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Product Info Summary
| SKU: | RP1050 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD146/MCAM Antibody Picoband®
View all MCAM/CD146 Antibodies
SKU/Catalog Number
RP1050
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD146/MCAM Antibody catalog # RP1050. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD146/MCAM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1050)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1050 is reactive to MCAM in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
71607 MW
Background of MCAM/CD146
CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1050 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human U2OS whole cell
IHC: Human Lung Cancer Tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD146 using anti-CD146 antibody (RP1050).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD146 antigen affinity purified polyclonal antibody (Catalog # RP1050) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD146 at approximately 120 kDa. The expected band size for CD146 is at 72 kDa.
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Anti-CD146 antibody, RP1050, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For MCAM (Source: Uniprot.org, NCBI)
Gene Name
MCAM
Full Name
Cell surface glycoprotein MUC18
Weight
71607 MW
Alternative Names
Cell surface glycoprotein MUC18;Cell surface glycoprotein P1H12;Melanoma cell adhesion molecule;Melanoma-associated antigen A32;Melanoma-associated antigen MUC18;S-endo 1 endothelial-associated antigen;CD146;MCAM;MUC18; MCAM CD146, HEMCAM, METCAM, MUC18, MelCAM melanoma cell adhesion molecule cell surface glycoprotein MUC18|Gicerin|S-endo 1 endothelial-associated |cell surface glycoprotein P1H12|melanoma adhesion molecule|melanoma-associated A32|melanoma-associated MUC18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCAM, check out the MCAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD146/MCAM Antibody Picoband® (RP1050)
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No publications found for RP1050
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD146/MCAM Antibody Picoband®
Question
We have seen staining in human trachea. Are there any suggestions? Is anti-CD146/MCAM antibody supposed to stain trachea positively?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
From literature trachea does express MCAM. From Uniprot.org, MCAM is expressed in popliteal artery, melanoma, trachea, aortic endothelium, pns, cervix carcinoma, liver, among other tissues. Regarding which tissues have MCAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Liver, Pubmed ID: 19159218
Melanoma, Pubmed ID: 2602381, 8378324
PNS, Pubmed ID: 15489334
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-02
Question
Is there a BSA free version of anti-CD146/MCAM antibody RP1050 available?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD146/MCAM antibody RP1050 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-23
Question
Would anti-CD146/MCAM antibody RP1050 work for WB with popliteal artery?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
According to the expression profile of popliteal artery, MCAM is highly expressed in popliteal artery. So, it is likely that anti-CD146/MCAM antibody RP1050 will work for WB with popliteal artery.
Boster Scientific Support
Answered: 2019-12-23
Question
I was wanting to use your anti-CD146/MCAM antibody for WB for human popliteal artery on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human popliteal artery identification?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
You can see on the product datasheet, RP1050 anti-CD146/MCAM antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human popliteal artery in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-22
Question
I see that the anti-CD146/MCAM antibody RP1050 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for popliteal artery using anti-CD146/MCAM antibody RP1050. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-22
Question
Will RP1050 anti-CD146/MCAM antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
You can see on the product datasheet, RP1050 anti-CD146/MCAM antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-04
Question
I have a question about product RP1050, anti-CD146/MCAM antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1050 anti-CD146/MCAM antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-25
Question
Is a blocking peptide available for product anti-CD146/MCAM antibody (RP1050)?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
We do provide the blocking peptide for product anti-CD146/MCAM antibody (RP1050). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-23
Question
Is this RP1050 anti-CD146/MCAM antibody reactive to the isotypes of MCAM?
Verified Customer
Verified customer
Asked: 2019-02-07
Answer
The immunogen of RP1050 anti-CD146/MCAM antibody is E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-07
Question
I am interested in using your anti-CD146/MCAM antibody for cell adhesion studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
Thank you for your inquiry. This RP1050 anti-CD146/MCAM antibody is tested on a375 whole cell lysate, hela whole cell lysate, hepg2 whole cell lysate, lung cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-08-02
Question
I would like to test anti-CD146/MCAM antibody RP1050 on human popliteal artery for research purposes, then I may be interested in using anti-CD146/MCAM antibody RP1050 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Yang
Verified customer
Asked: 2018-01-24
Answer
The products we sell, including anti-CD146/MCAM antibody RP1050, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-24
Question
We are currently using anti-CD146/MCAM antibody RP1050 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
S. Miller
Verified customer
Asked: 2017-10-25
Answer
The anti-CD146/MCAM antibody (RP1050) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-25
Question
We were satisfied with the WB result of your anti-CD146/MCAM antibody. However we have been able to see positive staining in popliteal artery membrane using this antibody. Is that expected? Could you tell me where is MCAM supposed to be expressed?
R. Taylor
Verified customer
Asked: 2016-09-09
Answer
Based on literature, popliteal artery does express MCAM. Generally MCAM expresses in membrane. Regarding which tissues have MCAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Liver, Pubmed ID: 19159218
Melanoma, Pubmed ID: 2602381, 8378324
PNS, Pubmed ID: 15489334
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-09-09
Question
Here is the WB image, lot number and protocol we used for popliteal artery using anti-CD146/MCAM antibody RP1050. Please let me know if you require anything else.
O. Roberts
Verified customer
Asked: 2015-04-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-02
Question
We purchased anti-CD146/MCAM antibody for IHC on popliteal artery last year. I am using human, and We intend to use the antibody for WB next. We want examining popliteal artery as well as aortic endothelium in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
V. Li
Verified customer
Asked: 2013-02-19
Answer
I have checked the website and datasheets of our anti-CD146/MCAM antibody and it seems that RP1050 has been tested on human in both IHC and WB. Thus RP1050 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-02-19
