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Product Info Summary
| SKU: | PB9055 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Mast Cell Chymase/CMA1 Antibody Picoband®
View all Chymase/CMA1/Mast Cell Chymase Antibodies
SKU/Catalog Number
PB9055
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mast Cell Chymase/CMA1 Antibody Picoband® catalog # PB9055. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mast Cell Chymase/CMA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9055)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CMA1 recombinant protein (Position: I22-N247). Human CMA1 shares 75% and 74% amino acid (aa) sequences identity with mouse and rat CMA1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9055 is reactive to CMA1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
27325 MW
Background of Chymase/CMA1/Mast Cell Chymase
Chymase is a major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. Chymase is mapped to 14q12. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9055 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Positive Control
WB: human PC-3 whole cell, human HepG2 whole cell, rat liver tissue, mouse HEPA1-6 whole cell,
IHC: human placenta tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of CMA1 using anti-CMA1 antibody (PB9055).
CMA1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CMA1 Antibody (PB9055) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. Western blot analysis of CMA1 using anti-CMA1 antibody (PB9055).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse HEPA1-6 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CMA1 antigen affinity purified polyclonal antibody (Catalog # PB9055) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CMA1 at approximately 35KD. The expected band size for CMA1 is at 27KD.
Protein Target Info & Infographic
Gene/Protein Information For CMA1 (Source: Uniprot.org, NCBI)
Gene Name
CMA1
Full Name
Chymase
Weight
27325 MW
Superfamily
peptidase S1 family
Alternative Names
Chymase;3.4.21.39;Alpha-chymase;Mast cell protease I;CMA1;CYH, CYM; CMA1 CYH, MCT1, chymase chymase 1 chymase|alpha-chymase|chymase 1 preproprotein transcript E|chymase 1 preproprotein transcript I|chymase 1, mast cell|chymase, heart|chymase, mast cell|mast cell protease I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CMA1, check out the CMA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CMA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mast Cell Chymase/CMA1 Antibody Picoband® (PB9055)
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2 Customer Q&As for Anti-Mast Cell Chymase/CMA1 Antibody Picoband®
Question
We are currently using anti-Mast Cell Chymase/CMA1 antibody PB9055 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
The anti-Mast Cell Chymase/CMA1 antibody (PB9055) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-30
Question
Can PB9055 detect active, inactive (or both) forms of CMA1?
Verified customer
Asked: 2019-06-21
Answer
Unfortunately, we are uncertain if the Anti-Mast Cell Chymase/CMA1 Antibody Picoband™ (PB9055) detect active, inactive (or both) forms of CMA1. The immunogen sequence is I22-N247 which contains active sites. Our lab hasn't perform relevant tests to validate the detection form of the antibody.
Boster Scientific Support
Answered: 2019-06-21
