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Product Info Summary
| SKU: | EK0505 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human P-Selectin / CD62P ELISA Kit PicoKine®
View all P-Selectin/CD62P ELISA kits
SKU/Catalog Number
EK0505
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human P-Selectin / CD62P ELISA Kit PicoKine® (96 Tests). Quantitate Human SELP in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human P-Selectin / CD62P ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0505)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: W42-A771
Sensitivity
<5 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0505 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0505 is reactive to SELP in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0505 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of P-Selectin/CD62P
P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1). The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0505-CAP | Anti-Human SELP Pre-coated 96-well strip microplate | 1 |
| EK0505-ST | Human SELP Standard | 2 vials, 10 ng/tube |
| EK0505-DA | Human SELP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.033 | 0.139 | 0.273 | 0.474 | 0.728 | 1.264 | 1.714 | 2.099 |
Data Example Images
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Human P-Selectin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect P-Selectin/CD62P, Dilution ratio of 1:20, concentration in serum and plasma is 51-113 ng/ml..
Some articles we found to cite concentrations of P-Selectin/CD62P in samples: 25218335 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human P-Selectin / CD62P ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 322 | 1820 | 5020 | 331 | 1849 | 5389 |
| Standard deviation | 15.63 | 121.94 | 256.02 | 21.18 | 144.22 | 301.78 |
| CV (%) | 4.7% | 6.7% | 5.6% | 6.4% | 7.8% | 5.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of P-Selectin/CD62P in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 322 | 316 | 323 | 357 | 329 | 16.1 | 4.8% |
| Sample 2 | 1820 | 1756 | 2051 | 1775 | 1850 | 118.06 | 6.3% |
| Sample 3 | 5020 | 5825 | 5171 | 5716 | 5433 | 343.86 | 6.3% |
Protein Target Info & Infographic
Gene/Protein Information For SELP (Source: Uniprot.Org, NCBI)
Gene Name
SELP
Full Name
P-selectin
Weight
90834 MW
Superfamily
selectin/LECAM family
Alternative Names
P-selectin;CD62 antigen-like family member P;Granule membrane protein 140;GMP-140;Leukocyte-endothelial cell adhesion molecule 3;LECAM3;Platelet activation dependent granule-external membrane protein;PADGEM;CD62P;SELP;GMRP, GRMP; SELP CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL selectin P P-selectin|CD62 -like family member P|GMP-140|granule membrane protein 140|granule membrane protein 140kDa|granulocyte membrane protein|leukocyte-endothelial cell adhesion molecule 3|platelet activation dependent granule-external membrane protein|platelet alpha-granule membrane protein|selectin P (granule membrane protein 140kDa, CD62)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SELP, check out the SELP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SELP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human P-Selectin / CD62P ELISA Kit PicoKine® (EK0505)
Hello CJ!
EK0505 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Nesibe,E.Y.,& KONUKOĞLU,D.(2021).Plasma Levels of Soluble P-Selectin, Beta-Thromboglobulin and Platelet Indices in Patients With Prediabetes: Effects of Acute Hyperglycemic Stress.Turkish Journal of Endocrinology and Metabolism,25(2),174.
Species: Human
EK0505 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA
Plasma Vitamin D Status and Its Correlation with Risk Factors of Thrombosis, P-selectin and hs-CRP Level in Patients with Venous Thromboembolism; the First Study of Iranian Population
Fayed, M.A., Abdel-Hady, H.E., Hafez, M.M., Salama, O.S., & Al-Tonbary, Y.A. (2017). Study of platelet activation, hypercoagulable state, and the association with pulmonary hypertension in children with β-thalassemia. Hematology/Oncology and Stem ...
Li L, Ben Y, Yuan S, Liu A, Wu H, Xu J, Zhang X. Emerg Microbes Infect. 2013 Jul;2(7):E42. Doi: 10.1038/Emi.2013.42. Epub 2013 Jul 10. Recruitment Of Hiv-1 Target Cells At Topical Mucosal Sites: A Sensitive And Early Marker For Determining The Saf...
Zhong M, He B, Yang J, Bao R, Zhang Y, Zhou D, Chen Y, Li L, Han C, Yang Y, Sun Y, Cao Y, Li Y, Shi W, Jiang S, Zhang X, Yan H. Antimicrob Agents Chemother. 2012 Jun;56(6):3121-32. Doi: 10.1128/Aac.05950-11. Epub 2012 Mar 5. L-Selectin And P-Selec...
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Human P-Selectin / CD62P ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for CD62P ELISA kit? I used your CD62P ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD62P even though the O.D. values are not very high?
V. Turner
Verified customer
Asked: 2020-04-21
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD62P ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of CD62P that can be declared/interpreted as positive by the above standard is 5pg/ml.
Boster Scientific Support
Answered: 2020-04-21
Question
Q: how to proceed with the analysis of ELISA data? I have obtained CD62P level in plasma.
L. Chakrabarti
Verified customer
Asked: 2019-06-24
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-06-24
Question
Does this kit detect total P-Selectin or soluble P-Selectin?
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
This kit (SKU: EK0505) is specific to soluble P-selectin.
Boster Scientific Support
Answered: 2018-11-22
Question
Q: Can CD62P ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Scott
Verified customer
Asked: 2018-09-29
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-09-29
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD62P in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-08-25
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD62P ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-08-25
Question
Q: if the enzyme conjugated CD62P antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD62P antigen?
Verified Customer
Verified customer
Asked: 2016-02-26
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-02-26
