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Product Info Summary
| SKU: | M00223 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BRG1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00223
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-BRG1 Rabbit Monoclonal Antibody catalog # M00223. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BRG1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00223)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GFB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human BRG1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00223 is reactive to SMARCA4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
58 kDa
Calculated molecular weight
184646 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00223 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
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Western blot analysis of BRG1 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For SMARCA4 (Source: Uniprot.org, NCBI)
Gene Name
SMARCA4
Full Name
Transcription activator BRG1
Weight
184646 MW
Superfamily
SNF2/RAD54 helicase family
Alternative Names
Transcription activator BRG1;3.6.4.-;ATP-dependent helicase SMARCA4;BRG1-associated factor 190A;BAF190A;Mitotic growth and transcription activator;Protein BRG-1;Protein brahma homolog 1;SNF2-beta;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4;SMARCA4;BAF190A, BRG1, SNF2B, SNF2L4; SMARCA4 BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 transcription activator BRG1|ATP-dependent helicase SMARCA4|BRG1-associated factor 190A|BRM/SWI2-related gene 1|SNF2-like 4|brahma protein-like 1|global transcription activator homologous sequence|homeotic gene regulator|mitotic growth and transcription activator|nuclear protein GRB1|protein BRG-1|protein brahma homolog 1|sucrose nonfermenting-like 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMARCA4, check out the SMARCA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMARCA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRG1 Rabbit Monoclonal Antibody (M00223)
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No publications found for M00223
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Customer Q&As
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17 Customer Q&As for Anti-BRG1 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-BRG1 Rabbit Monoclonal antibody for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
You can see on the product datasheet, M00223 anti-BRG1 Rabbit Monoclonal antibody has been tested for ChIP, IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-30
Question
My boss were happy with the WB result of your anti-BRG1 Rabbit Monoclonal antibody. However we have seen positive staining in lung nucleus using this antibody. Is that expected? Could you tell me where is SMARCA4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
From what I have seen in literature, lung does express SMARCA4. Generally SMARCA4 expresses in nucleus. Regarding which tissues have SMARCA4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal brain, Pubmed ID: 8208605
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 18386774
Boster Scientific Support
Answered: 2020-03-20
Question
My question regarding product M00223, anti-BRG1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00223 anti-BRG1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-03
Question
I see that the anti-BRG1 Rabbit Monoclonal antibody M00223 works with WB, what is the protocol used to produce the result images on the product page?
D. Kulkarni
Verified customer
Asked: 2019-08-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-14
Question
Does M00223 anti-BRG1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
You can see on the product datasheet, M00223 anti-BRG1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-29
Question
We need using your anti-BRG1 Rabbit Monoclonal antibody for interleukin-7 signaling studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
Thanks for your inquiry. This M00223 anti-BRG1 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for ChIP, IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-27
Question
Is this M00223 anti-BRG1 Rabbit Monoclonal antibody reactive to the isotypes of SMARCA4?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
The immunogen of M00223 anti-BRG1 Rabbit Monoclonal antibody is A synthesized peptide derived from human BRG1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-18
Question
We are currently using anti-BRG1 Rabbit Monoclonal antibody M00223 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
The anti-BRG1 Rabbit Monoclonal antibody (M00223) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-21
Question
We have been able to see staining in human cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-BRG1 Rabbit Monoclonal antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2019-03-01
Answer
From literature cervix carcinoma erythroleukemia does express SMARCA4. From Uniprot.org, SMARCA4 is expressed in tendon of biceps brachii, fetal brain, lung, cervix carcinoma, embryonic kidney, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SMARCA4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal brain, Pubmed ID: 8208605
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 18386774
Boster Scientific Support
Answered: 2019-03-01
Question
Our lab used your anti-BRG1 Rabbit Monoclonal antibody for WB on leukemic t-cell a few months ago. I am using mouse, and We intend to use the antibody for IP next. I am interested in examining leukemic t-cell as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2018-04-10
Answer
I looked at the website and datasheets of our anti-BRG1 Rabbit Monoclonal antibody and I see that M00223 has been validated on mouse in both WB and IP. Thus M00223 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-10
Question
Do you have a BSA free version of anti-BRG1 Rabbit Monoclonal antibody M00223 available?
Verified Customer
Verified customer
Asked: 2018-01-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-BRG1 Rabbit Monoclonal antibody M00223 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-17
Question
I am interested in to test anti-BRG1 Rabbit Monoclonal antibody M00223 on human leukemic t-cell for research purposes, then I may be interested in using anti-BRG1 Rabbit Monoclonal antibody M00223 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Li
Verified customer
Asked: 2017-07-20
Answer
The products we sell, including anti-BRG1 Rabbit Monoclonal antibody M00223, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-20
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-BRG1 Rabbit Monoclonal antibody M00223. Please let me know if you require anything else.
N. Evans
Verified customer
Asked: 2017-03-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-13
Question
Does anti-BRG1 Rabbit Monoclonal antibody M00223 work for WB with leukemic t-cell?
A. Jackson
Verified customer
Asked: 2015-08-03
Answer
According to the expression profile of leukemic t-cell, SMARCA4 is highly expressed in leukemic t-cell. So, it is likely that anti-BRG1 Rabbit Monoclonal antibody M00223 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2015-08-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-BRG1 Rabbit Monoclonal antibody M00223. Let me know if you need anything else.
M. Zhao
Verified customer
Asked: 2014-01-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-01-31
Question
Is a blocking peptide available for product anti-BRG1 Rabbit Monoclonal antibody (M00223)?
M. Collins
Verified customer
Asked: 2013-12-12
Answer
We do provide the blocking peptide for product anti-BRG1 Rabbit Monoclonal antibody (M00223). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-12-12
Question
Would anti-BRG1 Rabbit Monoclonal antibody M00223 work on horse IP with leukemic t-cell?
V. Krishna
Verified customer
Asked: 2013-10-31
Answer
Our lab technicians have not validated anti-BRG1 Rabbit Monoclonal antibody M00223 on horse. You can run a BLAST between horse and the immunogen sequence of anti-BRG1 Rabbit Monoclonal antibody M00223 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse leukemic t-cell in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-10-31
