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Product Info Summary
| SKU: | A04946 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PREB Antibody Picoband®
SKU/Catalog Number
A04946
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PREB Antibody Picoband® catalog # A04946. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PREB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04946)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PREB recombinant protein (Position: K42-N236). Human PREB shares 87.7% amino acid (aa) sequence identity with both mouse and rat PREB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04946 is reactive to PREB in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
45468 MW
Background of PREB
Prolactin regulatory element-binding protein is a protein that in humans is encoded by the PREB gene. This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04946 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human placenta tissue, human A549 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PREB using anti-PREB antibody (A04946).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human placenta tissue lysate,
Lane 3: human A549 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PREB antigen affinity purified polyclonal antibody (Catalog # A04946) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PREB at approximately 45KD. The expected band size for PREB is at 45KD.
Protein Target Info & Infographic
Gene/Protein Information For PREB (Source: Uniprot.org, NCBI)
Gene Name
PREB
Full Name
Prolactin regulatory element-binding protein
Weight
45468 MW
Alternative Names
Prolactin regulatory element-binding protein;Mammalian guanine nucleotide exchange factor mSec12;PREB;SEC12; PREB SEC12 prolactin regulatory element binding prolactin regulatory element-binding protein|mammalian guanine nucleotide exchange factor mSec12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PREB, check out the PREB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PREB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PREB Antibody Picoband® (A04946)
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No publications found for A04946
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Customer Q&As
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13 Customer Q&As for Anti-PREB Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-PREB antibody A04946. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-26
Question
Here is the WB image, lot number and protocol we used for liver using anti-PREB antibody A04946. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-28
Question
Do you have a BSA free version of anti-PREB antibody A04946 available?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PREB antibody A04946 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-30
Question
Will A04946 anti-PREB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
You can see on the product datasheet, A04946 anti-PREB antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-13
Question
Does anti-PREB antibody A04946 work for WB with liver?
V. Dhar
Verified customer
Asked: 2018-05-15
Answer
According to the expression profile of liver, PREB is highly expressed in liver. So, it is likely that anti-PREB antibody A04946 will work for WB with liver.
Boster Scientific Support
Answered: 2018-05-15
Question
Is this A04946 anti-PREB antibody reactive to the isotypes of PREB?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
The immunogen of A04946 anti-PREB antibody is E. coli-derived human PREB recombinant protein (Position: K42-N236). Human PREB shares 87.7% amino acid (aa) sequence identity with both mouse and rat PREB. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-30
Question
I have a question about product A04946, anti-PREB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-03-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04946 anti-PREB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-02
Question
We need to test anti-PREB antibody A04946 on human liver for research purposes, then I may be interested in using anti-PREB antibody A04946 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Bhatt
Verified customer
Asked: 2017-10-20
Answer
The products we sell, including anti-PREB antibody A04946, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-20
Question
I was wanting to use your anti-PREB antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2017-10-16
Answer
It shows on the product datasheet, A04946 anti-PREB antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-16
Question
We are currently using anti-PREB antibody A04946 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
G. Rodriguez
Verified customer
Asked: 2015-04-15
Answer
The anti-PREB antibody (A04946) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-15
Question
I see that the anti-PREB antibody A04946 works with WB, what is the protocol used to produce the result images on the product page?
M. Thomas
Verified customer
Asked: 2014-12-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-12-05
Question
Would anti-PREB antibody A04946 work on zebrafish WB with liver?
J. Li
Verified customer
Asked: 2013-04-22
Answer
Our lab technicians have not tested anti-PREB antibody A04946 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PREB antibody A04946 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-04-22
Question
Is a blocking peptide available for product anti-PREB antibody (A04946)?
V. Anderson
Verified customer
Asked: 2013-01-31
Answer
We do provide the blocking peptide for product anti-PREB antibody (A04946). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-01-31
