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Product Info Summary
| SKU: | PB9363 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-CD62P/SELP Antibody Picoband®
View all P-Selectin/CD62P Antibodies
SKU/Catalog Number
PB9363
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD62P/SELP Antibody Picoband® catalog # PB9363. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD62P/SELP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9363)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD62P recombinant protein (Position: W42-G271). Human CD62P shares 75.2% and 75.7% amino acid (aa) sequence identity with mouse and rat CD62P, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9363 is reactive to SELP in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
91 kDa
Calculated molecular weight
90834 MW
Background of P-Selectin/CD62P
CD62P is also known as SELP or P-selectin. This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9363 is guaranteed for ELISA, Flow Cytometry, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Positive Control
WB: A549 Whole Cell, K562 Whole Cell
IHC: Human Tonsil tissue
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. IHC analysis of CD62P using anti-CD62P antibody (PB9363).
CD62P was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD62P Antibody (PB9363) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. Western blot analysis of CD62P using anti-CD62P antibody (PB9363).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 Whole Cell Lysate,
Lane 2: K562 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD62P antigen affinity purified polyclonal antibody (Catalog # PB9363) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD62P at approximately 91KD. The expected band size for CD62P is at 140KD.
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Figure 3. Flow Cytometry analysis of THP-1 cells using anti-P-Selectin antibody (PB9363).
Overlay histogram showing THP-1 cells stained with PB9363 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P-Selectin Antibody (PB9363,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For SELP (Source: Uniprot.org, NCBI)
Gene Name
SELP
Full Name
P-selectin
Weight
90834 MW
Superfamily
selectin/LECAM family
Alternative Names
P-selectin;CD62 antigen-like family member P;Granule membrane protein 140;GMP-140;Leukocyte-endothelial cell adhesion molecule 3;LECAM3;Platelet activation dependent granule-external membrane protein;PADGEM;CD62P;SELP;GMRP, GRMP; SELP CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL selectin P P-selectin|CD62 -like family member P|GMP-140|granule membrane protein 140|granule membrane protein 140kDa|granulocyte membrane protein|leukocyte-endothelial cell adhesion molecule 3|platelet activation dependent granule-external membrane protein|platelet alpha-granule membrane protein|selectin P (granule membrane protein 140kDa, CD62)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SELP, check out the SELP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SELP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD62P/SELP Antibody Picoband® (PB9363)
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1 Customer Q&As for Anti-CD62P/SELP Antibody Picoband®
Question
We are currently using anti-CD62P/SELP antibody PB9363 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The anti-CD62P/SELP antibody (PB9363) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-25
