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Product Info Summary
| SKU: | MA1017 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, ICC |
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Product info
Product Name
Anti-CD8 Cd8A Antibody (Monoclonal, CA-8)
SKU/Catalog Number
MA1017
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD8 Cd8A Antibody (Monoclonal, CA-8) catalog # MA1017. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD8 Cd8A Antibody (Monoclonal, CA-8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1017)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
CA-8
Isotype
Mouse IgG2a
Immunogen
Human thymocytes followed by peripheral blood T cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1017 is reactive to CD8A in Human
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
23400 MW
Background of CD8
The human lymphocyte differentiation antigen CD8 is encoded by a single gene that gives rise to a 33- to 34-kDa glycoprotein expressed on the cell surface as a dimer and in higher molecular mass forms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1017 is guaranteed for Flow Cytometry, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Positive Control
IHC: human tonsil tissue
FCM: human PBMC cell
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD8 antibody (MA1017).
Overlay histogram showing human PBMC cells stained with MA1017 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD8 Antibody (MA1017,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 2. IHC analysis of CD8 using anti-CD8 antibody (MA1017).
CD8 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD8 Antibody (MA1017) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CD8A (Source: Uniprot.org, NCBI)
Gene Name
CD8A
Full Name
T-cell surface glycoprotein CD8 alpha chain
Weight
23400 MW
Alternative Names
T-cell surface glycoprotein CD8 beta chain;CD8 antigen 37 kDa chain;OX-8 membrane antigen;CD8b;Cd8b;Cd8b1; CD8A CD8, Leu2, p32 CD8a molecule T-cell surface glycoprotein CD8 alpha chain|CD8 , alpha polypeptide (p32)|Leu2 T-lymphocyte |OKT8 T-cell |T cell co-receptor|T-cell Leu2|T-lymphocyte differentiation T8/Leu-2|T8 T-cell
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD8A, check out the CD8A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD8A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD8 Cd8A Antibody (Monoclonal, CA-8) (MA1017)
Hello CJ!
MA1017 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu Q, Chen Y, Zhao Wm, Huang Zy, Duan Xj, Tong Yy, Zhang Y, Li X, Chang Gb, Chen Gh. Mol Biol Rep. 2015 Feb;42(2):431-9. Doi: 10.1007/S11033-014-3784-3. Epub 2014 Oct 21. The Cd8?? Gene In Duck (Anatidae): Cloning, Characterization, And Expression...
Zhao N, Li X, He X, Qiu Y, Zhu L, Qi F. J Gene Med. 2013 Oct;15(10):366-74. Doi: 10.1002/Jgm.2739. Interleukin-15 Gene Therapy And The Mammalian Target Of Rapamycin Inhibitor Everolimus Inhibit The Growth Of Metastatic Breast Cancer.
Wang X, Shi L, Tu Q, Wang H, Zhang H, Wang P, Zhang L, Huang Z, Zhao F, Luan H, Wang X. Int J Nanomedicine. 2015 Jan 6;10:347-55. Doi: 10.2147/Ijn.S71245. Ecollection 2015. Treating Cutaneous Squamous Cell Carcinoma Using 5-Aminolevulinic Acid Pol...
Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...
Wu Y, Wang Y, Qi X, Zhang P, Zhang C, Zhang W. Nephron Exp Nephrol. 2014;128(1-2):46-56. Doi: 10.1159/000366446. Epub 2014 Nov 5. Increased Macrophage Activation Inhibited By Tacrolimus In The Kidney Of Diabetic Rats.
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-CD8 Cd8A Antibody (Monoclonal, CA-8)
Question
Has MA1017 been tested with IHC-P? What is the immunogen information?
Verified customer
Asked: 2019-07-09
Answer
The Anti-CD8 Cd8b Antibody (Monoclonal, CA-8) (MA1017) has been tested with IHC-P on human samples. It hasn't been validated with monkey samples. Please run pilot tests with monkey samples. Immunogen information: Human thymocytes followed by peripheral blood T cells.
Boster Scientific Support
Answered: 2019-07-10
Question
I would like using your anti-CD8 antibody (Monoclonal, CA-8) for adaptive immune response studies. Has this antibody been tested with western blotting on human pbmc cells? We would like to see some validation images before ordering.
T. Carter
Verified customer
Asked: 2013-10-17
Answer
I appreciate your inquiry. This MA1017 anti-CD8 antibody (Monoclonal, CA-8) is validated on human pbmc cells. It is guaranteed to work for Flow Cytometry, IHC, ICC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-10-17
