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Product Info Summary
| SKU: | PA1428-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Cyclin-dependent kinase 4 Cdk4 Antibody Picoband®
SKU/Catalog Number
PA1428-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cyclin-dependent kinase 4 Cdk4 Antibody catalog # PA1428-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin-dependent kinase 4 Cdk4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1428-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cdk4, different from the related rat sequence by one amino acid, and from the related human sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1428-1 is reactive to Cdk4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
34 kDa
Calculated molecular weight
33751 MW
Background of CDK4
Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1428-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human K562 whole cell, rat PC-12 whole cell, mosue NIH/3T3 whole cell, mosue RAW2647 whole cell
ICC/IF: MCF-7 cell
FCM: MCF-7 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CDK4 using anti-CDK4 antibody (PA1428-1, Left) and anti-CDK4 antibody (PB9535, Right).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mosue NIH/3T3 whole cell lysates,
Lane 5: mosue RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 antigen affinity purified polyclonal antibody (Catalog # PA1428-1) and rabbit anti-CDK4 antigen affinity purified polyclonal antibody (Catalog # PB9535)at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK4 at approximately 34 kDa. The expected band size for CDK4 is at 34 kDa.
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Figure 2. IF analysis of CDK4 using anti-CDK4 antibody (PA1428-1) and anti-Tubulin Alpha antibody (M03989-3).
CDK4 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CDK4 Antibody (PA1428-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-CDK4 antibody (PA1428-1).
Overlay histogram showing MCF-7 cells stained with PA1428-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDK4 Antibody (PA1428-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Cdk4 (Source: Uniprot.org, NCBI)
Gene Name
Cdk4
Full Name
Cyclin-dependent kinase 4
Weight
33751 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-dependent kinase 4;2.7.11.22;CRK3;Cell division protein kinase 4;PSK-J3;Cdk4;Crk3; CDK4 CMM3, PSK-J3 cyclin dependent kinase 4 cyclin-dependent kinase 4|cell division protein kinase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cdk4, check out the Cdk4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cdk4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin-dependent kinase 4 Cdk4 Antibody Picoband® (PA1428-1)
Hello CJ!
PA1428-1 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
Effect of Bone Morphogenetic Protein-2 on Proliferation and Apoptosis of Gastric Cancer Cells
Liu W, Dai Q, Lu N, Wei L, Ha J, Rong J, Mu R, You Q, Li Z, Guo Q. Biochem Cell Biol. 2011 Jun;89(3):287-98. Doi: 10.1139/O10-162. Epub 2011 Apr 14. Lyg-202 Inhibits The Proliferation Of Human Colorectal Carcinoma Hct-116 Cells Through Induction O...
Wang Hy, Huang Rp, Han P, Xue Db, Li Hb, Liu B, Shan P, Wang Qs, Li Ks, Li Hl. Cell Biochem Funct. 2014 Mar;32(2):201-8. Doi: 10.1002/Cbf.2995. Epub 2013 Sep 17. The Effects Of Artemisinin On The Proliferation And Apoptosis Of Vascular Smooth Musc...
Li Bh, Xu Sb, Li F, Zou Xg, Saimaiti A, Simayi D, Wang Yh, Zhang Y, Yuan J, Zhang Wj. Cell Signal. 2012 Mar;24(3):718-25. Doi: 10.1016/J.Cellsig.2011.11.005. Epub 2011 Nov 13. Stat6 Activity-Related Th2 Cytokine Profile And Tumor Growth Advantage ...
Zhang H, Zhao B, Huang C, Meng Xm, Bian Eb, Li J. Plos One. 2014 May 2;9(5):E95520. Doi: 10.1371/Journal.Pone.0095520. Ecollection 2014. Melittin Restores Pten Expression By Down-Regulating Hdac2 In Human Hepatocelluar Carcinoma Hepg2 Cells.
Han W, Liu Gn. Life Sci. 2010 Feb 13;86(7-8):234-43. Doi: 10.1016/J.Lfs.2009.12.005. Epub 2009 Dec 16. Egr-1 Decoy Odns Inhibit Vascular Smooth Muscle Cell Proliferation And Neointimal Hyperplasia Of Balloon-Injured Arteries In Rat.
Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.
