Product Info Summary
SKU: | A02909 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Collagen XI alpha COL11A1 Antibody
SKU/Catalog Number
A02909
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Collagen XI alpha COL11A1 Antibody (Catalog # A02909). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen XI alpha COL11A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02909)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human Collagen XI alpha1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02909 is reactive to COL11A1 in Human, Mouse
Reconstitution
Calculated molecular weight
181065 MW
Background of COL11A1
May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
Yoshioka H., J. Biol. Chem. 265:6423-6426(1990).
Annunen S., Am. J. Hum. Genet. 65:974-983(1999).
Zhidkova N.I., J. Biol. Chem. 270:9486-9493(1995).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02909 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from K562 cells, using Collagen XI alph1 antibody A02909.
Protein Target Info & Infographic
Gene/Protein Information For COL11A1 (Source: Uniprot.org, NCBI)
Gene Name
COL11A1
Full Name
Collagen alpha-1(XI) chain
Weight
181065 MW
Superfamily
fibrillar collagen family
Alternative Names
Collagen alpha-1 (XI) chain;COL11A1;COLL6; COL11A1 CO11A1, COLL6, DFNA37, STL2 collagen type XI alpha 1 chain collagen alpha-1(XI) chain|collagen XI, alpha-1 polypeptide|collagen, type XI, alpha 1|deafness, autosomal dominant 37
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL11A1, check out the COL11A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL11A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen XI alpha COL11A1 Antibody (A02909)
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No publications found for A02909
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Customer Q&As
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5 Customer Q&As for Anti-Collagen XI alpha COL11A1 Antibody
Question
Would A02909 anti-Collagen XI alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
You can see on the product datasheet, A02909 anti-Collagen XI alpha antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-02
Question
We are currently using anti-Collagen XI alpha antibody A02909 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The anti-Collagen XI alpha antibody (A02909) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-27
Question
Is a blocking peptide available for product anti-Collagen XI alpha antibody (A02909)?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We do provide the blocking peptide for product anti-Collagen XI alpha antibody (A02909). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-17
Question
Would anti-Collagen XI alpha antibody A02909 work for WB with blood?
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
According to the expression profile of blood, COL11A1 is highly expressed in blood. So, it is likely that anti-Collagen XI alpha antibody A02909 will work for WB with blood.
Boster Scientific Support
Answered: 2018-01-24
Question
Will anti-Collagen XI alpha antibody A02909 work on dog WB with tibia?
S. Evans
Verified customer
Asked: 2018-01-08
Answer
Our lab technicians have not tested anti-Collagen XI alpha antibody A02909 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Collagen XI alpha antibody A02909 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog tibia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-08