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Product Info Summary
| SKU: | M00119 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-CDC42 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00119
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDC42 Rabbit Monoclonal Antibody catalog # M00119. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDC42 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00119)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IAE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDC42
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00119 is reactive to CDC42 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
21259 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00119 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
IP 1:30
FC 1:50
Positive Control
IHC: Rat skin tissue, Human esophageal carcinoma tissue, Human colon tissue, Human Hodgkin's lymphoma tissue, Mouse skeletal muscle - gastrocnemius tissue, Mouse skin tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Western blot analysis of CDC42 expression in (1) Jurkat cell lysate; (2) Mouse spleen lysate.
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Immunohistochemical analysis of paraffin-embedded Rat skin, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colon, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:400 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:200 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CDC42 (Source: Uniprot.org, NCBI)
Gene Name
CDC42
Full Name
Cell division control protein 42 homolog
Weight
21259 MW
Superfamily
small GTPase superfamily
Alternative Names
Cell division control protein 42 homolog;G25K GTP-binding protein;CDC42; CDC42 CDC42Hs, G25K, TKS cell division cycle 42 cell division control protein 42 homolog|G25K GTP-binding protein|GTP binding protein, 25kDa|dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD))|dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))|growth-regulating protein|small GTP binding protein CDC42
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC42, check out the CDC42 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC42: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDC42 Rabbit Monoclonal Antibody (M00119)
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No publications found for M00119
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CDC42 Rabbit Monoclonal Antibody
Question
Can you help my question with product M00119, anti-CDC42 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00119 anti-CDC42 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-17
Question
Is a blocking peptide available for product anti-CDC42 Rabbit Monoclonal antibody (M00119)?
B. Anderson
Verified customer
Asked: 2020-01-01
Answer
We do provide the blocking peptide for product anti-CDC42 Rabbit Monoclonal antibody (M00119). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-01
Question
I see that the anti-CDC42 Rabbit Monoclonal antibody M00119 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-20
Question
you antibody using your anti-CDC42 Rabbit Monoclonal antibody for nuclear migration studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
Thank you for your inquiry. This M00119 anti-CDC42 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IP, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-01
Question
We are currently using anti-CDC42 Rabbit Monoclonal antibody M00119 for rat tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
The anti-CDC42 Rabbit Monoclonal antibody (M00119) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-02
Question
Please see the WB image, lot number and protocol we used for placenta using anti-CDC42 Rabbit Monoclonal antibody M00119. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-02
Question
Do you have a BSA free version of anti-CDC42 Rabbit Monoclonal antibody M00119 available?
A. Roberts
Verified customer
Asked: 2019-07-04
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CDC42 Rabbit Monoclonal antibody M00119 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-04
Question
Our lab want to know about to test anti-CDC42 Rabbit Monoclonal antibody M00119 on mouse placenta for research purposes, then I may be interested in using anti-CDC42 Rabbit Monoclonal antibody M00119 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
The products we sell, including anti-CDC42 Rabbit Monoclonal antibody M00119, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-29
Question
We have tried in the past anti-CDC42 Rabbit Monoclonal antibody for IP on fetal brain a few months ago. I am using human, and I plan to use the antibody for WB next. I am looking for examining fetal brain as well as cervix in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
I looked at the website and datasheets of our anti-CDC42 Rabbit Monoclonal antibody and I see that M00119 has been tested on human in both IP and WB. Thus M00119 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-19
Question
Would M00119 anti-CDC42 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
You can see on the product datasheet, M00119 anti-CDC42 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-07
Question
I was wanting to use your anti-CDC42 Rabbit Monoclonal antibody for IP for mouse placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2018-09-25
Answer
You can see on the product datasheet, M00119 anti-CDC42 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-25
Question
Is this M00119 anti-CDC42 Rabbit Monoclonal antibody reactive to the isotypes of CDC42?
Verified Customer
Verified customer
Asked: 2018-06-21
Answer
The immunogen of M00119 anti-CDC42 Rabbit Monoclonal antibody is A synthesized peptide derived from human CDC42. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-21
Question
My colleagues were content with the WB result of your anti-CDC42 Rabbit Monoclonal antibody. However we have been able to see positive staining in neutrophil cell membrane using this antibody. Is that expected? Could you tell me where is CDC42 supposed to be expressed?
J. Carter
Verified customer
Asked: 2018-05-09
Answer
From literature, neutrophil does express CDC42. Generally CDC42 expresses in cell membrane. Regarding which tissues have CDC42 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 10490598
Fetal brain, Pubmed ID: 2122236
Neutrophil, Pubmed ID: 8504089
Placenta, Pubmed ID: 2124704
Boster Scientific Support
Answered: 2018-05-09
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-CDC42 Rabbit Monoclonal antibody M00119. Let me know if you need anything else.
F. Roberts
Verified customer
Asked: 2017-11-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-20
Question
We have seen staining in human placenta uterus. Any tips? Is anti-CDC42 Rabbit Monoclonal antibody supposed to stain placenta uterus positively?
N. Jones
Verified customer
Asked: 2017-06-26
Answer
Based on literature placenta uterus does express CDC42. Based on Uniprot.org, CDC42 is expressed in testis, fetal brain, placenta, brain placenta, cervix, placenta uterus, brain, cajal-retzius cell fetal brain cortex, neutrophil, embryo, among other tissues. Regarding which tissues have CDC42 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 10490598
Fetal brain, Pubmed ID: 2122236
Neutrophil, Pubmed ID: 8504089
Placenta, Pubmed ID: 2124704
Boster Scientific Support
Answered: 2017-06-26
Question
Does anti-CDC42 Rabbit Monoclonal antibody M00119 work for IP with placenta?
J. Jha
Verified customer
Asked: 2015-09-08
Answer
According to the expression profile of placenta, CDC42 is highly expressed in placenta. So, it is likely that anti-CDC42 Rabbit Monoclonal antibody M00119 will work for IP with placenta.
Boster Scientific Support
Answered: 2015-09-08
