Key=store_id
Key=entity_id, value=55081
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Key=sku, value=M00223-1
Key=has_options, value=1
Key=required_options, value=1
Key=created_at, value=2019-02-27 15:13:51
Key=updated_at, value=2024-09-26 01:02:12
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Anti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4)
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Key=template, value=antibodies
Key=host, value=Mouse
Key=googleshopping_exclude, value=No
Key=yoast_keyword_score, value=-1325
Key=yoast_content_score, value=30
Key=badge, value=free-antibody-validation
Key=price, value=370.0000
Key=weight, value=1.0000
Key=meta_title, value=Anti-BRG1 Antibody Picoband® (M00223-1) (monoclonal, 3F4)
Key=meta_description, value=Boster Bio Anti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4) catalog # M00223-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Key=image, value=/m/0/m00223-1-brg1-primary-antibodies-wb-testing-1.jpg
Key=small_image, value=/m/0/m00223-1-brg1-primary-antibodies-wb-testing-1.jpg
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Key=url_key, value=anti-brg1-picoband-trade-antibody-monoclonal-m00223-1-boster
Key=url_path, value=anti-brg1-picoband-trade-antibody-monoclonal-m00223-1-boster
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Key=image_label, value=Figure 1. Western blot analysis of BRG1 using anti-BRG1 antibody (M00223-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of samp
Key=small_image_label, value=Figure 1. Western blot analysis of BRG1 using anti-BRG1 antibody (M00223-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of samp
Key=thumbnail_label, value=Figure 1. Western blot analysis of BRG1 using anti-BRG1 antibody (M00223-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of samp
Key=msrp_display_actual_price_type, value=
Key=gift_message_available, value=No
Key=size, value=100 μg/vial
Key=protein_name, value=Glial fibrillary acidic protein
Key=gene_full_name, value=SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Key=uniprot_id, value=P51532
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Key=sensitivity
Key=applications, value=Flow Cytometry, IHC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=0
Key=cross_reactivity, value=No cross-reactivity with other proteins.
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
Key=clone_number, value=3F4
Key=predicted_reactivity
Key=source_company, value=Boster M00223-1
Key=isotype, value=Mouse IgG1
Key=product_category, value=Primary Antibodies, Monoclonal Antibodies
Key=research_category, value=Calcium Channels, Calcium Signaling, Cancer, Cancer Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Neuroscience, Neurotransmission, Pathways and Processes, Signal Transduction, Signaling Pathway
Key=clonality, value=Monoclonal
Key=swatch_image, value=/antibody/ek0660-2-ELISA-mouse-axl-picokine-elisa-kit.jpg
Key=rating_value, value=100
Key=review_count, value=11
Key=phospho_site
Key=mp_exclude_sitemap, value=Yes
Key=yoast_facebook_image, value=/m/0/m00195-1-itch-primary-antibodies-wb-testing-1.jpg
Key=yoast_twitter_image, value=/m/0/m00195-1-itch-primary-antibodies-wb-testing-1.jpg
Key=cj_related_products, value=Atp-dependent Chromatin Remodeling:A00223-1^Chromatin Organization:A01312-Dyl550^Growth Arrest:A00173T187-1^Rhabdoid Tumor:A00223-1|PROTQ9HBD4
Key=description, value=Boster Bio Anti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4) catalog # M00223-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4) catalog # M00223-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4)
Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=Transcription activator BRG1 also known as ATP-dependent helicase SMARCA4 is a protein that in humans is encoded by the SMARCA4 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Key=gene_name, value=SMARCA4
Key=synonyms, value=Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK1; CDC2P; CDC28A; CDKN1; P34CDC2
Key=application_details, value=Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Key=subcellular_localization, value=Nucleus.
Key=tissue_specificity, value=Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).
Key=application_notes, value=Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=immunogen, value=E. coli-derived human BRG1 recombinant protein (Position: Q555-E763).
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=181 kDa
Key=custom_attribute_2, value=WB: human Raji whole cell,, human K562 whole cell,, human HL-60 whole cell,, human Caco-2 whole cell,, human Hela whole cell,, human HepG2 whole cell,, rat brain tissue,, mouse brain tissue,, mouse lung tissue
IHC: human lung cancer tissue, human rectal cancer tissue, human rectal cancer tissue, rat intestine tissue
FCM: U20S cell
Key=protein_function, value=Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development, a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Binds via DLX1 to enhancers located in the intergenic region between DLX5 and DLX6 and this binding is stabilized by the long non-coding RNA (lncRNA) Evf2. Binds to RNA in a promiscuous manner. Binding to RNAs including lncRNA Evf2 leads to inhibition of SMARCA4 ATPase and chromatin remodeling activities.
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