Zhou R, Lu Z, Liu K, Guo J, Liu J, Zhou Y, Yang J, Mi M, Xu H. Curr Cancer Drug Targets. 2015;14(9):860-71. Platycodin D Induces Tumor Growth Arrest By Activating Foxo3A Expression In Prostate Cancer In Vitro And In Vivo.
Cai K, Xu J, Zhang Y. Neurosci Lett. 2012 Jul 26;522(1):62-6. Doi: 10.1016/J.Neulet.2012.06.017. Epub 2012 Jun 15. Bone Marrow Stromal Cells Induce Cell Cycle Arrest In Reactive Astrocytes In Vitro.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Cyclin-dependent kinase 4 Cdk4 Antibody Picoband®
Question
I was wanting to use your anti-Cdk4 antibody for WB for mouse embryo on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse embryo identification?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
You can see on the product datasheet, PA1428-1 anti-Cdk4 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryo in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-06
Question
I see that the anti-Cdk4 antibody PA1428-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-23
Question
Our lab want to know about to test anti-Cdk4 antibody PA1428-1 on mouse embryo for research purposes, then I may be interested in using anti-Cdk4 antibody PA1428-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
The products we sell, including anti-Cdk4 antibody PA1428-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-13
Question
Will anti-Cdk4 antibody PA1428-1 work on feline WB with embryo?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
Our lab technicians have not validated anti-Cdk4 antibody PA1428-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Cdk4 antibody PA1428-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline embryo in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-04
Question
Is a blocking peptide available for product anti-Cdk4 antibody (PA1428-1)?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
We do provide the blocking peptide for product anti-Cdk4 antibody (PA1428-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-31
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryo using anti-Cdk4 antibody PA1428-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-22
Question
We are interested in using your anti-Cdk4 antibody for r-mmu-75815; ubiquitin-dependent degradation of cyclin d studies. Has this antibody been tested with western blotting on colo320 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
Thank you for your inquiry. This PA1428-1 anti-Cdk4 antibody is validated on rat thymus tissue, tissue lysate, hela cell lysate, a549 cell lysate, colo320 cell lysate, jurkat cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-31
Question
Is this PA1428-1 anti-Cdk4 antibody reactive to the isotypes of CDK4?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
The immunogen of PA1428-1 anti-Cdk4 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cdk4(283-303aa RISAFRALQHSYLHKEESDAE), different from the related rat sequence by one amino acid, and from the related human sequence by four amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-24
Question
I have attached the WB image, lot number and protocol we used for embryo using anti-Cdk4 antibody PA1428-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-22
Question
Would PA1428-1 anti-Cdk4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
It shows on the product datasheet, PA1428-1 anti-Cdk4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-26
Question
We are currently using anti-Cdk4 antibody PA1428-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
D. Taylor
Verified customer
Asked: 2018-03-13
Answer
The anti-Cdk4 antibody (PA1428-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-13
Question
Does anti-Cdk4 antibody PA1428-1 work for WB with embryo?
S. Gonzalez
Verified customer
Asked: 2018-02-13
Answer
According to the expression profile of embryo, CDK4 is highly expressed in embryo. So, it is likely that anti-Cdk4 antibody PA1428-1 will work for WB with embryo.
Boster Scientific Support
Answered: 2018-02-13
Question
We have observed staining in human right ovary. Are there any suggestions? Is anti-Cdk4 antibody supposed to stain right ovary positively?
D. Anderson
Verified customer
Asked: 2018-01-08
Answer
According to literature right ovary does express CDK4. According to Uniprot.org, CDK4 is expressed in right ovary, embryo, muscle, among other tissues. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-01-08
Question
Do you have a BSA free version of anti-Cdk4 antibody PA1428-1 available?
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cdk4 antibody PA1428-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-07
Question
We were well pleased with the WB result of your anti-Cdk4 antibody. However we have observed positive staining in embryo cytoplasm. using this antibody. Is that expected? Could you tell me where is CDK4 supposed to be expressed?
M. Li
Verified customer
Asked: 2015-03-06
Answer
From literature, embryo does express CDK4. Generally CDK4 expresses in cytoplasm. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-03-06
Question
I have a question about product PA1428-1, anti-Cdk4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Jones
Verified customer
Asked: 2013-08-16
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1428-1 anti-Cdk4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-08-16
