Click image to see more details
Product Info Summary
| SKU: | A12898 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CPAMD8 Antibody Picoband®
SKU/Catalog Number
A12898
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CPAMD8 Antibody Picoband® catalog # A12898. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CPAMD8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12898)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CPAMD8 recombinant protein (Position: R58-D234).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A12898 is reactive to CPAMD8 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
207 kDa
Calculated molecular weight
206.702kDa
Background of CPAMD8
CPAMD8 belongs to the complement component-3 (C3; 120700)/alpha-2-macroglobulin (A2M; 103950) family of proteins, which are involved in innate immunity and damage control. The encoded protein is membrane-associated and proteolytically processed to generate two chains. Mutations in this gene cause a form of anterior segment dysgenesis, a developmental disorder of the eye. By genomic sequence analysis, the CPAMD8 gene is mapped to chromosome 19p13.3-p13.2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12898 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissue, human T-47D whole cell, human U2OS whole cell, human K562 whole cell, human THP-1 whole cell, monkey COS-7 whole cell
IHC: human lung cancer tissue, human mammary cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CPAMD8 using anti-CPAMD8 antibody (A12898).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human THP-1 whole cell lysates,
Lane 6: monkey COS-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPAMD8 antigen affinity purified polyclonal antibody (Catalog # A12898) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPAMD8 at approximately 207KD. The expected band size for CPAMD8 is at 207KD.
Click image to see more details
Figure 2. IHC analysis of CPAMD8 using anti-CPAMD8 antibody (A12898).
CPAMD8 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CPAMD8 Antibody (A12898) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CPAMD8 using anti-CPAMD8 antibody (A12898).
CPAMD8 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CPAMD8 Antibody (A12898) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CPAMD8 (Source: Uniprot.org, NCBI)
Gene Name
CPAMD8
Full Name
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8
Weight
206.702kDa
Superfamily
protease inhibitor I39 (alpha-2-macroglobulin) family
Alternative Names
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8; CPAMD8; KIAA1283 CPAMD8 ASGD8, K-CAP, VIP C3 and PZP like alpha-2-macroglobulin domain containing 8 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8|alpha-2 macroglobulin family protein VIP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CPAMD8, check out the CPAMD8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CPAMD8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CPAMD8 Antibody Picoband® (A12898)
Hello CJ!
No publications found for A12898
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CPAMD8 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CPAMD8 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-CPAMD8 Antibody Picoband®
Question
We are currently using anti-CPAMD8 antibody A12898 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
K. Bhatt
Verified customer
Asked: 2013-06-03
Answer
The anti-CPAMD8 antibody (A12898) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-03
