Anti-CREB (phospho-S142) CREB1 Antibody

Product Name

Anti-CREB (phospho-S142) CREB1 Antibody

View all CREB Antibodies

SKU/Catalog Number

A00577S142

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CREB (phospho-S142) CREB1 Antibody catalog # A00577S142. Tested in IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CREB (phospho-S142) CREB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00577S142)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human CREB-1 around the phosphorylation site of S121.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00577S142 is reactive to CREB1 in Human, Mouse, Rat

Applications

A00577S142 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

36688 MW

Background of CREB

Transcriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer. Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes By similarity. Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions.

Nagamine K., Nat. Genet. 17:393-398(1997).
Aaltonen J., Nat. Genet. 17:399-403(1997).
Hattori M., Nature 405:311-319(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 1. Immunohistochemistry validation of CREB1 using Anti-CREB (phospho-S142) CREB1 Antibody (A00577S142).

Immunohistochemistry (IHC) analyzes of p-CREB (S142) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
For more protocol information of IHC

Gene/Protein Information For CREB1 (Source: Uniprot.org, NCBI)

Alternative Names

active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; pCREB; transactivator protein CREB1 CREB, CREB-1 cAMP responsive element binding protein 1 cyclic AMP-responsive element-binding protein 1|active transcription factor CREB|cAMP-response element-binding protein-1|cyclic adenosine 3,5-monophosphate response element binding protein|cyclic adenosine 3,5-monophosphate response element-binding protein CREB|transactivator protein

For more info on CREB1, check out the CREB1 Infographic

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In this infographic, you will see the following information for CREB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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