Click image to see more details
Product Info Summary
| SKU: | A00068-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CD40/Cd40 Antibody Picoband®
View all CD40/TNFRSF5 Antibodies
SKU/Catalog Number
A00068-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD40/Cd40 Antibody Picoband® catalog # A00068-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD40/Cd40 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00068-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat CD40 recombinant protein (Position: V24-R193).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00068-1 is reactive to Cd40 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
31801 MW
Background of CD40/TNFRSF5
This CD40 gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00068-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat PC-12 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD40 using anti-CD40 antibody (A00068-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat PC-12 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40 antigen affinity purified polyclonal antibody (Catalog # A00068-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40 at approximately 36KD. The expected band size for CD40 is at 30KD.
Protein Target Info & Infographic
Gene/Protein Information For Cd40 (Source: Uniprot.org, NCBI)
Gene Name
Cd40
Full Name
Tumor necrosis factor receptor superfamily member 5
Weight
31801 MW
Alternative Names
CD40 molecule ;CD40 molecule, TNF receptor superfamily member 5 ;Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a ;Cd40 ;Tnfrsf5 ;rCG_32260 ; CD40 Bp50, CDW40, TNFRSF5, p50 CD40 molecule tumor necrosis factor receptor superfamily member 5|B cell surface CD40|B cell-associated molecule|CD40 molecule, TNF receptor superfamily member 5|CD40L receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd40, check out the Cd40 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD40/Cd40 Antibody Picoband® (A00068-1)
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Customer Q&As
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15 Customer Q&As for Anti-CD40/Cd40 Antibody Picoband®
Question
I see that the anti-CD40/Cd40 antibody A00068-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-05-06
Question
We ordered your anti-CD40/Cd40 antibody for WB on ovary a few years ago. I am using mouse, and We want to use the antibody for ELISA next. I would like examining ovary as well as leukocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
I looked at the website and datasheets of our anti-CD40/Cd40 antibody and it appears that A00068-1 has been validated on mouse in both WB and ELISA. Thus A00068-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-28
Question
My question regards to test anti-CD40/Cd40 antibody A00068-1 on rat left coronary artery for research purposes, then I may be interested in using anti-CD40/Cd40 antibody A00068-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
The products we sell, including anti-CD40/Cd40 antibody A00068-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-01
Question
Our team were satisfied with the WB result of your anti-CD40/Cd40 antibody. However we have been able to see positive staining in left coronary artery isoform i: cell membrane using this antibody. Is that expected? Could you tell me where is CD40 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
From literature, left coronary artery does express CD40. Generally CD40 expresses in isoform i: cell membrane. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161
Boster Scientific Support
Answered: 2019-11-08
Question
Does A00068-1 anti-CD40/Cd40 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
It shows on the product datasheet, A00068-1 anti-CD40/Cd40 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-16
Question
We have observed staining in rat left coronary artery. What should we do? Is anti-CD40/Cd40 antibody supposed to stain left coronary artery positively?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
According to literature left coronary artery does express CD40. According to Uniprot.org, CD40 is expressed in left coronary artery, kidney, ovary, leukocyte, lymphoma urinary bladder carcinoma, among other tissues. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161
Boster Scientific Support
Answered: 2019-07-18
Question
Please see the WB image, lot number and protocol we used for left coronary artery using anti-CD40/Cd40 antibody A00068-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-18
Question
Is there a BSA free version of anti-CD40/Cd40 antibody A00068-1 available?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD40/Cd40 antibody A00068-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-26
Question
Can you help my question with product A00068-1, anti-CD40/Cd40 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Banerjee
Verified customer
Asked: 2019-02-28
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00068-1 anti-CD40/Cd40 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-02-28
Question
Is this A00068-1 anti-CD40/Cd40 antibody reactive to the isotypes of CD40?
M. Wu
Verified customer
Asked: 2018-09-27
Answer
The immunogen of A00068-1 anti-CD40/Cd40 antibody is E. coli-derived rat CD40 recombinant protein (Position: V24-R193). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-27
Question
I was wanting to use your anti-CD40/Cd40 antibody for WB for rat left coronary artery on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat left coronary artery identification?
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
You can see on the product datasheet, A00068-1 anti-CD40/Cd40 antibody has been tested for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat left coronary artery in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-26
Question
Will anti-CD40/Cd40 antibody A00068-1 work for WB with left coronary artery?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
According to the expression profile of left coronary artery, CD40 is highly expressed in left coronary artery. So, it is likely that anti-CD40/Cd40 antibody A00068-1 will work for WB with left coronary artery.
Boster Scientific Support
Answered: 2018-09-04
Question
We are currently using anti-CD40/Cd40 antibody A00068-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
The anti-CD40/Cd40 antibody (A00068-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left coronary artery using anti-CD40/Cd40 antibody A00068-1. Let me know if you need anything else.
A. Wu
Verified customer
Asked: 2018-03-02
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-02
Question
Is a blocking peptide available for product anti-CD40/Cd40 antibody (A00068-1)?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
We do provide the blocking peptide for product anti-CD40/Cd40 antibody (A00068-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-15
