Anti-Collagen II/COL2A1 Antibody Picoband®

Collagen II antibody

Boster Bio Anti-Collagen II/COL2A1 Antibody Picoband® catalog # A00517-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 24 publication(s).

Product Info Summary

SKU: A00517-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Collagen II/COL2A1 Antibody Picoband®

View all Collagen II Antibodies

SKU/Catalog Number

A00517-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Collagen II/COL2A1 Antibody Picoband® catalog # A00517-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Collagen II/COL2A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00517-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II, which shares 91.7% and 95.8% amino acid (aa) sequence identity with mouse and rat Collagen II, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00517-1 is reactive to COL2A1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

142 kDa

Calculated molecular weight

141.785kDa

Background of Collagen II

Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00517-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Positive Control

WB: rat cartilage tissue, mouse cartilage tissue, mouse auricular cartilage

Validation Images & Assay Conditions

Gene/Protein Information For COL2A1 (Source: Uniprot.org, NCBI)

Gene Name

COL2A1

Full Name

Collagen alpha-1(II) chain

Weight

141.785kDa

Superfamily

fibrillar collagen family

Alternative Names

Collagen alpha-1 (II) chain; Alpha-1 type II collagen; Collagen alpha-1 (II) chain; Chondrocalcin COL2A1 ANFH, AOM, COL11A3, SEDC, STL1 collagen type II alpha 1 chain collagen alpha-1(II) chain|alpha-1 type II collagen|arthroophthalmopathy, progressive (Stickler syndrome)|cartilage collagen|chondrocalcin|collagen II, alpha-1 polypeptide|collagen, type II, alpha 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on COL2A1, check out the COL2A1 Infographic

COL2A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for COL2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00517-1 has been cited in 24 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Curcumin Supplementation Enhances Bone Marrow Mesenchymal Stem Cells to Promote the Anabolism of Articular Chondrocytes and Cartilage Repair:

GPx1 knockdown suppresses chondrogenic differentiation of ATDC5 cells through induction of reductive stress

Fluoride regulates chondrocyte proliferation and autophagy via PI3K/AKT/mTOR signaling pathway

An injectable collagen-genipin-carbon dot hydrogel combined with photodynamic therapy to enhance chondrogenesis
Species: Mouse

PPARγ preservation via promoter demethylation alleviates osteoarthritis in mice
Species: Human

Zhang R,Zhang Q,Zou Z,Li Z,Jin M,An J,Li H,Ma J.Curcumin Supplementation Enhances Bone Marrow Mesenchymal Stem Cells to Promote the Anabolism of Articular Chondrocytes and Cartilage Repair.Cell Transplant.2021 Jan-Dec;30:963689721993776.doi:10.1177/096368
Species: Rat
A00517-1 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:1:200

Effects of Angelicae Pubescentis and Loranthi Decotion on repairing knee joint cartilages in rats

Microfluidics-based optimization of neuroleukin-mediated regulation of articular chondrocyte proliferation

Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro

(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor

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4 Customer Q&As for Anti-Collagen II/COL2A1 Antibody Picoband®

Question

We have been able to see staining in human fetal sternum. Are there any suggestions? Is anti-Collagen II/COL2A1 antibody supposed to stain fetal sternum positively?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

From what I have seen in literature fetal sternum does express COL2A1. From what I have seen in Uniprot.org, COL2A1 is expressed in tibia, blood, embryonic stem cell muscle, fetal sternum, among other tissues. Regarding which tissues have COL2A1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8948452
Embryonic stem cell, and Muscle, Pubmed ID: 15489334
Fetal sternum, Pubmed ID: 1999183

Boster Scientific Support

Answered: 2019-09-23

Question

I am interested in using your anti-Collagen II/COL2A1 antibody for skeletal system development studies. Has this antibody been tested with western blotting on rat liver tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

We appreciate your inquiry. This A00517-1 anti-Collagen II/COL2A1 antibody is validated on rat liver tissue, mouse liver tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-13

Question

We are currently using anti-Collagen II/COL2A1 antibody A00517-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2017-07-21

Answer

The anti-Collagen II/COL2A1 antibody (A00517-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-21

Question

We were well pleased with the WB result of your anti-Collagen II/COL2A1 antibody. However we have been able to see positive staining in embryonic stem cell muscle extracellular using this antibody. Is that expected? Could you tell me where is COL2A1 supposed to be expressed?

H. Yang

Verified customer

Asked: 2014-10-15

Answer

Based on literature, embryonic stem cell muscle does express COL2A1. Generally COL2A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL2A1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8948452
Embryonic stem cell, and Muscle, Pubmed ID: 15489334
Fetal sternum, Pubmed ID: 1999183

Boster Scientific Support

Answered: 2014-10-15

Order DetailsPrice
A00517-1

100μg

$370
A00517-1-10ug

10μg sample (liquid)

$99
A00517-1-Biotin

100 μg Biotin conjugated

$570
A00517-1-Cy3

100 μg Cy3 conjugated

$570
A00517-1-Dylight488

100 μg Dylight488 conjugated

$570
A00517-1-Dylight550

100 μg Dylight550 conjugated

$570
A00517-1-Dylight594

100 μg Dylight594 conjugated

$570
A00517-1-FITC

100 μg FITC conjugated

$570
A00517-1-HRP

100 μg HRP conjugated

$570
A00517-1-APC

100 μg APC conjugated

$670
A00517-1-PE

100 μg PE conjugated

$670
A00517-1-iFluor647

100 μg iFluor647 conjugated

$670
A00517-1-carrier-free

Carrier Free

$370

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A00517-1
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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