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Product Info Summary
| SKU: | M00637 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-PAI1 Rabbit Monoclonal Antibody
View all Serpin E1/PAI-1 Antibodies
SKU/Catalog Number
M00637
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PAI1 Rabbit Monoclonal Antibody catalog # M00637. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAI1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00637)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABHG-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PAI1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00637 is reactive to SERPINE1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
85 kDa
Calculated molecular weight
45060 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00637 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
ICC/IF 1:50-1:200
IP 1:30
FC 1:50
Positive Control
WB: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of PAI1 expression in HepG2 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For SERPINE1 (Source: Uniprot.org, NCBI)
Gene Name
SERPINE1
Full Name
Plasminogen activator inhibitor 1
Weight
45060 MW
Superfamily
serpin family
Alternative Names
Plasminogen activator inhibitor 1;PAI;PAI-1;Endothelial plasminogen activator inhibitor;Serpin E1;SERPINE1;PAI1, PLANH1; SERPINE1 PAI, PAI-1, PAI1, PLANH1 serpin family E member 1 plasminogen activator inhibitor 1|endothelial plasminogen activator inhibitor|serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1|serpin E1|serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPINE1, check out the SERPINE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPINE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAI1 Rabbit Monoclonal Antibody (M00637)
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Customer Q&As
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4 Customer Q&As for Anti-PAI1 Rabbit Monoclonal Antibody
Question
Does M00637 anti-PAI1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
It shows on the product datasheet, M00637 anti-PAI1 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-06
Question
Is this M00637 anti-PAI1 Rabbit Monoclonal antibody reactive to the isotypes of SERPINE1?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The immunogen of M00637 anti-PAI1 Rabbit Monoclonal antibody is A synthesized peptide derived from human PAI1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-03
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-PAI1 Rabbit Monoclonal antibody M00637. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-02
Question
Do you have a BSA free version of anti-PAI1 Rabbit Monoclonal antibody M00637 available?
B. Singh
Verified customer
Asked: 2019-04-22
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PAI1 Rabbit Monoclonal antibody M00637 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-22
