Anti-Flotillin 2/FLOT2 Picoband™ Antibody

SKU PB9623
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-Flotillin 2/FLOT2 Picoband™ Antibody
SKU/Catalog Number PB9623
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Flotillin-2(FLOT2) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
Cite This Product Anti-Flotillin 2/FLOT2 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9623)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Form Lyophilized
Immunogen E.coli-derived human Flotillin 2 recombinant protein (Position: K169-K344). Human Flotillin 2 shares 100% amino acid (aa) sequence identity with both mouse and rat Flotillin 2.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml
Western blot, 0.1-0.5μg/ml

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).

Images And Assay Conditions

Anti- Flotillin 2 Picoband antibody

Anti- Flotillin 2 Picoband antibody, PB9623, IHC(P)
IHC(P): Mouse Intestine Tissue

Anti- Flotillin 2 Picoband antibody, PB9623, IHC(P)
IHC(P): Rat Kidney Tissue

Anti- Flotillin 2 Picoband antibody, PB9623, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Figure 5. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623).
Flotillin 2 was detected in frozen section of mouse kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml r

Figure 6. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623).
Flotillin 2 was detected in frozen section of rat kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rab

Figure 7. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623).
Flotillin 2 was detected in frozen section of rat small intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q14254
Gene Name FLOT2
Protein Name Flotillin-2
Tissue Specificity In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
Alternative Names Flotillin-2;Epidermal surface antigen;ESA;Membrane component chromosome 17 surface marker 1;FLOT2;ESA1, M17S1;
Subcellular Localization Cell membrane ; Peripheral membrane protein . Membrane, caveola ; Peripheral membrane protein . Endosome . Membrane-associated protein of caveolae.
Molecular Weight 47064 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background FLOT2 (Flotillin 2), also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al. (1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the ESA cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al. (1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al. (2012) found that palmitoylation and oligomerization of flotillin-2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of flotillin-2 was not changed in Dhhc5 mutant neurons.

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In stock
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PB9623
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
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