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Product Info Summary
| SKU: | A02236-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD8 alpha/Cd8a Antibody Picoband®
SKU/Catalog Number
A02236-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD8 alpha/Cd8a Antibody Picoband® catalog # A02236-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD8 alpha/Cd8a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02236-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02236-1 is reactive to Cd8a in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
25.729kDa
Background of CD8
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02236-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat spleen tissue, rat thymus tissue
IHC: mouse spleen tissue, rat spleen tissue
FCM: mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD8 alpha using anti-CD8 alpha antibody (A02236-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysate,
Lane 2: rat thymus tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD8 alpha antigen affinity purified polyclonal antibody (Catalog # A02236-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD8 alpha at approximately 36KD. The expected band size for CD8 alpha is at 26KD.
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Figure 2. IHC analysis of CD8 alpha using anti-CD8 alpha antibody (A02236-1).
CD8 alpha was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD8 alpha Antibody (A02236-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CD8 alpha using anti-CD8 alpha antibody (A02236-1).
CD8 alpha was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD8 alpha Antibody (A02236-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Flow Cytometry analysis of mouse spleen tissues using anti-CD8 alpha antibody (A02236-1).
Overlay histogram showing mouse spleen tissues stained with A02236-1 (Blue line). The tissues were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD8 alpha Antibody (A02236-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Cd8a (Source: Uniprot.org, NCBI)
Gene Name
Cd8a
Full Name
T-cell surface glycoprotein CD8 alpha chain
Weight
25.729kDa
Alternative Names
T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; CD8a; Cd8a; Lyt-2; Lyt2 CD8A CD8, Leu2, p32 CD8a molecule T-cell surface glycoprotein CD8 alpha chain|CD8 , alpha polypeptide (p32)|Leu2 T-lymphocyte |OKT8 T-cell |T cell co-receptor|T-cell Leu2|T-lymphocyte differentiation T8/Leu-2|T8 T-cell
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd8a, check out the Cd8a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd8a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD8 alpha/Cd8a Antibody Picoband® (A02236-1)
Hello CJ!
A02236-1 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Berberine Prolongs Mouse Heart Allograft Survival by Activating T Cell Apoptosis via the Mitochondrial Pathway
Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation:
NO/ROS/RNS Cascaded-Releasing Nano-Platform for Gas/PDT/PTT/Immunotherapy of Tumor
Liu Q,Hao Y,Du R,Hu D,Xie J,Zhang J,Deng G,Liang N,Tian T,Käsmann L,Rades D,Rim CH,Hu P,Zhang J.Radiotherapy programs neutrophils to an antitumor phenotype by inducing mesenchymal-epithelial transition.Transl Lung Cancer Res.2021 Mar;10(3):1424-1443.doi:10.21037/tlcr-21-152.PMID:33889520;PMCID:PMC8044478.
Species: Mouse
A02236-1 usage in article: APP:IHC, SAMPLE:SPLEEN AND LYMPH NODES, DILUTION:1:400
Dai X,Lu L,Deng S,Meng J,Wan C,Huang J,Sun Y,Hu Y,Wu B,Wu G,Lovell JF,Jin H,Yang K.USP7 targeting modulates anti-tumor immune response by reprogramming Tumor-associated Macrophages in Lung Cancer.Theranostics.2020 Jul 23;10(20):9332-9347.doi:10.7150/thno.47137.PMID:32802195;PMCID:PMC7415808.
Species: Mouse
A02236-1 usage in article: APP:IF, SAMPLE:LUNG TISSUE, DILUTION:NA
Jia-Hui Yan,Yi-Shun Ji,Man-Li Yang,Jun Fu,Hu Shan,Li−Li Wang,Lei Zhang,Jing-Yuan Xiao,Xiao-Ying Kong, and Jin-Sheng Shi ACS Applied Nano Materials 2020 3 (9), 8817-8828 DOI: 10.1021/acsanm.0c01607
Species: Cuttlefish,Human,Mouse
A02236-1 usage in article: APP:IHC, SAMPLE:HEART,LIVER,SPLEEN,LUNG AND KIDNEY, DILUTION:NA
Myeloid differentiation factor 88 promotes cisplatin chemoresistance in ovarian cancer
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Zhao N, Li X, He X, Qiu Y, Zhu L, Qi F. J Gene Med. 2013 Oct;15(10):366-74. Doi: 10.1002/Jgm.2739. Interleukin-15 Gene Therapy And The Mammalian Target Of Rapamycin Inhibitor Everolimus Inhibit The Growth Of Metastatic Breast Cancer.
Wang X, Shi L, Tu Q, Wang H, Zhang H, Wang P, Zhang L, Huang Z, Zhao F, Luan H, Wang X. Int J Nanomedicine. 2015 Jan 6;10:347-55. Doi: 10.2147/Ijn.S71245. Ecollection 2015. Treating Cutaneous Squamous Cell Carcinoma Using 5-Aminolevulinic Acid Pol...
