CXCR2 antibody. However we have seen positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is CXCR2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
According to literature, placenta does express CXCR2. Generally CXCR2 expresses in cell membrane. Regarding which tissues have CXCR2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Placenta, Pubmed ID: 7929358
Boster Scientific Support
Answered: 2017-12-14
