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Product Info Summary
| SKU: | A04554 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-COL9A1 Antibody
SKU/Catalog Number
A04554
Size
100μl
Form
Liquid
Description
Boster Bio Anti-COL9A1 Antibody catalog # A04554. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-COL9A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04554)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from COL9A1 . at AA range: 390-470
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04554 is reactive to COL9A1 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
91869 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04554 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of HepG2 cells using COL9A1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For COL9A1 (Source: Uniprot.org, NCBI)
Gene Name
COL9A1
Full Name
Collagen alpha-1(IX) chain
Weight
91869 MW
Superfamily
fibril-associated collagens with interrupted helices (FACIT) family
Alternative Names
Collagen alpha-1 (IX) chain;COL9A1; COL9A1 DJ149L1.1.2, EDM6, MED, STL4 collagen type IX alpha 1 chain collagen alpha-1(IX) chain|alpha-1(IX) collagen chain|cartilage-specific short collagen|collagen IX, alpha-1 polypeptide|collagen, type IX, alpha 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL9A1, check out the COL9A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL9A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-COL9A1 Antibody (A04554)
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No publications found for A04554
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-COL9A1 Antibody
Question
Can you help my question with product A04554, anti-COL9A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04554 anti-COL9A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-24
Question
Our lab want to know about to test anti-COL9A1 antibody A04554 on mouse tibia for research purposes, then I may be interested in using anti-COL9A1 antibody A04554 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
The products we sell, including anti-COL9A1 antibody A04554, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-24
Question
See attached the WB image, lot number and protocol we used for tibia using anti-COL9A1 antibody A04554. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-22
Question
I was wanting to use your anti-COL9A1 antibody for WB for mouse tibia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse tibia identification?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
It shows on the product datasheet, A04554 anti-COL9A1 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse tibia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-18
Question
Is there a BSA free version of anti-COL9A1 antibody A04554 available?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-COL9A1 antibody A04554 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-11
Question
Would anti-COL9A1 antibody A04554 work on goat WB with brain mammary gland?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Our lab technicians have not validated anti-COL9A1 antibody A04554 on goat. You can run a BLAST between goat and the immunogen sequence of anti-COL9A1 antibody A04554 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat brain mammary gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-16
Question
Would anti-COL9A1 antibody A04554 work for WB with tibia?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
According to the expression profile of tibia, COL9A1 is highly expressed in tibia. So, it is likely that anti-COL9A1 antibody A04554 will work for WB with tibia.
Boster Scientific Support
Answered: 2019-11-20
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tibia using anti-COL9A1 antibody A04554. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
I see that the anti-COL9A1 antibody A04554 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-14
Question
Is a blocking peptide available for product anti-COL9A1 antibody (A04554)?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
We do provide the blocking peptide for product anti-COL9A1 antibody (A04554). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-27
Question
We are currently using anti-COL9A1 antibody A04554 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?
G. Krishna
Verified customer
Asked: 2019-02-08
Answer
The anti-COL9A1 antibody (A04554) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-08
Question
Does A04554 anti-COL9A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Walker
Verified customer
Asked: 2015-12-28
Answer
As indicated on the product datasheet, A04554 anti-COL9A1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-12-28
Question
Is this A04554 anti-COL9A1 antibody reactive to the isotypes of COL9A1?
G. Jones
Verified customer
Asked: 2015-11-23
Answer
The immunogen of A04554 anti-COL9A1 antibody is Synthesized peptide derived from the Internal region of human COL9A1. at AA rangle: 390-470. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-11-23
