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Product Info Summary
| SKU: | FC00137-PerCP-Cy5.5 |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Cynomolgus monkey, Human, Baboon, Rhesus monkey |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD14 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated
SKU/Catalog Number
FC00137-PerCP-Cy5.5
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD14 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Catalog# FC00137-PerCP-Cy5.5). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD14 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00137-PerCP-Cy5.5)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: 26ic
Isotype
IgG2b,k
Immunogen
Cultured human peripheral blood monocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
FC00137-PerCP-Cy5.5 is reactive to CD14 in Cynomolgus monkey, Human, Baboon, Rhesus monkey
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
40076 MW
Background of CD14
CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00137-PerCP-Cy5.5 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Monocytes gated PBMCs stained with PerCP-Cyanine5.5 conjugated antihuman CD14 (clone 26iC) (red histogram). Monocytes gated PBMCs stained with PerCP-Cyanine5.5 conjugated mouse IgG2b, k isotype control (blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For CD14 (Source: Uniprot.org, NCBI)
Gene Name
CD14
Full Name
Monocyte differentiation antigen CD14
Weight
40076 MW
Alternative Names
Monocyte differentiation antigen CD14 ;Myeloid cell-specific leucine-rich glycoprotein;CD14;Monocyte differentiation antigen CD14, urinary form;Monocyte differentiation antigen CD14, membrane-bound form;CD14; CD14 CD14 molecule monocyte differentiation CD14|myeloid cell-specific leucine-rich glycoprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD14, check out the CD14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD14 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC00137-PerCP-Cy5.5)
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No publications found for FC00137-PerCP-Cy5.5
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Anti-human CD14 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated
Question
My question regarding product FC00137-PerCP-Cy5.5, anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00137-PerCP-Cy5.5 anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-08
Question
I was wanting to use your anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated for Flow Cytometry for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
It shows on the product datasheet, FC00137-PerCP-Cy5.5 anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-14
Question
I see that the anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00137-PerCP-Cy5.5 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-12
Question
Would anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00137-PerCP-Cy5.5 work for Flow Cytometry with liver?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
According to the expression profile of liver, CD14 is highly expressed in liver. So, it is likely that anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00137-PerCP-Cy5.5 will work for Flow Cytometry with liver.
Boster Scientific Support
Answered: 2019-06-12
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00137-PerCP-Cy5.5. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-19
Question
Please see the WB image, lot number and protocol we used for liver using anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00137-PerCP-Cy5.5. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-20
Question
Will FC00137-PerCP-Cy5.5 anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-04-11
Answer
As indicated on the product datasheet, FC00137-PerCP-Cy5.5 anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-04-11
Question
My question regards to test anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00137-PerCP-Cy5.5 on human liver for research purposes, then I may be interested in using anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00137-PerCP-Cy5.5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
The products we sell, including anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00137-PerCP-Cy5.5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-24
Question
Do you have a BSA free version of anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00137-PerCP-Cy5.5 available?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00137-PerCP-Cy5.5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-01
Question
Is a blocking peptide available for product anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC00137-PerCP-Cy5.5)?
G. Moore
Verified customer
Asked: 2015-01-09
Answer
We do provide the blocking peptide for product anti-human CD14 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC00137-PerCP-Cy5.5). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-01-09
