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Product Info Summary
| SKU: | M00885-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD34 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00885-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD34 Rabbit Monoclonal Antibody catalog # M00885-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD34 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00885-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BBH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD34
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00885-1 is reactive to CD34 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
40716 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00885-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: expression NIH/3T3 cell
IHC: human kidney tissue
ICC/IF: HUVEC cell
Validation Images & Assay Conditions
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Western blot analysis of CD34 in expression NIH/3T3 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human kidney, using CD34 Antibody.
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Immunofluorescent analysis of HUVEC cells, using CD34 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For CD34 (Source: Uniprot.org, NCBI)
Gene Name
CD34
Full Name
Hematopoietic progenitor cell antigen CD34
Weight
40716 MW
Superfamily
CD34 family
Alternative Names
Hematopoietic progenitor cell antigen CD34;CD34;CD34; CD34 CD34 molecule hematopoietic progenitor cell CD34|CD34
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD34, check out the CD34 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD34: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD34 Rabbit Monoclonal Antibody (M00885-1)
Hello CJ!
M00885-1 has been cited in 45 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A low molecular weight polysaccharide isolated from Agaricus blazei suppresses tumor growth and angiogenesis in vivo
Shuyuan Guo,Yusen Zhang,Yanmin Zhang,Fanhua Meng,Minghua Li,Zhendong Yu,Yun Chen,Guanghui Cui, "Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute
Species: Human,Rat
M00885-1 usage in article: APP:FLOW CYTOMETRY, SAMPLE:PBMC-5 CELL, DILUTION:NA
Peng L,Wang Y,Fei S,Wei C,Tong F,Wu G,Ma H,Dong X.The effect of combining Endostar with radiotherapy on blood vessels, tumor-associated macrophages, and T cells in brain metastases of Lewis lung cancer.Transl Lung Cancer Res.2020 Jun;9(3):745-760.doi:10.2
Species: Mouse
M00885-1 usage in article: APP:IF, SAMPLE:LUNG TUMOR TISSUE, DILUTION:NA
Liang C, Shangguan J, Yang L, Guo S. Downregulation of astrocyte elevated gene-1 expression inhibits the development of vasculogenic mimicry in gliomas. Exp Ther Med. 2021 Jan;21(1):22. doi:10.3892/etm. 2020. 9454. Epub 2020 Nov 6. PMID: 33235631; PMCID:
Species: Human, Rat
M00885-1 usage in article: APP:IHC, SAMPLE:BRAIN AND TUMOR TISSUE, DILUTION:1:200
Assefa F,Lim J,Kim JA,Ihn HJ,Lim S,Nam SH,Bae YC,Park EK. Secretoneurin, a Neuropeptide, Enhances Bone Regeneration in a Mouse Calvarial Bone Defect Model. Tissue Eng Regen Med.2020 Nov 3.doi: 10.1007/s13770-020-00304-1.Epub ahead of print.PMID:33145742.
Species: Mouse
M00885-1 usage in article: APP:IHC, SAMPLE:CALVARIAL BONE, DILUTION:1:500
SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary Giovanna Di Emidio, et al. Biochim Biophys Acta Mol Basis Dis. 2019 Jun 1;1865(6):1389-1401. doi: 10.1016/j.bbadis.2019.02.011. Epub 2019 Feb 13.
Dl-3-n-Butylphthalide treatment enhances hemodynamics and ameliorates memory deficits in rats with chronic cerebral hypoperfusion
Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase
Assessment of Vascularity in Hepatic Alveolar Echinococcosis: Comparison of Quantified Dual-Energy CT with Histopathologic Parameters
Arginine ADP-ribosyltransferase 1 promotes angiogenesis in colorectal cancer via the PI3K/Akt pathway
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-CD34 Rabbit Monoclonal Antibody
Question
We have seen staining in human megakaryoblast. What should we do? Is anti-CD34 Rabbit Monoclonal antibody supposed to stain megakaryoblast positively?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
Based on literature megakaryoblast does express CD34. Based on Uniprot.org, CD34 is expressed in esophagus, bone marrow, megakaryoblast, heart, placenta, skin, among other tissues. Regarding which tissues have CD34 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1370171
Placenta, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-16
Question
My boss were happy with the WB result of your anti-CD34 Rabbit Monoclonal antibody. However we have seen positive staining in placenta membrane using this antibody. Is that expected? Could you tell me where is CD34 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
Based on literature, placenta does express CD34. Generally CD34 expresses in membrane. Regarding which tissues have CD34 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1370171
Placenta, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-27
Question
Our lab used your anti-CD34 Rabbit Monoclonal antibody for IHC on placenta in a previous experiment. I am using mouse, and I plan to use the antibody for WB next. I am interested in examining placenta as well as heart in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
I viewed the website and datasheets of our anti-CD34 Rabbit Monoclonal antibody and it appears that M00885-1 has been tested on mouse in both IHC and WB. Thus M00885-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-06-05
Question
We are currently using anti-CD34 Rabbit Monoclonal antibody M00885-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-27
Answer
The anti-CD34 Rabbit Monoclonal antibody (M00885-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-27
