Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine®

Product Name

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine®

View all uPAR ELISA kits

SKU/Catalog Number

EK0536

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine® (96 Tests). Quantitate Human PLAUR in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0536)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: L23-R303

Sensitivity

<4 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

See datasheet of EK0536 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0536 is reactive to PLAUR in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK0536 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of uPAR

The urokinase-type plasminogen activator receptor (uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes. The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator. UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer. PLAUR is located at chromosome 19q13.1-q13.2. The standard product used in this kit is recombinant human uPAR, consisting of 287 amino acids with the molecular mass of 31KDa.

Kit Components

Catalog Number	Description	Quantity
EK0536-CAP	Anti-Human PLAUR Pre-coated 96-well strip microplate	1
EK0536-ST	Human PLAUR Standard	2 vials, 10 ng/tube
EK0536-DA	Human PLAUR Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.025	0.085	0.164	0.345	0.602	1.078	1.536	2.011

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect uPAR, Dilution ratio of 1:10, concentration in serum is 2440-3406 pg/ml..

Some articles we found to cite concentrations of uPAR in samples: 24833530 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of uPAR in ELISA kits from four different production batches/lots.

Gene/Protein Information For PLAUR (Source: Uniprot.Org, NCBI)

Alternative Names

Urokinase plasminogen activator surface receptor;U-PAR;uPAR;Monocyte activation antigen Mo3;CD87;PLAUR;MO3, UPAR; PLAUR CD87, U-PAR, UPAR, URKR plasminogen activator, urokinase receptor urokinase plasminogen activator surface receptor|monocyte activation Mo3|u-plasminogen activator receptor form 2|urokinase-type plasminogen activator (uPA) receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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