Product Info Summary
SKU: | PB9053 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD34 Antibody Picoband™
SKU/Catalog Number
PB9053
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD34 Antibody Picoband™ catalog # PB9053. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD34 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9053)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD34 recombinant protein (Position: T151-L385). Human CD34 shares 79% amino acid (aa) sequence identity with mouse CD34.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9053 is reactive to CD34 in Human, Rat
Applications
PB9053 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
105 kDa
Calculated molecular weight
40716 MW
Background of CD34
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
![pb9053 cd34 primary antibodies wb testing 1 pb9053 cd34 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/p/b/pb9053-cd34-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of CD34 using anti-CD34 antibody (PB9053).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (Catalog # PB9053) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34 at approximately 105KD. The expected band size for CD34 is at 105KD.
![pb9053 cd34 primary antibodies if testing 10 pb9053 cd34 primary antibodies if testing 10](https://www.bosterbio.com/media/catalog/product/p/b/pb9053-cd34-primary-antibodies-if-testing-10.jpg)
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Figure 10. IF analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![pb9053 cd34 primary antibodies ihc testing 2 pb9053 cd34 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/p/b/pb9053-cd34-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![pb9053 cd34 primary antibodies ihc testing 3 pb9053 cd34 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/p/b/pb9053-cd34-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![pb9053 cd34 primary antibodies ihc testing 4 pb9053 cd34 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/p/b/pb9053-cd34-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![pb9053 cd34 primary antibodies ihc testing 5 pb9053 cd34 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/p/b/pb9053-cd34-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![pb9053 cd34 primary antibodies ihc testing 6 pb9053 cd34 primary antibodies ihc testing 6](https://www.bosterbio.com/media/catalog/product/p/b/pb9053-cd34-primary-antibodies-ihc-testing-6.jpg)
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Figure 6. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![pb9053 cd34 primary antibodies ihc testing 7 pb9053 cd34 primary antibodies ihc testing 7](https://www.bosterbio.com/media/catalog/product/p/b/pb9053-cd34-primary-antibodies-ihc-testing-7.jpg)
Click image to see more details
Figure 7. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![pb9053 cd34 primary antibodies ihc testing 8 pb9053 cd34 primary antibodies ihc testing 8](https://www.bosterbio.com/media/catalog/product/p/b/pb9053-cd34-primary-antibodies-ihc-testing-8.jpg)
Click image to see more details
Figure 8. IHC analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![pb9053 cd34 primary antibodies if testing 9 pb9053 cd34 primary antibodies if testing 9](https://www.bosterbio.com/media/catalog/product/p/b/pb9053-cd34-primary-antibodies-if-testing-9.jpg)
Click image to see more details
Figure 9. IF analysis of CD34 using anti-CD34 antibody (PB9053).
CD34 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CD34 (Source: Uniprot.org, NCBI)
Gene Name
CD34
Full Name
Hematopoietic progenitor cell antigen CD34
Weight
40716 MW
Superfamily
CD34 family
Alternative Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1 CD34 CD34 molecule hematopoietic progenitor cell antigen CD34|CD34 antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD34, check out the CD34 Infographic
![CD34 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD34: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD34 Antibody Picoband™ (PB9053)
Hello CJ!
PB9053 has been cited in 88 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Effects of Inactive Platelet-rich Plasma at Different Injection Time on Prefabricated Flap Viability in Rabbits
An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties
Dahai Dong,Yu Yao,Jinlei Song,Lijiang Sun,Guiming Zhang,"Cancer-Associated Fibroblasts Regulate Bladder Cancer Invasion and Metabolic Phenotypes through Autophagy",Disease Markers,vol.2021,Article ID 6645220,8 pages,2021.https://doi.org/10.1155/2021/6645220
Species: Human
PB9053 usage in article: APP:IF, SAMPLE:HFF CELL, DILUTION:NA
Zhu WQ,Cai NN,Jiang Y,Yang R,Shi JZ,Zhu CL,Zhang BY,Tang B,Zhang XM.Survivable potential of germ cells after trehalose cryopreservation of bovine testicular tissues.Cryobiology.2021 May 11:S0011-2240(21)00081-X.doi:10.1016/j.cryobiol.2021.05.001.Epub ahead of print.PMID:33989617.
Species: Bull
PB9053 usage in article: APP:IF, SAMPLE:TESTICULAR TISSUE, DILUTION:1:200
Xuebing B,Ruizhi W,Yue Z,Chunhua L,Yonghong S,Yingxin Z,Baitao D,Tarique I,Ping Y,Qiusheng C. Tissue Micro-channels Formed by Collagen Fibers and their Internal Components: Cellular Evidence of Proposed Meridian Conduits in Vertebrate Skin.Microsc Microanal.2020 Oct;26(5):1069-1075.doi:10.101 7/S1431927620024381.PMID:32883394.
Species: Frog,Turtle,Chicken,Sheep
PB9053 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:1:150
Ma C,Liu G,Liu W,Xu W,Li H,Piao S,Sui Y,Feng W.CXCL1 stimulates decidual angiogenesis via the VEGF-A pathway during the first trimester of pregnancy.Mol Cell Biochem.2021 Mar 26.doi:10.1007/s11010-021-04137-x.Epub ahead of print.PMID:33770315.
Species: Human,Mouse
PB9053 usage in article: APP:ICC, SAMPLE:DECIDUA TISSE, DILUTION:1:500
Shuyuan Guo,Yusen Zhang,Yanmin Zhang,Fanhua Meng,Minghua Li,Zhendong Yu,Yun Chen,Guanghui Cui, "Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute
Species: Human,Rat
Assefa F,Lim J,Kim JA,Ihn HJ,Lim S,Nam SH,Bae YC,Park EK. Secretoneurin, a Neuropeptide, Enhances Bone Regeneration in a Mouse Calvarial Bone Defect Model. Tissue Eng Regen Med.2020 Nov 3.doi: 10.1007/s13770-020-00304-1.Epub ahead of print.PMID:33145742.
Species: Mouse
PB9053 usage in article: APP:IHC, SAMPLE:CALVARIAL BONE, DILUTION:1:500
Dipalmitoylphosphatidic acid inhibits tumor growth in triple-negative breast cancer
Cardiac-derived CTRP9 protects against myocardial ischemia/reperfusion injury via calreticulin-dependent inhibition of apoptosis
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