Product Info Summary
SKU: | A03433-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-TSG6/TNFAIP6 Antibody Picoband™
SKU/Catalog Number
A03433-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TSG6/TNFAIP6 Antibody Picoband™ catalog # A03433-1. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TSG6/TNFAIP6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03433-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TSG6, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03433-1 is reactive to TNFAIP6 in Human, Rat
Applications
A03433-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
31 kDa.
Calculated molecular weight
31203 MW
Background of TSG-6
Tumor necrosis factor-inducible gene 6 protein, also known as TSG-6, is a protein that in humans is encoded by the TNFAIP6 gene. The protein encoded by this gene is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhance the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. This gene can be induced by proinflammatory cytokines such as tumor necrosis factor alpha and interleukin-1. Enhanced levels of this protein are found in the synovial fluid of patients with osteoarthritis and rheumatoid arthritis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Validation Images & Assay Conditions
![a03433 1 1 WB anti tsg6 antibody a03433 1 1 WB anti tsg6 antibody](https://www.bosterbio.com/media/catalog/product/antibody/a03433-1-1-WB-anti-tsg6-antibody.jpg)
Click image to see more details
Figure 1. Western blot analysis of TSG6 using anti-TSG6 antibody (A03433-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG6 antigen affinity purified polyclonal antibody (Catalog # A03433-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSG6 at approximately 31 kDa. A specific band was detected for TSG6 at approximately 31 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TNFAIP6 (Source: Uniprot.org, NCBI)
Gene Name
TNFAIP6
Full Name
Tumor necrosis factor-inducible gene 6 protein
Weight
31203 MW
Alternative Names
Hyaluronate-binding protein; TNF alpha-induced protein 6; TNFAIP6; TNF-stimulated gene 6 protein; TSG6; TSG-6; TSG-6tumor necrosis factor alpha-inducible protein 6; TSG6tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor alpha-induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-stimulated gene-6 protein TNFAIP6 TSG-6, TSG6 TNF alpha induced protein 6 tumor necrosis factor-inducible gene 6 protein|TNF-stimulated gene 6 protein|hyaluronate-binding protein|tumor necrosis factor alpha-inducible protein 6|tumor necrosis factor, alpha induced protein 6|tumor necrosis factor-stimulated gene-6 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFAIP6, check out the TNFAIP6 Infographic
![TNFAIP6 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFAIP6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TSG6/TNFAIP6 Antibody Picoband™ (A03433-1)
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Customer Q&As
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1 Customer Q&As for Anti-TSG6/TNFAIP6 Antibody Picoband™
Question
We are currently using anti-TSG6/TNFAIP6 antibody A03433-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The anti-TSG6/TNFAIP6 antibody (A03433-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-18