Click image to see more details
Product Info Summary
| SKU: | PA1344 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-uPA Receptor/PLAUR Antibody Picoband®
SKU/Catalog Number
PA1344
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-uPA Receptor/PLAUR Antibody catalog # PA1344. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-uPA Receptor/PLAUR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1344)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human uPA Receptor.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1344 is reactive to PLAUR in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45-55 kDa
Calculated molecular weight
36978 MW
Background of uPAR
PLAUR (PLASMINOGEN ACTIVATOR RECEPTOR, UROKINASE-TYPE), also known as UPAR or CD87, is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. PLAUR consists of three different domains of the Ly-6/uPAR/alpha-neurotoxin family. PLAUR is originally identified as a saturable binding site for urokinase on the cell surface. And the gene plays an important role in many normal as well as pathologic processes. The PLAUR gene is localized to 19q13.31. PLAUR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. PLAUR binds urokinase and thus restricts plasminogen activation to the immediate vicinity of the cell membrane. Thus it seems to be an important player in the regulation of this process. In human coronary artery vascular smooth muscle cells, UPA stimulates cell migration via a UPAR signaling complex containing TYK2 and phosphatidylinositol 3-kinase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1344 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Positive Control
WB: human Hela whole cell
IHC: human glioma tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PLAUR using anti-PLAUR antibody (PA1344).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAUR antigen affinity purified polyclonal antibody (Catalog # PA1344) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAUR at approximately 45-55 kDa. The expected band size for PLAUR is at 37 kDa.
Click image to see more details
Figure 2. IHC analysis of PLAUR using anti-PLAUR antibody (PA1344).
PLAUR was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLAUR Antibody (PA1344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PLAUR (Source: Uniprot.org, NCBI)
Gene Name
PLAUR
Full Name
Urokinase plasminogen activator surface receptor
Weight
36978 MW
Alternative Names
Urokinase plasminogen activator surface receptor;U-PAR;uPAR;Monocyte activation antigen Mo3;CD87;PLAUR;MO3, UPAR; PLAUR CD87, U-PAR, UPAR, URKR plasminogen activator, urokinase receptor urokinase plasminogen activator surface receptor|monocyte activation Mo3|u-plasminogen activator receptor form 2|urokinase-type plasminogen activator (uPA) receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLAUR, check out the PLAUR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLAUR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-uPA Receptor/PLAUR Antibody Picoband® (PA1344)
Hello CJ!
PA1344 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Apigenin Suppresses the IL-1β-Induced Expression of the Urokinase-Type Plasminogen Activator Receptor by Inhibiting MAPK-Mediated AP-1 and NF-κB Signaling in Human Bladder Cancer T24 Cells
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-uPA Receptor/PLAUR Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-uPA Receptor/PLAUR Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-uPA Receptor/PLAUR Antibody Picoband®
Question
My question regards to test anti-uPA Receptor/PLAUR antibody PA1344 on human serum for research purposes, then I may be interested in using anti-uPA Receptor/PLAUR antibody PA1344 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
The products we sell, including anti-uPA Receptor/PLAUR antibody PA1344, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-21
Question
I see that the anti-uPA Receptor/PLAUR antibody PA1344 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-16
Question
Is this PA1344 anti-uPA Receptor/PLAUR antibody reactive to the isotypes of PLAUR?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
The immunogen of PA1344 anti-uPA Receptor/PLAUR antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human uPA Receptor(293-304aa CNHPDLDVQYRS). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-21
Question
Can you help my question with product PA1344, anti-uPA Receptor/PLAUR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1344 anti-uPA Receptor/PLAUR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-19
Question
Will PA1344 anti-uPA Receptor/PLAUR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-18
Answer
It shows on the product datasheet, PA1344 anti-uPA Receptor/PLAUR antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-18
Question
We are currently using anti-uPA Receptor/PLAUR antibody PA1344 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
A. Anderson
Verified customer
Asked: 2013-03-08
Answer
The anti-uPA Receptor/PLAUR antibody (PA1344) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-08
