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Product Info Summary
| SKU: | M02236-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-CD8 alpha Rabbit Monoclonal Antibody
SKU/Catalog Number
M02236-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD8 alpha Rabbit Monoclonal Antibody catalog # M02236-2. Tested in IHC application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD8 alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02236-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BDG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD8 alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02236-2 is reactive to CD8A in Human
Applications
M02236-2 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
25729 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:50-1:200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody at 1:300 dilution.
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Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:300 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse testis, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human melanoma, using the Antibody at 1:300 dilution.
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Immunohistochemical analysis of paraffin-embedded human spleen, using CD8 alpha Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CD8A (Source: Uniprot.org, NCBI)
Gene Name
CD8A
Full Name
T-cell surface glycoprotein CD8 alpha chain
Weight
25729 MW
Alternative Names
53-6.7 CD8 Alpha; 53-6.7 CD8 Aplha; 53-6.7 CD8; 53-6.7 Clone; 53-6.7; CD8; CD8; CD8a molecule; CD8A; Cytotoxic T cell marker; Cytotoxic T lymphocyte marker; LEU2; p32; T cell marker CD8A CD8, Leu2, p32 CD8a molecule T-cell surface glycoprotein CD8 alpha chain|CD8 antigen, alpha polypeptide (p32)|Leu2 T-lymphocyte antigen|OKT8 T-cell antigen|T cell co-receptor|T-cell antigen Leu2|T-lymphocyte differentiation antigen T8/Leu-2|T8 T-cell antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD8A, check out the CD8A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD8A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD8 alpha Rabbit Monoclonal Antibody (M02236-2)
Hello CJ!
M02236-2 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-CD8 alpha Rabbit Monoclonal Antibody
Question
I see that the anti-CD8 alpha Rabbit Monoclonal antibody M02236-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-24
Question
We are currently using anti-CD8 alpha Rabbit Monoclonal antibody M02236-2 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The anti-CD8 alpha Rabbit Monoclonal antibody (M02236-2) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-06
Question
Is this M02236-2 anti-CD8 alpha Rabbit Monoclonal antibody reactive to the isotypes of CD8A?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
The immunogen of M02236-2 anti-CD8 alpha Rabbit Monoclonal antibody is A synthesized peptide derived from human CD8 alpha. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-27
Question
Our team were well pleased with the WB result of your anti-CD8 alpha Rabbit Monoclonal antibody. However we have observed positive staining in thymus isoform 2: secreted using this antibody. Is that expected? Could you tell me where is CD8A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
From literature, thymus does express CD8A. Generally CD8A expresses in isoform 1: cell membrane, isoform 2: secreted. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-23
Question
I was wanting to use your anti-CD8 alpha Rabbit Monoclonal antibody for IHC for human pericardium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pericardium identification?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
It shows on the product datasheet, M02236-2 anti-CD8 alpha Rabbit Monoclonal antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human pericardium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-19
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pericardium using anti-CD8 alpha Rabbit Monoclonal antibody M02236-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-13
Question
We are interested in to test anti-CD8 alpha Rabbit Monoclonal antibody M02236-2 on human pericardium for research purposes, then I may be interested in using anti-CD8 alpha Rabbit Monoclonal antibody M02236-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The products we sell, including anti-CD8 alpha Rabbit Monoclonal antibody M02236-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-09
Question
Is there a BSA free version of anti-CD8 alpha Rabbit Monoclonal antibody M02236-2 available?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD8 alpha Rabbit Monoclonal antibody M02236-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-28
Question
Please see the WB image, lot number and protocol we used for pericardium using anti-CD8 alpha Rabbit Monoclonal antibody M02236-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-06
Question
Does M02236-2 anti-CD8 alpha Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
It shows on the product datasheet, M02236-2 anti-CD8 alpha Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-02
Question
Is a blocking peptide available for product anti-CD8 alpha Rabbit Monoclonal antibody (M02236-2)?
Verified Customer
Verified customer
Asked: 2018-07-16
Answer
We do provide the blocking peptide for product anti-CD8 alpha Rabbit Monoclonal antibody (M02236-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-07-16
Question
Would anti-CD8 alpha Rabbit Monoclonal antibody M02236-2 work for IHC with pericardium?
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
According to the expression profile of pericardium, CD8A is highly expressed in pericardium. So, it is likely that anti-CD8 alpha Rabbit Monoclonal antibody M02236-2 will work for IHC with pericardium.
Boster Scientific Support
Answered: 2018-04-26
Question
Can you help my question with product M02236-2, anti-CD8 alpha Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02236-2 anti-CD8 alpha Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-02
Question
We have observed staining in human pericardium. Any tips? Is anti-CD8 alpha Rabbit Monoclonal antibody supposed to stain pericardium positively?
D. Johnson
Verified customer
Asked: 2017-08-01
Answer
From literature pericardium does express CD8A. From Uniprot.org, CD8A is expressed in thymus, pericardium, among other tissues. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-08-01
