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Product Info Summary
| SKU: | EK1113 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Galectin-9 / Gal 9 ELISA Kit PicoKine®
View all Galectin-9 ELISA kits
SKU/Catalog Number
EK1113
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Galectin-9 / Gal 9 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Galectin-9 / Gal 9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1113)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: M1-T355
Sensitivity
<10 pg/ml
Assay Range
93.8 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1113 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1113 is reactive to LGALS9 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1113 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Galectin-9
Galectin-9, also called HUAT or LGALS9A is a protein that in humans is encoded by the LGALS9 gene. This gene is mapped to 17q11.2. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1113-CAP | Anti-Human LGALS9 Pre-coated 96-well strip microplate | 1 |
| EK1113-ST | Human LGALS9 Standard | 2 vials, 10 ng/tube |
| EK1113-DA | Human LGALS9 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.075 | 0.114 | 0.190 | 0.263 | 0.416 | 0.705 | 1.022 | 1.802 |
Data Example Images
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Human Galectin-9 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Galectin-9, Dilution ratio of 1:10, concentration in serum and plasma is around 5 ng/ml..
Some articles we found to cite concentrations of Galectin-9 in samples: 24169278 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Galectin-9 / Gal 9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 168 | 731 | 3580 | 177 | 792 | 3920 |
| Standard deviation | 7.90 | 51.9 | 218.38 | 9.55 | 66.52 | 274.4 |
| CV (%) | 4.7% | 7.1% | 6.1% | 5.4% | 8.4% | 7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Galectin-9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 168 | 165 | 163 | 179 | 168 | 6.17 | 3.6% |
| Sample 2 | 731 | 656 | 726 | 619 | 683 | 47.37 | 6.9% |
| Sample 3 | 3580 | 3263 | 3243 | 3664 | 3437 | 187 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For LGALS9 (Source: Uniprot.Org, NCBI)
Gene Name
LGALS9
Full Name
Galectin-9
Weight
39518 MW
Alternative Names
Galectin-9;Gal-9;Ecalectin;Tumor antigen HOM-HD-21;LGALS9; LGALS9 HUATA, LGALS9 galectin 9 galectin-9|ecalectin|gal-9|lectin, galactoside-binding, soluble, 9|tumor HOM-HD-21|urate transporter/channel protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LGALS9, check out the LGALS9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LGALS9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Galectin-9 / Gal 9 ELISA Kit PicoKine® (EK1113)
Hello CJ!
EK1113 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Circulating T Cells Exhibit Different TIM3/Galectin-9 Expression in Patients with Obesity and Obesity-Related Diabetes
Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Human Galectin-9 / Gal 9 ELISA Kit PicoKine®
Question
Q: Can Galectin-9 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-12-10
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-12-10
Question
The EK1113 instructions for diluting standards states to “mix thoroughly”, do you recommend vortexing the standards, or a more gentle mixing technique like pipetting or inversion?
Verified customer
Asked: 2019-05-13
Answer
For the Human Galectin-9 / Gal 9 ELISA Kit PicoKine™ (EK1113), vortexing the standards is acceptable. Bubbles should be avoided. Taking up and dispensing the standard solution repeatedly using a pipette is also acceptable.
Boster Scientific Support
Answered: 2019-05-13
Question
A plate shaker is not mentioned in the K1113 instructions. Are plates always incubated without any shaking? If plates are shaken during incubations, approximately what RPM is used?
Verified customer
Asked: 2019-05-13
Answer
For the Human Galectin-9 / Gal 9 ELISA Kit PicoKine™ (EK1113), the plate shaker is used to accelerate the reaction. Incubation at 37°C is also able to achieve the same effect. It is unnecessary to shake the plate during incubation.
Boster Scientific Support
Answered: 2019-05-13
Question
What is the concentration of sodium azide contained in each kit component? 1) lyophilized recombinant Human LGALS9 standard 2) biotinylated anti-Human LGALS9 antibody 3) Avidin-Biotin-Peroxidase Complex(ABC) 4) sample diluent buffer 5) antibody diluent buffer 6) ABC diluent buffer
Verified customer
Asked: 2019-03-26
Answer
For Human Galectin-9 / Gal 9 ELISA Kit PicoKine™ (EK1113) concentration of sodium azide: 1) 0.02mg/vial 2) 0.02% 3) 0.02% 4) 0.02% 5) 0.02% 6) 0.02%
Boster Scientific Support
Answered: 2019-03-27
Question
Does lyophilized recombinant Human LGALS 9 standard contain sodium azide?
Verified customer
Asked: 2019-03-26
Answer
lyophilized recombinant Human LGALS 9 standard contains 0.02% Sodium azide.
Boster Scientific Support
Answered: 2019-03-27
Question
What is the origin of the lyophilized recombinant Human LGALS9 standard in EK1113?
Verified customer
Asked: 2019-03-26
Answer
lyophilized recombinant Human LGALS9 standard origin in the Human Galectin-9 / Gal 9 ELISA Kit PicoKine™ (EK1113) is E.coli.
Boster Scientific Support
Answered: 2019-03-27
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
C. Anderson
Verified customer
Asked: 2019-02-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-02-12
Question
Q: What is the optimal O.D. value for Galectin-9 ELISA kit? I used your Galectin-9 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Galectin-9 even though the O.D. values are not very high?
C. Tiwari
Verified customer
Asked: 2019-02-08
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Galectin-9 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Galectin-9 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-02-08
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Galectin-9 using the EK1113 Human Galectin-9 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-12-03
Question
Q: can you suggest the dilution ratio of serum samples for detection of Galectin-9 in Human cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
K. Lee
Verified customer
Asked: 2018-11-03
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Galectin-9 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-11-03
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
A. Anderson
Verified customer
Asked: 2018-04-15
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-04-15
Question
Q: can I use citrate plasma as samples in Human Galectin-9 Picokine® ELISA Kit (Catalog # EK1113)?
Verified Customer
Verified customer
Asked: 2016-12-18
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Galectin-9 causing disruption of its protein structure. Galectin-9 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Galectin-9 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-12-18
Question
Later this week I will be working with kit EK1113 to quantitate Human LGALS9 and I have a couple questions. A plate shaker is not mentioned in the kit instructions. Are plates always incubated without any shaking? If plates are shaken during incubations, approximately what RPM is used? The instructions for diluting standards states to
Verified Customer
Verified customer
Asked: 2016-09-22
Answer
Please see the following responses to the questions. 1. Plate shaker is used to accelarate the reaction and incubation at 37
Boster Scientific Support
Answered: 2016-09-22
