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Product Info Summary
| SKU: | EK0700 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CD36/SR-B3/GP4 ELISA Kit PicoKine®
View all CD36/SR-B3 ELISA kits
SKU/Catalog Number
EK0700
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD36/SR-B3 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD36 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD36/SR-B3/GP4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0700)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: G30-N439
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0700 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0700 is reactive to CD36 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0700 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD36/SR-B3
CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0700-CAP | Anti-Human CD36 Pre-coated 96-well strip microplate | 1 |
| EK0700-ST | Human CD36 Standard | 2 vials, 10 ng/tube |
| EK0700-DA | Human CD36 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.036 | 0.110 | 0.183 | 0.230 | 0.386 | 0.596 | 0.991 | 1.861 |
Data Example Images
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Human CD36/SR-B3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD36/SR-B3, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 156 pg/ml..
Some articles we found to cite concentrations of CD36/SR-B3 in samples: 25619588, 24766787 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD36/SR-B3/GP4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 329 | 1274 | 5223 | 298 | 1195 | 5251 |
| Standard deviation | 23.03 | 76.44 | 360.38 | 21.45 | 81.26 | 456.83 |
| CV (%) | 7% | 6% | 6.9% | 7.2% | 6.8% | 8.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD36/SR-B3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 329 | 358 | 360 | 320 | 341 | 17.55 | 5.1% |
| Sample 2 | 1274 | 1304 | 1194 | 1283 | 1263 | 41.71 | 3.3% |
| Sample 3 | 5223 | 5923 | 6080 | 5774 | 5750 | 322.92 | 5.6% |
Protein Target Info & Infographic
Gene/Protein Information For CD36 (Source: Uniprot.Org, NCBI)
Gene Name
CD36
Full Name
Platelet glycoprotein 4
Weight
53053 MW
Superfamily
CD36 family
Alternative Names
Platelet glycoprotein 4;Fatty acid translocase;FAT;Glycoprotein IIIb;GPIIIB;Leukocyte differentiation antigen CD36;PAS IV;PAS-4;Platelet collagen receptor;Platelet glycoprotein IV;GPIV;Thrombospondin receptor;CD36;CD36;GP3B, GP4; CD36 BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, SCARB3 CD36 molecule platelet glycoprotein 4|CD36 (collagen type I receptor, thrombospondin receptor)|CD36 molecule (thrombospondin receptor)|GPIIIB|PAS IV|PAS-4 protein|cluster determinant 36|fatty acid translocase|glycoprotein IIIb|leukocyte differentiation CD36|platelet glycoprotein IV|scavenger receptor class B, member 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD36, check out the CD36 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD36: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD36/SR-B3/GP4 ELISA Kit PicoKine® (EK0700)
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No publications found for EK0700
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human CD36/SR-B3/GP4 ELISA Kit PicoKine®
Question
Q: how do I analyze ELISA data? I have obtained CD36 level in plasma.
L. Varadkar
Verified customer
Asked: 2020-07-07
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-07-07
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CD36 using the EK0700 Human CD36 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-03-14
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-03-14
Question
Q: Can CD36 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
K. Miller
Verified customer
Asked: 2020-03-02
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-03-02
Question
Can EK0700 detect soluble CD36?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
EK0700 can detect soluble CD36.
Boster Scientific Support
Answered: 2020-02-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-08-06
Question
Q: if the enzyme conjugated CD36 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD36 antigen?
C. King
Verified customer
Asked: 2019-05-26
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-05-26
Question
Q: how can I thaw whole blood sample for CD36 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD36 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-09-10
Question
Q: can you suggest the dilution ratio of serum samples for detection of CD36 in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD36 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-02-14