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-CD8 alpha/Cd8a Antibody Picoband®
Question
I am interested in to test anti-CD8 alpha/Cd8a antibody A02236-1 on rat thymus for research purposes, then I may be interested in using anti-CD8 alpha/Cd8a antibody A02236-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The products we sell, including anti-CD8 alpha/Cd8a antibody A02236-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-21
Question
We ordered your anti-CD8 alpha/Cd8a antibody for Flow Cytometry on thymus in a previous project. I am using rat, and We want to use the antibody for ELISA next. I would like examining thymus as well as pericardium in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
I took a look at the website and datasheets of our anti-CD8 alpha/Cd8a antibody and I see that A02236-1 has been validated on rat in both Flow Cytometry and ELISA. Thus A02236-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-14
Question
We have been able to see staining in mouse pericardium. Do you have any suggestions? Is anti-CD8 alpha/Cd8a antibody supposed to stain pericardium positively?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
From what I have seen in literature pericardium does express CD8A. From what I have seen in Uniprot.org, CD8A is expressed in thymus, pericardium, among other tissues. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-06
Question
My colleagues were content with the WB result of your anti-CD8 alpha/Cd8a antibody. However we have observed positive staining in pericardium isoform 2: secreted using this antibody. Is that expected? Could you tell me where is CD8A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
From literature, pericardium does express CD8A. Generally CD8A expresses in isoform 1: cell membrane, isoform 2: secreted. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-05-20
Question
Is a blocking peptide available for product anti-CD8 alpha/Cd8a antibody (A02236-1)?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
We do provide the blocking peptide for product anti-CD8 alpha/Cd8a antibody (A02236-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-13
Question
Can A02236-1 detect the extracellular domain of CD8-alpha which is composed of its Exon1 and 2 mainly?
Verified customer
Asked: 2019-03-27
Answer
Yes, the Anti-CD8 alpha/Cd8a Picoband Antibody (A02236-1) K28- Y196 Uniprot: P01731 is the antibody we suggest for the experiment.
Boster Scientific Support
Answered: 2019-03-29
Question
Will A02236-1 anti-CD8 alpha/Cd8a antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
W. Lewis
Verified customer
Asked: 2018-11-30
Answer
You can see on the product datasheet, A02236-1 anti-CD8 alpha/Cd8a antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-30
Question
See attached the WB image, lot number and protocol we used for thymus using anti-CD8 alpha/Cd8a antibody A02236-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-CD8 alpha/Cd8a antibody A02236-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-12
Question
I see that the anti-CD8 alpha/Cd8a antibody A02236-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-11-21
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-11-21
Question
We are currently using anti-CD8 alpha/Cd8a antibody A02236-1 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-18
Answer
The anti-CD8 alpha/Cd8a antibody (A02236-1) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-18
Question
I was wanting to use your anti-CD8 alpha/Cd8a antibody for ELISA for rat thymus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat thymus identification?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
You can see on the product datasheet, A02236-1 anti-CD8 alpha/Cd8a antibody has been validated for ELISA, Flow Cytometry, IHC, ICC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-26
Question
Is this A02236-1 anti-CD8 alpha/Cd8a antibody reactive to the isotypes of CD8A?
A. Jones
Verified customer
Asked: 2016-05-09
Answer
The immunogen of A02236-1 anti-CD8 alpha/Cd8a antibody is E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-05-09
Question
Do you have a BSA free version of anti-CD8 alpha/Cd8a antibody A02236-1 available?
M. Anderson
Verified customer
Asked: 2015-02-06
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD8 alpha/Cd8a antibody A02236-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-02-06
Question
Would anti-CD8 alpha/Cd8a antibody A02236-1 work for ELISA with thymus?
R. Roberts
Verified customer
Asked: 2015-01-05
Answer
According to the expression profile of thymus, CD8A is highly expressed in thymus. So, it is likely that anti-CD8 alpha/Cd8a antibody A02236-1 will work for ELISA with thymus.
Boster Scientific Support
Answered: 2015-01-05
Question
My question regarding product A02236-1, anti-CD8 alpha/Cd8a antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Li
Verified customer
Asked: 2014-04-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02236-1 anti-CD8 alpha/Cd8a antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-04-14
Question
I would like using your anti-CD8 alpha/Cd8a antibody for defense response to virus studies. Has this antibody been tested with western blotting on spleen tissue? We would like to see some validation images before ordering.
K. Bhatt
Verified customer
Asked: 2013-10-22
Answer
We appreciate your inquiry. This A02236-1 anti-CD8 alpha/Cd8a antibody is validated on rat thymus tissue, spleen tissue, tissue lysate, mouse spleen tissue. It is guaranteed to work for ELISA, Flow Cytometry, IHC, ICC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-10-22
