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Product Info Summary
| SKU: | A00455-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC |
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Product info
Product Name
Anti-CXCR2 Antibody
View all CXCR2/IL-8RB Antibodies
SKU/Catalog Number
A00455-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CXCR2 Antibody catalog # A00455-1. Tested in Flow Cytometry, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00455-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR2, which shares 52.4% amino acid (aa) sequence identity with rat CXCR2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00455-1 is reactive to CXCR2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
68 kDa
Calculated molecular weight
22875 MW
Background of CXCR2/IL-8RB
CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family (subfamily, chemokine). Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00455-1 is guaranteed for Flow Cytometry, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
IHC: human spleen tissue
FCM: human PBMC cell
Validation Images & Assay Conditions
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Figure 2. Flow Cytometry analysis of human PBMC cells using anti-CXCR2 antibody (A00455-1).
Overlay histogram showing human PBMC cells stained with A00455-1 (Blue line). The cells fixed with 4% paraformaldehyde and were blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR2 Antibody (A00455-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. IHC analysis of CXCR2 using anti-CXCR2 antibody (A00455-1).
CXCR2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCR2 Antibody (A00455-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CXCR2 (Source: Uniprot.org, NCBI)
Gene Name
CXCR2
Full Name
C-X-C chemokine receptor type 2
Weight
22875 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Neutrophil gelatinase-associated lipocalin;NGAL;Lipocalin-2;SV-40-induced 24P3 protein;Siderocalin LCN2;p25;Lcn2; CXCR2 CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB C-X-C motif chemokine receptor 2 C-X-C chemokine receptor type 2|CXC-R2|CXCR-2|CXCR2 gene for IL8 receptor type B|GRO/MGSA receptor|IL-8 receptor type 2|IL-8R B|chemokine (CXC) receptor 2|high affinity interleukin-8 receptor B|interleukin 8 receptor type 2|interleukin 8 receptor, beta|interleukin-8 receptor type B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCR2, check out the CXCR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CXCR2 Antibody (A00455-1)
Hello CJ!
A00455-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cui D,Zhao Y,Xu J.Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38 pathways.Cancer Biol Ther.2019;20(5):608-616.doi:10.1080/15384047.2018.1539289.Epub 2018 Nov 7.PMID:30404567;PMCID:PMC6606038.
Species: Human,Mouse
A00455-1 usage in article: APP:WB, SAMPLE:PTC CELL, DILUTION:1:200
Anqi Xia,Huan Huang,Wenjun You et al.The neuroprotection of hyperbaric oxygen therapy against traumatic brain injury via NF-κB/MAPKs-CXCL1 signaling pathway, 28 December 2020, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.
Species: Rat
A00455-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:50
Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38 pathways Dong Cui 1, Yongfu Zhao 1, Jingchao Xu 1 Cancer Biol Ther. 2019;20(5):608-616. doi:
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