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Product Info Summary
| SKU: | PA1102 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SKP2 Antibody
SKU/Catalog Number
PA1102
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SKP2 Antibody catalog # PA1102. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SKP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1102)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SKP2, different from the related rat and mouse sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1102 is reactive to SKP2 in Human, Mouse, Rat
Applications
PA1102 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
25 kDa
Calculated molecular weight
47761 MW
Background of SKP2
The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G (1)-S transition by controlling the stability of several G (1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
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Anti-SKP2 antibody, PA1102, Western blotting
WB: MCF-7 Cell Lysate
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Anti-SKP2 antibody, PA1102, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For SKP2 (Source: Uniprot.org, NCBI)
Gene Name
SKP2
Full Name
S-phase kinase-associated protein 2
Weight
47761 MW
Alternative Names
CDK2/cyclin A-associated protein p45; cyclin A/CDK2-associated protein p45; Cyclin-A/CDK2-associated protein p45; FBL1; F-box protein Skp2; F-box/LRR-repeat protein 1; FBXL1; FBXL1MGC1366; FLB1; p45skp2; Skp2; S-phase kinase-associated protein 2 (p45); S-phase kinase-associated protein 2 SKP2 FBL1, FBXL1, FLB1, p45 S-phase kinase associated protein 2 S-phase kinase-associated protein 2|CDK2/cyclin A-associated protein p45|F-box/LRR-repeat protein 1|S-phase kinase-associated protein 2, E3 ubiquitin protein ligase|p45skp2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SKP2, check out the SKP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SKP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SKP2 Antibody (PA1102)
Hello CJ!
PA1102 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Su Y, Wang F, Yan Q, Teng Y, Cui H. Mol Vis. 2010 May 25;16:907-15. Inhibition Of Proliferation Of Rabbit Lens Epithelial Cells By S-Phase Kinase-Interacting Protein 2 Targeting Small Interfering Rna.
Xu F, Zhu X, Han T, You X, Liu F, Ye L, Zhang X, Wang X, Yao Y. Int J Oncol. 2014 Jul;45(1):255-63. Doi: 10.3892/Ijo.2014.2411. Epub 2014 Apr 29. The Oncoprotein Hepatitis B X-Interacting Protein Promotes The Migration Of Ovarian Cancer Cells Thro...
Xu F, You X, Liu F, Shen X, Yao Y, Ye L, Zhang X. Cancer Lett. 2013 Jun 1;333(1):124-32. Doi: 10.1016/J.Canlet.2013.01.029. Epub 2013 Jan 22. The Oncoprotein Hbxip Up-Regulates Skp2 Via Activating Transcription Factor E2F1 To Promote Proliferation...
Su Y, Wang F, Qi H, Zhao Sg, Li X, Cui H. Mol Vis. 2011 Jan 22;17:247-56. Small Interfering Rna Targeting Of S Phase Kinase-Interacting Protein 2 Inhibits Cell Proliferation Of Pterygium Fibroblasts.
Qiu J, Zhang C, Lv Y, Zhang Y, Zhu C, Wang X, Yao W. Neurochem Int. 2013 Aug;63(2):87-92. Doi: 10.1016/J.Neuint.2013.05.009. Epub 2013 May 28. Cdh1 Inhibits Reactive Astrocyte Proliferation After Oxygen-Glucose Deprivation And Reperfusion.
Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...
Chen Xm, Bai Y, Zhong Yj, Xie Xl, Long Hw, Yang Yy, Wu Sg, Jia Q, Wang Xh. Plos One. 2013 Nov 12;8(11):E79201. Doi: 10.1371/Journal.Pone.0079201. Ecollection 2013. Wogonin Has Multiple Anti-Cancer Effects By Regulating C-Myc/Skp2/Fbw7?? And Hdac1/...
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SKP2 Antibody
Question
I see that the anti-SKP2 antibody PA1102 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-02
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for oocyte using anti-SKP2 antibody PA1102. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-30
Question
Is a blocking peptide available for product anti-SKP2 antibody (PA1102)?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
We do provide the blocking peptide for product anti-SKP2 antibody (PA1102). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-12
Question
I was wanting to use your anti-SKP2 antibody for IHC for human oocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human oocyte identification?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
You can see on the product datasheet, PA1102 anti-SKP2 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human oocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-03
Question
My question regards to test anti-SKP2 antibody PA1102 on human oocyte for research purposes, then I may be interested in using anti-SKP2 antibody PA1102 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
The products we sell, including anti-SKP2 antibody PA1102, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-15
Question
We are currently using anti-SKP2 antibody PA1102 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
G. Zhang
Verified customer
Asked: 2018-12-24
Answer
The anti-SKP2 antibody (PA1102) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-24
Question
Here is the WB image, lot number and protocol we used for oocyte using anti-SKP2 antibody PA1102. Please let me know if you require anything else.
D. Mangal
Verified customer
Asked: 2017-09-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-29
