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Product Info Summary
| SKU: | A02236-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Mouse CD8 alpha DyLight® 550 conjugated Antibody
SKU/Catalog Number
A02236-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Mouse CD8 alpha DyLight® 550 conjugated Antibody catalog # A02236-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Mouse.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Mouse CD8 alpha DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02236-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02236-Dyl550 is reactive to Cd8a in Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
25.729kDa
Background of CD8
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02236-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For Cd8a (Source: Uniprot.org, NCBI)
Gene Name
Cd8a
Full Name
T-cell surface glycoprotein CD8 alpha chain
Weight
25.729kDa
Alternative Names
T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; CD8a; Cd8a; Lyt-2; Lyt2 CD8A CD8, Leu2, p32 CD8a molecule T-cell surface glycoprotein CD8 alpha chain|CD8 , alpha polypeptide (p32)|Leu2 T-lymphocyte |OKT8 T-cell |T cell co-receptor|T-cell Leu2|T-lymphocyte differentiation T8/Leu-2|T8 T-cell
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd8a, check out the Cd8a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd8a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mouse CD8 alpha DyLight® 550 conjugated Antibody (A02236-Dyl550)
Hello CJ!
A02236-Dyl550 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Myeloid differentiation factor 88 promotes cisplatin chemoresistance in ovarian cancer
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Mouse CD8 alpha DyLight® 550 conjugated Antibody
Question
Is this A02236-Dyl550 anti-Mouse CD8 alpha DyLight® 550 conjugated antibody reactive to the isotypes of CD8A?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
The immunogen of A02236-Dyl550 anti-Mouse CD8 alpha DyLight® 550 conjugated antibody is E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-01
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-19
Question
Will anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 work for Flow Cytometry with thymus?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
According to the expression profile of thymus, CD8A is highly expressed in thymus. So, it is likely that anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 will work for Flow Cytometry with thymus.
Boster Scientific Support
Answered: 2019-08-29
Question
Does A02236-Dyl550 anti-Mouse CD8 alpha DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
You can see on the product datasheet, A02236-Dyl550 anti-Mouse CD8 alpha DyLight® 550 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-24
Question
Is there a BSA free version of anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 available?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-12
Question
We have observed staining in mouse thymus. Are there any suggestions? Is anti-Mouse CD8 alpha DyLight® 550 conjugated antibody supposed to stain thymus positively?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
From what I have seen in literature thymus does express CD8A. From what I have seen in Uniprot.org, CD8A is expressed in thymus, pericardium, among other tissues. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-10
Question
My boss were well pleased with the WB result of your anti-Mouse CD8 alpha DyLight® 550 conjugated antibody. However we have observed positive staining in pericardium isoform 2: secreted using this antibody. Is that expected? Could you tell me where is CD8A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
From what I have seen in literature, pericardium does express CD8A. Generally CD8A expresses in isoform 1: cell membrane, isoform 2: secreted. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-05-29
Question
I was wanting to use your anti-Mouse CD8 alpha DyLight® 550 conjugated antibody for Flow Cytometry for mouse thymus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse thymus identification?
Verified Customer
Verified customer
Asked: 2018-06-11
Answer
It shows on the product datasheet, A02236-Dyl550 anti-Mouse CD8 alpha DyLight® 550 conjugated antibody has been validated for Flow Cytometry on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-11
Question
I see that the anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-26
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-26
Question
Is a blocking peptide available for product anti-Mouse CD8 alpha DyLight® 550 conjugated antibody (A02236-Dyl550)?
Verified Customer
Verified customer
Asked: 2017-10-18
Answer
We do provide the blocking peptide for product anti-Mouse CD8 alpha DyLight® 550 conjugated antibody (A02236-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-18
Question
We are currently using anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 for mouse tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on feline tissues as well?
N. Jha
Verified customer
Asked: 2017-10-11
Answer
The anti-Mouse CD8 alpha DyLight® 550 conjugated antibody (A02236-Dyl550) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-11
Question
We are interested in to test anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 on mouse thymus for research purposes, then I may be interested in using anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Carter
Verified customer
Asked: 2017-07-10
Answer
The products we sell, including anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-10
Question
Please see the WB image, lot number and protocol we used for thymus using anti-Mouse CD8 alpha DyLight® 550 conjugated antibody A02236-Dyl550. Please let me know if you require anything else.
R. Wu
Verified customer
Asked: 2016-07-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-07-11
Question
I have a question about product A02236-Dyl550, anti-Mouse CD8 alpha DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Parker
Verified customer
Asked: 2016-03-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02236-Dyl550 anti-Mouse CD8 alpha DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-03-17
