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Product Info Summary
| SKU: | P00577-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
P00577-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal Antibody catalog # P00577-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00577-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Creb (S133)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00577-1 is reactive to CREB1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
185 kDa
Calculated molecular weight
36688 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00577-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
FC 1:40
Positive Control
IHC: human gastric carcinoma tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-Creb(Ser133) expression in HeLa cell lysates treated with Calyculin A.
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Immunohistochemical analysis of paraffin-embedded human gastric carcinoma, using Phospho-Creb (S133) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CREB1 (Source: Uniprot.org, NCBI)
Gene Name
CREB1
Full Name
Cyclic AMP-responsive element-binding protein 1
Weight
36688 MW
Superfamily
bZIP family
Alternative Names
Cyclic AMP-responsive element-binding protein 1;CREB-1;cAMP-responsive element-binding protein 1;CREB1; CREB1 CREB, CREB-1 cAMP responsive element binding protein 1 cyclic AMP-responsive element-binding protein 1|active transcription factor CREB|cAMP-response element-binding protein-1|cyclic adenosine 3,5-monophosphate response element binding protein|cyclic adenosine 3,5-monophosphate response element-binding protein CREB|transactivator protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CREB1, check out the CREB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CREB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal Antibody (P00577-1)
Hello CJ!
P00577-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
LncRNA DQ786243 affects Treg related CREB and Foxp3 expression in Crohn?s disease
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal Antibody
Question
Our team were satisfied with the WB result of your anti-Phospho-Creb (S133) Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma erythroleukemia nucleus using this antibody. Is that expected? Could you tell me where is CREB1 supposed to be expressed?
B. Taylor
Verified customer
Asked: 2018-03-23
Answer
Based on literature, cervix carcinoma erythroleukemia does express CREB1. Generally CREB1 expresses in nucleus. Regarding which tissues have CREB1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 15579595
Boster Scientific Support
Answered: 2018-03-23
Question
We have seen staining in rat testis. Any tips? Is anti-Phospho-Creb (S133) Rabbit Monoclonal antibody supposed to stain testis positively?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
According to literature testis does express CREB1. According to Uniprot.org, CREB1 is expressed in lung, testis, eye, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have CREB1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 15579595
Boster Scientific Support
Answered: 2017-12-01
Question
We bought anti-Phospho-Creb (S133) Rabbit Monoclonal antibody for Flow Cytometry on cervix carcinoma in the past. I am using mouse, and We intend to use the antibody for ICC next. My lab would like examining cervix carcinoma as well as embryonic kidney in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
S. Jha
Verified customer
Asked: 2015-06-16
Answer
I viewed the website and datasheets of our anti-Phospho-Creb (S133) Rabbit Monoclonal antibody and I see that P00577-1 has been tested on mouse in both Flow Cytometry and ICC. Thus P00577-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-06-16
Question
We are currently using anti-Phospho-Creb (S133) Rabbit Monoclonal antibody P00577-1 for rat tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
V. Thomas
Verified customer
Asked: 2014-10-22
Answer
The anti-Phospho-Creb (S133) Rabbit Monoclonal antibody (P00577-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-22
