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Product Info Summary
| SKU: | A00577S133-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CREB (Phospho-S133) CREB1 Antibody
SKU/Catalog Number
A00577S133-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CREB (Phospho-S133) CREB1 Antibody catalog # A00577S133-1. Tested in WB,ICC,IF,IHC,IP,Flow Cytometry applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CREB (Phospho-S133) CREB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00577S133-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser133 of human Creb.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00577S133-1 is reactive to CREB1 in Human, Mouse
Reconstitution
Calculated molecular weight
36688 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00577S133-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:50-1:100
IHC: 1:50-1:200
FC: 1:50-1:100
IP: 1:10-1:50
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of CREB1 using Anti-CREB (Phospho-S133) CREB1 Antibody (A00577S133-1).
ICC staining Phospho-Creb (S133) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-CREB (Phospho-S133) CREB1 Antibody A00577S133-1
Western blot analysis of Phospho-Creb (S133) on human colon tissue lysates using anti-Phospho-Creb (S133) antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CREB1 (Source: Uniprot.org, NCBI)
Gene Name
CREB1
Full Name
Cyclic AMP-responsive element-binding protein 1
Weight
36688 MW
Superfamily
bZIP family
Alternative Names
Cyclic AMP-responsive element-binding protein 1;CREB-1;cAMP-responsive element-binding protein 1;CREB1; CREB1 CREB, CREB-1 cAMP responsive element binding protein 1 cyclic AMP-responsive element-binding protein 1|active transcription factor CREB|cAMP-response element-binding protein-1|cyclic adenosine 3,5-monophosphate response element binding protein|cyclic adenosine 3,5-monophosphate response element-binding protein CREB|transactivator protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CREB1, check out the CREB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CREB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CREB (Phospho-S133) CREB1 Antibody (A00577S133-1)
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