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Product Info Summary
| SKU: | A00173T187-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-p27 (T187) CDKN1B Antibody
SKU/Catalog Number
A00173T187-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-p27 (T187) CDKN1B Antibody catalog # A00173T187-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-p27 (T187) CDKN1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00173T187-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human p27 Kip1 around the phosphorylation site of Thr187. AA range:149-198
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00173T187-1 is reactive to CDKN1B in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
22073 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00173T187-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-p27 (T187) polyclonal antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p27 Kip1 (Phospho-Thr187) antibody
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Western blot analysis of lysates from HeLa cells treated with EGF or IFN-α, using p27 Kip1 (Phospho-Thr187) antibody. The lane on the left is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 antibody was diluted at 1:200 (4° overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Protein Target Info & Infographic
Gene/Protein Information For CDKN1B (Source: Uniprot.org, NCBI)
Gene Name
CDKN1B
Full Name
Cyclin-dependent kinase inhibitor 1B
Weight
22073 MW
Superfamily
CDI family
Alternative Names
Cyclin-dependent kinase inhibitor 1B;Cyclin-dependent kinase inhibitor p27 CDKN1B CDKN4, KIP1, MEN1B, MEN4, P27KIP1 cyclin dependent kinase inhibitor 1B cyclin-dependent kinase inhibitor 1B|cyclin-dependent kinase inhibitor 1B (p27, Kip1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKN1B, check out the CDKN1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKN1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-p27 (T187) CDKN1B Antibody (A00173T187-1)
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