Anti-Flotillin 2/FLOT2 Antibody Picoband®

Flotillin 2 antibody

Boster Bio Anti-Flotillin 2/FLOT2 Antibody Picoband® catalog # PB9623. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9623
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

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Product Name

Anti-Flotillin 2/FLOT2 Antibody Picoband®

View all Flotillin 2 Antibodies

SKU/Catalog Number

PB9623

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Flotillin 2/FLOT2 Antibody Picoband® catalog # PB9623. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Flotillin 2/FLOT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9623)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Flotillin 2 recombinant protein (Position: K169-K344). Human Flotillin 2 shares 100% amino acid (aa) sequence identity with both mouse and rat Flotillin 2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9623 is reactive to FLOT2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

47-50 kDa

Calculated molecular weight

47064 MW

Background of Flotillin 2

FLOT2 (Flotillin 2), also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al. (1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the ESA cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al. (1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al. (2012) found that palmitoylation and oligomerization of flotillin-2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of flotillin-2 was not changed in Dhhc5 mutant neurons.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9623 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells

Positive Control

WB: human 293T whole cell, human Hela whole cell, human A549 whole cell, rat lung tissue, mouse lung tissue
IHC: human mammary cancer tissue, mouse intestine tissue, rat kidney tissue
IHC-F: rat kidney tissue, rat small intestine tissue, mouse kidney tissue
ICC/IF: A431 cell
FCM: RH35 cell, U20S cell

Validation Images & Assay Conditions

Gene/Protein Information For FLOT2 (Source: Uniprot.org, NCBI)

Gene Name

FLOT2

Full Name

Flotillin-2

Weight

47064 MW

Superfamily

band 7/mec-2 family

Alternative Names

Flotillin-2;Epidermal surface antigen;ESA;Membrane component chromosome 17 surface marker 1;FLOT2;ESA1, M17S1; FLOT2 ECS-1, ECS1, ESA, ESA1, M17S1 flotillin 2 flotillin-2|Flotillin 2 (epidermal surface 1)|epidermal surface |membrane component chromosome 17 surface marker 1|membrane component, chromosome 17, surface marker 1 (35kD protein by ECS-1)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FLOT2, check out the FLOT2 Infographic

FLOT2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FLOT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Flotillin 2/FLOT2 Antibody Picoband®

Question

We are currently using anti-Flotillin 2/FLOT2 antibody PB9623 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

E. Li

Verified customer

Asked: 2019-08-26

Answer

The anti-Flotillin 2/FLOT2 antibody (PB9623) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-26

Order DetailsPrice
PB9623

100μg

$370
PB9623-10ug

10μg sample (liquid)

$99
PB9623-Biotin

100 μg Biotin conjugated

$570
PB9623-Cy3

100 μg Cy3 conjugated

$570
PB9623-Dylight488

100 μg Dylight488 conjugated

$570
PB9623-Dylight550

100 μg Dylight550 conjugated

$570
PB9623-Dylight594

100 μg Dylight594 conjugated

$570
PB9623-FITC

100 μg FITC conjugated

$570
PB9623-HRP

100 μg HRP conjugated

$570
PB9623-APC

100 μg APC conjugated

$670
PB9623-PE

100 μg PE conjugated

$670
PB9623-iFluor647

100 μg iFluor647 conjugated

$670
PB9623-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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