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Key=entity_id, value=6125
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=A01411
Key=has_options, value=1
Key=required_options, value=1
Key=created_at, value=2016-10-13 08:46:37
Key=updated_at, value=2024-10-30 05:01:29
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Anti-Collagen Type IV COL4A1 Antibody
Key=cj_search_index
Key=publication_count, value=2
Key=qty_in_warehouses
Key=is_recurring, value=No
Key=visibility, value=Catalog, Search
Key=tax_class_id, value=Taxable Goods
Key=featured, value=No
Key=featured_image, value=Yes
Key=fb_product, value=
Key=custom_product_page, value=No
Key=free_shipping, value=No
Key=template, value=antibodies
Key=host, value=Rabbit
Key=quantity_and_stock_status
Key=googleshopping_exclude, value=No
Key=yoast_keyword_score, value=-1199
Key=yoast_content_score, value=30
Key=price, value=646.0000
Key=weight, value=1.0000
Key=meta_title, value=Anti-Collagen Type IV COL4A1 Antibody
Key=meta_description, value=Boster Bio Anti-Collagen Type IV COL4A1 Antibody (Catalog # A01411). Tested in Dot blot, IHC applications. This antibody reacts with Human, Bovine. Supplied as 100ug in Liquid form antibody.
Key=image, value=/antibody/A01411-01-collagen-type-iv-antibody-1-ihc-4x3__.jpg
Key=small_image, value=/antibody/A01411-01-collagen-type-iv-antibody-1-ihc-4x3__.jpg
Key=thumbnail, value=/antibody/A01411-01-collagen-type-iv-antibody-1-ihc-4x3__.jpg
Key=url_key, value=anti-collagen-type-iv-antibody-a01411-boster
Key=url_path, value=anti-collagen-type-iv-antibody-a01411-boster
Key=options_container, value=Product Info Column
Key=image_label, value=Collagen IV was detected in paraffin-embedded sections of human kidney tissue (Left) and human liver tissue (Right) using rabbit anti-Collagen Type IV polyclonal antibody (Catalog # A01411). Images provided courtesy of LifeSpan Biosciences, Seattle
Key=small_image_label, value=Collagen IV was detected in paraffin-embedded sections of human kidney tissue (Left) and human liver tissue (Right) using rabbit anti-Collagen Type IV polyclonal antibody (Catalog # A01411). Images provided courtesy of LifeSpan Biosciences, Seattle
Key=thumbnail_label, value=Collagen IV was detected in paraffin-embedded sections of human kidney tissue (Left) and human liver tissue (Right) using rabbit anti-Collagen Type IV polyclonal antibody (Catalog # A01411). Images provided courtesy of LifeSpan Biosciences, Seattle
Key=msrp_display_actual_price_type, value=
Key=gift_message_available, value=No
Key=size, value=100ug
Key=gene_full_name, value=Collagen alpha-1(IV) chain
Key=uniprot_id, value=P02462
Key=applications, value=Dot blot, IHC
Key=reactivity, value=Bovine, Human
Key=form, value=Liquid (sterile filtered)
Key=concentration, value=1.17 mg/mL by UV absorbance at 280 nm
Key=cross_reactivity, value=No cross reactivity with other proteins.
Key=predicted_reactivity, value=Bovine, Mammalian
Key=source_company, value=Rockland 600-401-106-0.1; Cost: 358.4
Key=isotype, value=IgG
Key=molecular_weight, value=160615 MW
Key=sample_size_available, value=25ug for $99, contact us for details
Key=related_product_skus, value=M01411,PA1536,PA1536-1,PB9099
Key=product_category, value=Primary Antibodies
Key=research_category, value=Extracellular Matrix, Invasion/Microenvironment, Mesenchymal Stem Cells, Signal Transduction, Stem Cells
Key=clonality, value=Polyclonal
Key=swatch_image, value=/antibody/A00003_1-WB-anti-mtor-tor-antibody.jpg
Key=rating_value, value=100
Key=review_count, value=5
Key=phospho_site, value=Phosphorylated, Thr449
Key=mp_exclude_sitemap, value=No
Key=yoast_facebook_image, value=/a/0/a01263-hrp-beta-actin-primary-antibodies-wb-testing-1.jpg
Key=yoast_twitter_image, value=/a/0/a01263-hrp-beta-actin-primary-antibodies-wb-testing-1.jpg
Key=cj_related_products, value=Acquired Porencephaly:MA1030|PB9099|M01411^Hemiplegia:A02278|MA1030|PB9099
Key=description, value=Boster Bio Anti-Collagen Type IV COL4A1 Antibody (Catalog # A01411). Tested in Dot blot, IHC applications. This antibody reacts with Human, Bovine.
Key=short_description, value=Boster Bio Anti-Collagen Type IV COL4A1 Antibody (Catalog # A01411). Tested in Dot blot, IHC applications. This antibody reacts with Human, Bovine.
Key=meta_keyword, value=Anti-Collagen Type IV COL4A1 Antibody
Key=contents, value=0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Key=background, value=Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Boster extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
Key=gene_name, value=COL4A1-COL4A6
Key=synonyms, value=Collagen Type IV, Arresten, Canstatin, Collagen Of Basement Membrane Alpha 1 Chain, Collagen alpha-1 (IV) chain, COL4A1
Key=application_details, value=ELISA: 1:5,000 - 1:50,000
IHC: 1:50 - 1:200
IF Microscopy: User optimized
IP: 1:100
WB: 1:1,000 - 1:10,000
Key=subcellular_localization, value= Secreted, extracellular space, extracellular matrix, basement membrane.
Key=tissue_specificity, value= Highly expressed in placenta.
Key=protein_function, value= Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Key=application_notes, value=Anti-Collagen Type IV has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.
Key=competitor_equivalent_skus, value=sc 9302|sc 9301|sc 385017
Key=immunogen, value=Collagen Type IV from human and bovine placenta
Key=purification, value=Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.  This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens.  Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Key=storage, value=Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
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