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Product Info Summary
| SKU: | M01683-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD146 MCAM Rabbit Monoclonal Antibody
View all MCAM/CD146 Antibodies
SKU/Catalog Number
M01683-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD146 MCAM Rabbit Monoclonal Antibody catalog # M01683-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD146 MCAM Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01683-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AbC83
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD146
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01683-1 is reactive to MCAM in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120 kDa
Calculated molecular weight
71607 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01683-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human U2OS whole cell, human K562 whole cell, human Hela whole cell, human A549 whole cell, rat lung tissue, rat spleen tissue, mouse lung tissue
IHC: human placenta tissue
ICC/IF: A375 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD146 using anti-CD146 antibody (M01683-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD146 antigen affinity purified monoclonal antibody (Catalog # M01683-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD146 at approximately 120 kDa. The expected band size for CD146 is at 72 kDa.
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Figure 2. IHC analysis of CD146 using anti-CD146 antibody (M01683-1).
CD146 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD146 Antibody (M01683-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Immunofluorescent analysis of A375 cells, using CD146 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MCAM (Source: Uniprot.org, NCBI)
Gene Name
MCAM
Full Name
Cell surface glycoprotein MUC18
Weight
71607 MW
Alternative Names
Cell surface glycoprotein MUC18;Cell surface glycoprotein P1H12;Melanoma cell adhesion molecule;Melanoma-associated antigen A32;Melanoma-associated antigen MUC18;S-endo 1 endothelial-associated antigen;CD146;MCAM;MUC18; MCAM CD146, HEMCAM, METCAM, MUC18, MelCAM melanoma cell adhesion molecule cell surface glycoprotein MUC18|Gicerin|S-endo 1 endothelial-associated |cell surface glycoprotein P1H12|melanoma adhesion molecule|melanoma-associated A32|melanoma-associated MUC18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCAM, check out the MCAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD146 MCAM Rabbit Monoclonal Antibody (M01683-1)
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Customer Q&As
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5 Customer Q&As for Anti-CD146 MCAM Rabbit Monoclonal Antibody
Question
For Anti-CD146 Rabbit Monoclonal Antibody M01683-1 (Abways CY5209), could you ask them for the immunogen sequence?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The immunogen sequence of M01683-1 (Abways CY5209) is aa628-640: SGDKRAPGDQGEK.
Boster Scientific Support
Answered: 2020-03-31
Question
We are currently using anti-CD146 Rabbit Monoclonal antibody M01683-1 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The anti-CD146 Rabbit Monoclonal antibody (M01683-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-03
Question
We have been able to see staining in human popliteal artery. Are there any suggestions? Is anti-CD146 Rabbit Monoclonal antibody supposed to stain popliteal artery positively?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
According to literature popliteal artery does express MCAM. According to Uniprot.org, MCAM is expressed in popliteal artery, melanoma, trachea, aortic endothelium, pns, cervix carcinoma, liver, among other tissues. Regarding which tissues have MCAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Liver, Pubmed ID: 19159218
Melanoma, Pubmed ID: 2602381, 8378324
PNS, Pubmed ID: 15489334
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-05-22
Question
We ordered your anti-CD146 Rabbit Monoclonal antibody for IF on aortic endothelium in the past. I am using human, and I plan to use the antibody for IHC next. I am looking for examining aortic endothelium as well as pns in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-05-19
Answer
I looked at the website and datasheets of our anti-CD146 Rabbit Monoclonal antibody and it seems that M01683-1 has been validated on human in both IF and IHC. Thus M01683-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-05-19
Question
Our team were well pleased with the WB result of your anti-CD146 Rabbit Monoclonal antibody. However we have observed positive staining in popliteal artery membrane using this antibody. Is that expected? Could you tell me where is MCAM supposed to be expressed?
J. Miller
Verified customer
Asked: 2015-04-14
Answer
According to literature, popliteal artery does express MCAM. Generally MCAM expresses in membrane. Regarding which tissues have MCAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Liver, Pubmed ID: 19159218
Melanoma, Pubmed ID: 2602381, 8378324
PNS, Pubmed ID: 15489334
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-04-14
