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Product Info Summary
| SKU: | PA1366 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Bovine, Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CDC42 Antibody
SKU/Catalog Number
PA1366
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CDC42 Antibody catalog # PA1366. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDC42 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1366)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CDC42, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1366 is reactive to CDC42 in Bovine, Human, Mouse, Rat
Applications
PA1366 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
21259 MW
Background of CDC42
Cell division control protein 42 homolog also known as CDC42 is a protein involved in regulation of the cell cycle. In humans, CDC42 is encoded by the CDC42 gene. CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Bovine, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-CDC42 antibody, PA1366, Western blotting
Lane 1: Recombinant Human CDC42 Protein 10ng
Lane 2: Recombinant Human CDC42 Protein 5ng
Lane 3: Recombinant Human CDC42 Protein 2.5ng
Click image to see more details
Anti-CDC42 antibody, PA1366, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For CDC42 (Source: Uniprot.org, NCBI)
Gene Name
CDC42
Full Name
Cell division control protein 42 homolog
Weight
21259 MW
Superfamily
small GTPase superfamily
Alternative Names
CDC42; CDC42Hs; cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein, 25kDa); cell division cycle 42 (GTP-binding protein, 25kD); dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; G25K; growth-regulating protein; GTP-binding protein, 25kD; small GTP binding protein CDC42 CDC42 CDC42Hs, G25K, TKS cell division cycle 42 cell division control protein 42 homolog|G25K GTP-binding protein|GTP binding protein, 25kDa|dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD))|dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))|growth-regulating protein|small GTP binding protein CDC42
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC42, check out the CDC42 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC42: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDC42 Antibody (PA1366)
Hello CJ!
PA1366 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Han F1, Zhuang TT1, Chen JJ1, Zhu XL1,2, Cai YF1, Lu YP1. PLoS One. 2017 Sep 21;12(9):e0185102. doi: 10.1371/journal.pone.0185102. eCollection 2017. Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal ...
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-CDC42 Antibody
Question
I was wanting to use your anti-CDC42 antibody for WB for human brain placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain placenta identification?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
You can see on the product datasheet, PA1366 anti-CDC42 antibody has been tested for IHC, WB on bovine, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-24
Question
Is a blocking peptide available for product anti-CDC42 antibody (PA1366)?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We do provide the blocking peptide for product anti-CDC42 antibody (PA1366). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-28
Question
We have been able to see staining in mouse neutrophil. What should we do? Is anti-CDC42 antibody supposed to stain neutrophil positively?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
From what I have seen in literature neutrophil does express CDC42. From what I have seen in Uniprot.org, CDC42 is expressed in testis, fetal brain, placenta, brain placenta, cervix, placenta uterus, brain, cajal-retzius cell fetal brain cortex, neutrophil, embryo, among other tissues. Regarding which tissues have CDC42 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 10490598
Fetal brain, Pubmed ID: 2122236
Neutrophil, Pubmed ID: 8504089
Placenta, Pubmed ID: 2124704
Boster Scientific Support
Answered: 2020-01-30
Question
Is this PA1366 anti-CDC42 antibody reactive to the isotypes of CDC42?
J. Zhang
Verified customer
Asked: 2019-11-05
Answer
The immunogen of PA1366 anti-CDC42 antibody is A synthetic peptide corresponding to a sequence in the middle region of human CDC42(121-138aa DDPSTIEKLAKNKQKPIT), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-05
Question
Our team were well pleased with the WB result of your anti-CDC42 antibody. However we have seen positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is CDC42 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
From literature, placenta does express CDC42. Generally CDC42 expresses in cell membrane. Regarding which tissues have CDC42 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 10490598
Fetal brain, Pubmed ID: 2122236
Neutrophil, Pubmed ID: 8504089
Placenta, Pubmed ID: 2124704
Boster Scientific Support
Answered: 2019-10-08
Question
My question regarding product PA1366, anti-CDC42 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1366 anti-CDC42 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-05
Question
We ordered your anti-CDC42 antibody for WB on cajal-retzius cell fetal brain cortex in a previous project. I am using bovine, and We are going to use the antibody for IHC next. I am interested in examining cajal-retzius cell fetal brain cortex as well as brain placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
I looked at the website and datasheets of our anti-CDC42 antibody and it appears that PA1366 has been validated on bovine in both WB and IHC. Thus PA1366 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in bovine even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-27
Question
Is there a BSA free version of anti-CDC42 antibody PA1366 available?
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CDC42 antibody PA1366 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-11
Question
Will anti-CDC42 antibody PA1366 work on bovine WB with placenta?
L. Anderson
Verified customer
Asked: 2018-08-30
Answer
Our lab technicians have not validated anti-CDC42 antibody PA1366 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CDC42 antibody PA1366 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-30
Question
I see that the anti-CDC42 antibody PA1366 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-19
Question
Does PA1366 anti-CDC42 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
As indicated on the product datasheet, PA1366 anti-CDC42 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-29
Question
I am looking for using your anti-CDC42 antibody for cd28 dependent vav1 pathway studies. Has this antibody been tested with western blotting on mammary cancer tissue? We would like to see some validation images before ordering.
F. Anderson
Verified customer
Asked: 2016-05-03
Answer
I appreciate your inquiry. This PA1366 anti-CDC42 antibody is validated on mammary cancer tissue. It is guaranteed to work for IHC, WB in bovine, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-05-03
Question
My lab would like to test anti-CDC42 antibody PA1366 on human brain placenta for research purposes, then I may be interested in using anti-CDC42 antibody PA1366 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Mangal
Verified customer
Asked: 2015-05-26
Answer
The products we sell, including anti-CDC42 antibody PA1366, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-05-26
Question
Would anti-CDC42 antibody PA1366 work for WB with brain placenta?
L. Johnson
Verified customer
Asked: 2014-07-31
Answer
According to the expression profile of brain placenta, CDC42 is highly expressed in brain placenta. So, it is likely that anti-CDC42 antibody PA1366 will work for WB with brain placenta.
Boster Scientific Support
Answered: 2014-07-31
Question
Please see the WB image, lot number and protocol we used for brain placenta using anti-CDC42 antibody PA1366. Please let me know if you require anything else.
C. Anderson
Verified customer
Asked: 2014-06-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-06-16
Question
We are currently using anti-CDC42 antibody PA1366 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says bovine, human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
E. Kulkarni
Verified customer
Asked: 2013-11-07
Answer
The anti-CDC42 antibody (PA1366) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-07
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain placenta using anti-CDC42 antibody PA1366. Let me know if you need anything else.
S. Parker
Verified customer
Asked: 2013-07-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-07-30
