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Product Info Summary
| SKU: | M00159-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CDK4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00159-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDK4 Rabbit Monoclonal Antibody catalog # M00159-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00159-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GIC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDK4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00159-1 is reactive to CDK4 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
34 kDa
Calculated molecular weight
33730 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00159-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
IHC: human stomach cancer tissue
ICC/IF: MCF7 cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human stomach cancer, using CDK4 Antibody.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of MCF7 cells, using CDK4 Antibody.
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Western blot analysis of CDK4 expression in Hela cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CDK4 (Source: Uniprot.org, NCBI)
Gene Name
CDK4
Full Name
Cyclin-dependent kinase 4
Weight
33730 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-dependent kinase 4;2.7.11.22;Cell division protein kinase 4;PSK-J3;CDK4; CDK4 CMM3, PSK-J3 cyclin dependent kinase 4 cyclin-dependent kinase 4|cell division protein kinase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK4, check out the CDK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDK4 Rabbit Monoclonal Antibody (M00159-1)
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Customer Q&As
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16 Customer Q&As for Anti-CDK4 Rabbit Monoclonal Antibody
Question
Is this M00159-1 anti-CDK4 Rabbit Monoclonal antibody reactive to the isotypes of CDK4?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The immunogen of M00159-1 anti-CDK4 Rabbit Monoclonal antibody is A synthesized peptide derived from human CDK4 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-30
Question
I see that the anti-CDK4 Rabbit Monoclonal antibody M00159-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-14
Question
We are currently using anti-CDK4 Rabbit Monoclonal antibody M00159-1 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
The anti-CDK4 Rabbit Monoclonal antibody (M00159-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-19
Question
We want to test anti-CDK4 Rabbit Monoclonal antibody M00159-1 on human right ovary for research purposes, then I may be interested in using anti-CDK4 Rabbit Monoclonal antibody M00159-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The products we sell, including anti-CDK4 Rabbit Monoclonal antibody M00159-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-10
Question
Can you help my question with product M00159-1, anti-CDK4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00159-1 anti-CDK4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-27
Question
Does anti-CDK4 Rabbit Monoclonal antibody M00159-1 work for IF with right ovary?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
According to the expression profile of right ovary, CDK4 is highly expressed in right ovary. So, it is likely that anti-CDK4 Rabbit Monoclonal antibody M00159-1 will work for IF with right ovary.
Boster Scientific Support
Answered: 2020-01-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-CDK4 Rabbit Monoclonal antibody M00159-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-06
Question
Is a blocking peptide available for product anti-CDK4 Rabbit Monoclonal antibody (M00159-1)?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
We do provide the blocking peptide for product anti-CDK4 Rabbit Monoclonal antibody (M00159-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-11
Question
We bought anti-CDK4 Rabbit Monoclonal antibody for IF on muscle last year. I am using human, and We want to use the antibody for WB next. I would like examining muscle as well as embryo in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
I looked at the website and datasheets of our anti-CDK4 Rabbit Monoclonal antibody and I see that M00159-1 has been validated on human in both IF and WB. Thus M00159-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-25
Question
We have observed staining in human right ovary. Do you have any suggestions? Is anti-CDK4 Rabbit Monoclonal antibody supposed to stain right ovary positively?
A. Collins
Verified customer
Asked: 2019-06-27
Answer
According to literature right ovary does express CDK4. According to Uniprot.org, CDK4 is expressed in right ovary, embryo, muscle, among other tissues. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-27
Question
Will M00159-1 anti-CDK4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
As indicated on the product datasheet, M00159-1 anti-CDK4 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-15
Question
Is there a BSA free version of anti-CDK4 Rabbit Monoclonal antibody M00159-1 available?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CDK4 Rabbit Monoclonal antibody M00159-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-08
Question
I was wanting to use your anti-CDK4 Rabbit Monoclonal antibody for IF for human right ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human right ovary identification?
Verified Customer
Verified customer
Asked: 2018-06-21
Answer
You can see on the product datasheet, M00159-1 anti-CDK4 Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-21
Question
We are interested in using your anti-CDK4 Rabbit Monoclonal antibody for regulation of insulin receptor signaling pathway studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-10-24
Answer
Thanks for your inquiry. This M00159-1 anti-CDK4 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-10-24
Question
I have attached the WB image, lot number and protocol we used for right ovary using anti-CDK4 Rabbit Monoclonal antibody M00159-1. Please let me know if you require anything else.
J. Evans
Verified customer
Asked: 2017-05-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-17
Question
Our team were well pleased with the WB result of your anti-CDK4 Rabbit Monoclonal antibody. However we have observed positive staining in muscle cytoplasm. using this antibody. Is that expected? Could you tell me where is CDK4 supposed to be expressed?
A. Dhar
Verified customer
Asked: 2015-12-14
Answer
From literature, muscle does express CDK4. Generally CDK4 expresses in cytoplasm. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-12-14
