Click image to see more details
Product Info Summary
| SKU: | PB9249 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD8 alpha/CD8A Antibody Picoband®
SKU/Catalog Number
PB9249
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD8 alpha/CD8A Antibody Picoband® catalog # PB9249. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD8 alpha/CD8A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9249)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD8 alpha recombinant protein (Position: S22-A187). Human CD8 alpha shares 49% and 47% amino acid (aa) sequence identity with mouse and rat CD8 alpha, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9249 is reactive to CD8A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
25729 MW
Background of CD8
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9249 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: JURKAT Whole Cell, RAJI Whole Cell, HL-60 Whole Cell, A549 Whole Cell, COLO320 Whole Cell, HELA Whole Cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD 8 alpha using anti-CD 8 alpha antibody (PB9249).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: JURKAT Whole Cell Lysate,
Lane 2: RAJI Whole Cell Lysate,
Lane 3: HL-60 Whole Cell Lysate,
Lane 4: A549 Whole Cell Lysate,
Lane 5: COLO320 Whole Cell Lysate,
Lane 6: HELA Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD 8 alpha antigen affinity purified polyclonal antibody (Catalog # PB9249) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD 8 alpha at approximately 55 kDa. The expected band size for CD 8 alpha is at 26 kDa.
Click image to see more details
Figure 2. IHC analysis of CD8 alpha using anti-CD8 alpha antibody (PB9249).
CD8 alpha was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD8 alpha Antibody (PB9249) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CD8A (Source: Uniprot.org, NCBI)
Gene Name
CD8A
Full Name
T-cell surface glycoprotein CD8 alpha chain
Weight
25729 MW
Alternative Names
T-cell surface glycoprotein CD8 alpha chain;T-lymphocyte differentiation antigen T8/Leu-2;CD8a;CD8A;MAL; CD8A CD8, Leu2, p32 CD8a molecule T-cell surface glycoprotein CD8 alpha chain|CD8 , alpha polypeptide (p32)|Leu2 T-lymphocyte |OKT8 T-cell |T cell co-receptor|T-cell Leu2|T-lymphocyte differentiation T8/Leu-2|T8 T-cell
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD8A, check out the CD8A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD8A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD8 alpha/CD8A Antibody Picoband® (PB9249)
Hello CJ!
PB9249 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
MicroRNA-421 mediates immunosensitivity in late-stage human liver cancer
Clinical biocharacterization of immunophenotype in hepatocellular carcinoma patients
Effect of l-arginine on intestinal mucosal immune barrier function in weaned pigs after Escherichia coli LPS challenge:
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Xu Q, Chen Y, Zhao Wm, Huang Zy, Duan Xj, Tong Yy, Zhang Y, Li X, Chang Gb, Chen Gh. Mol Biol Rep. 2015 Feb;42(2):431-9. Doi: 10.1007/S11033-014-3784-3. Epub 2014 Oct 21. The Cd8?? Gene In Duck (Anatidae): Cloning, Characterization, And Expression...
Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...
Chen X, Wan J, Liu J, Xie W, Diao X, Xu J, Zhu B, Chen Z. Lung Cancer. 2010 Sep;69(3):348-54. Doi: 10.1016/J.Lungcan.2009.11.013. Increased Il-17-Producing Cells Correlate With Poor Survival And Lymphangiogenesis In Nsclc Patients.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD8 alpha/CD8A Antibody Picoband®
Question
I see that the anti-CD8 alpha/CD8A antibody PB9249 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-19
Question
Would anti-CD8 alpha/CD8A antibody PB9249 work for WB with thymus?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
According to the expression profile of thymus, CD8A is highly expressed in thymus. So, it is likely that anti-CD8 alpha/CD8A antibody PB9249 will work for WB with thymus.
Boster Scientific Support
Answered: 2019-10-17
Question
We have seen staining in human pericardium. Do you have any suggestions? Is anti-CD8 alpha/CD8A antibody supposed to stain pericardium positively?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
From what I have seen in literature pericardium does express CD8A. From what I have seen in Uniprot.org, CD8A is expressed in thymus, pericardium, among other tissues. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-04
Question
My team were satisfied with the WB result of your anti-CD8 alpha/CD8A antibody. However we have been able to see positive staining in pericardium isoform 2: secreted using this antibody. Is that expected? Could you tell me where is CD8A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
From what I have seen in literature, pericardium does express CD8A. Generally CD8A expresses in isoform 1: cell membrane, isoform 2: secreted. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-26
Question
I was wanting to use using your anti-CD8 alpha/CD8A antibody for antigen processing and presentation studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Thanks for your inquiry. This PB9249 anti-CD8 alpha/CD8A antibody is validated on human tonsil tissue, jurkat whole cell lysate, raji whole cell lysate, a549 whole cell lysate, colo320 whole cell lysate, hela whole cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-18
Question
Is there a BSA free version of anti-CD8 alpha/CD8A antibody PB9249 available?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD8 alpha/CD8A antibody PB9249 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-CD8 alpha/CD8A antibody PB9249. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-13
Question
Does PB9249 anti-CD8 alpha/CD8A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
You can see on the product datasheet, PB9249 anti-CD8 alpha/CD8A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-31
Question
Is a blocking peptide available for product anti-CD8 alpha/CD8A antibody (PB9249)?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
We do provide the blocking peptide for product anti-CD8 alpha/CD8A antibody (PB9249). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-28
Question
Here is the WB image, lot number and protocol we used for thymus using anti-CD8 alpha/CD8A antibody PB9249. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-05-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-19
Question
Is this PB9249 anti-CD8 alpha/CD8A antibody reactive to the isotypes of CD8A?
K. Jha
Verified customer
Asked: 2017-05-12
Answer
The immunogen of PB9249 anti-CD8 alpha/CD8A antibody is E.coli-derived human CD8 alpha recombinant protein (Position: S22-A187). Human CD8 alpha shares 49% and 47% amino acid (aa) sequence identity with mouse and rat CD8 alpha, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-12
Question
We purchased anti-CD8 alpha/CD8A antibody for WB on pericardium in a previous project. I am using human, and We want to use the antibody for IHC next. My lab would like examining pericardium as well as thymus in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
L. Yang
Verified customer
Asked: 2017-05-08
Answer
I took a look at the website and datasheets of our anti-CD8 alpha/CD8A antibody and it seems that PB9249 has been tested on human in both WB and IHC. Thus PB9249 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-05-08
Question
My question regarding product PB9249, anti-CD8 alpha/CD8A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Moore
Verified customer
Asked: 2014-10-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9249 anti-CD8 alpha/CD8A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-10-27
Question
We want to test anti-CD8 alpha/CD8A antibody PB9249 on human thymus for research purposes, then I may be interested in using anti-CD8 alpha/CD8A antibody PB9249 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Edwards
Verified customer
Asked: 2014-01-10
Answer
The products we sell, including anti-CD8 alpha/CD8A antibody PB9249, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-01-10
Question
We are currently using anti-CD8 alpha/CD8A antibody PB9249 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
R. Krishna
Verified customer
Asked: 2013-06-18
Answer
The anti-CD8 alpha/CD8A antibody (PB9249) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-18
Question
I was wanting to use your anti-CD8 alpha/CD8A antibody for WB for human thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thymus identification?
F. Li
Verified customer
Asked: 2013-02-15
Answer
You can see on the product datasheet, PB9249 anti-CD8 alpha/CD8A antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-02-15
